Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.
DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.
Overview of the three main approaches for studying DNA methylation.
| Description | Features | |
|---|---|---|
| Bisulfite conversion | Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution. |
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| Methylated DNA enrichment | (Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA. |
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| Restriction enzyme-based digestion | Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites. |
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| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02010031 |
hMeDIP kit x16 (monoclonal mouse antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto... |
16 rxns | $535.00 | |
| B03000002 |
IP-Star® Compact Automated System The IP-Star® Compact benchtop automation system is the latest innovation in epigenetics sample preparation automation, replacing the numerous manual, err... |
1 unit | ||
| C02010021 |
MagMeDIP qPCR Kit Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo... |
48 rxns | $645.00 | |
| C02010040 |
MagMeDIP-seq Package *Use the MAGPACK02 promo code in your online order or by clicking on the banner. Sensitive tumour detection and classification using plasma cel... |
10 rxns | $1,365.00 | |
| C02020010 |
MethylCap kit The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methylbinding domain) protein . This procedure has first been optimi... |
48 rxns | $600.00 | |
| C02030030 |
Premium Bisulfite kit Make your Bisulfite conversion now in only 90 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r... |
50 rxns | $210.00 | |
| C02030032 |
Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit *Terms and conditions: Offer valid on RRBS Kit 24 rxns C02030032 ansd 96 rxns C02030033 with the promo code RRBS20. Offer valid until July 31st, 2020. R... |
24 rxns | $1,530.00 | |
| C02030034 |
Premium Whole Genome Bisulfite Sequencing (WGBS) kit Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes Compatible with ChIP-Bis-Seq - Com... |
8 rxns | $780.00 | |
| C02040011 |
5-hmC, 5-mC & cytosine DNA standard pack for hMeDIP The hydroxymethylated DNA standard is a linear dsDNA that has been produced by PCR using the Diagenode’s MethylTaq DNA polymerase and Diageno... |
40 rxns | $190.00 | |
| C02010034 |
Auto hMeDIP kit x16 (monoclonal mouse antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy... |
16 rxns | $535.00 | |
| C02010033 |
Auto hMeDIP kit x16 (monoclonal rat antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy... |
16 rxns | $535.00 | |
| C02010035 |
Auto hMeDIP kit x16 (polyclonal rabbit antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy... |
16 rxns | $535.00 | |
| C02030031 |
Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate... |
40 rxns | $210.00 | |
| C02030035 |
Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S... |
8 rxns | $105.00 | |
| C02010030 |
hMeDIP kit x16 (monoclonal rat antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto... |
16 rxns | $535.00 | |
| C02010032 |
hMeDIP kit x16 (polyclonal rabbit antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto... |
16 rxns | $535.00 | |
| C02020012 |
MethylCap protein The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs. It consists of the methyl binding domain (MBD) of human MeC... |
100 µg | $490.00 |
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