Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.
DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.
Overview of the three main approaches for studying DNA methylation.
| Description | Features | |
|---|---|---|
| Bisulfite conversion | Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution. |
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| Methylated DNA enrichment | (Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA. |
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| Restriction enzyme-based digestion | Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites. |
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| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02010031 |
hMeDIP kit x16 (monoclonal mouse antibody) The hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysi... |
16 rxns | $690.00 | |
| C02010021 |
MagMeDIP Kit Perform MeDIP (Methylated DNA Immunoprecipitation) followed by qPCR or NGS to estimate DNA methylation status of your sample using a highly sensitive 5-met... |
48 rxns (IP) | $770.00 | |
| C02020010 |
MethylCap kit The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA ... |
48 rxns | ||
| C02030030 |
Premium Bisulfite kit Make your Bisulfite conversion now in only 60 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion... |
50 rxns | $245.00 | |
| C02030036 |
Premium RRBS kit V2 RRBS for low DNA amounts and accurate analysis Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the... |
24 rxns | $1,590.00 | |
| C02030037 |
Premium RRBS kit V2 x96 RRBS for low DNA amounts and accurate analysis Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the s... |
96 rxns | $3,745.00 | |
| C02010034 |
Auto hMeDIP kit x16 (monoclonal mouse antibody) The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation anal... |
16 rxns | $690.00 | |
| C02030031 |
Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate... |
40 rxns | $245.00 | |
| C02030035 |
Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S... |
8 rxns | $125.00 | |
| C02040018 |
DNA hydroxymethylation control package The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer set that can be added to the DNA... |
48 rxns | $155.00 | |
| C02040012 |
DNA methylation control package The DNA methylation control package includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer ... |
40 rxns | $310.00 | |
| C02040019 |
DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets th... |
48 rxns | $285.00 | |
| G02180000 |
Human Methylome Service The Human Methylome is a Next-Generation Sequencing (NGS) service assay that allows a comprehensive DNA methylation profiling in human samples from soli... |
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| C02020012 |
MethylCap protein The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-bindin... |
100 µg | ||
| C09010012 |
MethylTaq Plus 2X Master Mix MethylTaq Plus 2X Master Mix is a ready-to-use cocktail containing all components required for the amplification of bisulfite converted NGS libraries, e... |
135 μL | $125.00 | |
| C02030042 |
Premium Methyl UDI-UMI Adapters Module - 96 rxns Premium Methyl UDI-UMI Adapters Module - 96 rxns includes 96 methylated full-length adapters with unique dual indexes (UDI) and un... |
96 rxns | $950.00 | |
| C02030040 |
Premium Methyl UDI-UMI Adapters Module - Set A Premium Methyl UDI-UMI Adapters Module - Set A includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mo... |
24 rxns | $250.00 | |
| C02030041 |
Premium Methyl UDI-UMI Adapters Module - Set B Premium Methyl UDI-UMI Adapters Module - Set B includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mo... |
24 rxns | $250.00 |