Diagenode

24 UDI for tagmented libraries - Set IV

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Catalog Number
Format
Price
C01011038
24 UDI
$170.00

The 24 UDI for tagmented libraries includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to 24 samples for sequencing on Illumina platforms. These UDI are designed and validated to be used with μChIPmentation for Histones (Cat. No. C01011011), ChIPmentation Kit for Histones (Cat. No. C01011009), TAG Kit for ChIPmentation (Cat. No. C01011030), ATAC-seq Kit (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other tagmentation-based library preparation protocols, such as CUT&Tag technologies.

4 sets of UDI for tagmented libraries are available:

24 UDI for tagemented libraries - Set I
24 UDI for tagmented libraries - Set II
24 UDI for tagmented libraries -  Set III
24 UDI for tagmented libraries - Set IV

Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 96.

Features:

  • Multiplexing: up to 96 samples (using all 4 sets simultaneously)
  • Allow for identification of index hopping
  • Compatibility: tagmentation-based library preparation protocols

  • Characteristics

    The 24 UDI (Unique dual indexes) for tagmented libraries sets are compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.

    The 24 UDI for tagmented libraries provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.

    Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used.

  •  Documents
    Primer indexes for tagmented libraries MANUAL
    The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adapt...
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  •  Publications

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