Diagenode

DNA Methylation

Methylbinding domain protein

The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs.

Diagenode’s MethylCap® kit enables high enrichment of double-stranded DNA and a differential fractionation in function of the methylated CpG density. Fractionation reduces the complexity of samples and makes subsequent next generation sequencing easier. Prior to the MethylCap assay, DNA is first extracted and sheared using the Bioruptor® Sonicator.

Overview

Recommended products for Methylbinding domain protein

  • Bioruptor Pico
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    Bioruptor® Pico sonication device
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    MethylCap kit
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    C02030032
    Premium RRBS kit (24 rxns) - replaced by Premiu...
  • Methylation kit icon
    C02020011
    Auto MethylCap kit
  • Bioruptor pico next gen sequencing
    B01060010
    Bioruptor® Pico sonication device

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