Diagenode

DNA Methylation

Complete solutions for DNA methylation studies

Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.

DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.



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    5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.


Main DNA methylation technologies

Overview of the three main approaches for studying DNA methylation.

  1. Chemical modification with bisulfite – Bisulfite conversion
  2. Enrichment of methylated DNA (including MeDIP and MBD)
  3. Treatment with methylation-sensitive or dependent restriction enzymes

 

Description Features
Bisulfite conversion Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.
  • Single nucleotide resolution
  • Quantitative analysis - methylation rate (%)
  • Gold standard and well studied
  • Compatible with automation
Methylated DNA enrichment (Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.
  • Resolution depends on the fragment size of the enriched methylated DNA (300 bp)
  • Qualitative analysis
  • Compatible with automation
Restriction enzyme-based digestion Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.
  • Determination of methylation status is limited by the enzyme recognition site
  • Easy to use

Recommended products for DNA Methylation

Cat. No.ProductFormatPrice
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $510.00
C02010021 MagMeDIP kit
Perform MeDIP (-seq) (Methylated DNA Immunoprecipitation sequencing) to estimate DNA methylation status of your sample using the 5-methylcytosine antibody. F...
48 rxns $615.00
C02020010 MethylCap kit
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methylbinding domain) protein . This procedure has first been optimi...
48 rxns $570.00
C02030030 Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
50 rxns $200.00
C02030032 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...
24 rxns $1,455.00
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using ...
8 rxns $745.00
C02040011 5-hmC, 5-mC & cytosine DNA standard pack for hMeDIP
The hydroxymethylated DNA standard is a linear dsDNA that has been produced by PCR using the Diagenode’s MethylTaq DNA polymerase and Diageno...
40 rxns $180.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $510.00
C02010033 Auto hMeDIP kit x16 (monoclonal rat antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $510.00
C02010035 Auto hMeDIP kit x16 (polyclonal rabbit antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $510.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $200.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $99.00
C02010030 hMeDIP kit x16 (monoclonal rat antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $510.00
C02010032 hMeDIP kit x16 (polyclonal rabbit antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $510.00
C02020012 MethylCap protein
The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs. It consists of the methyl binding domain (MBD) of human MeC...
50 rxns $465.00

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