Diagenodeは、DNAメチル化の分野の経験者、未経験者どちらの方でも、実験の一貫性のあるデータを確保しながら、できるだけシンプルかつ便利な実験にするために必要なすべてをご提供いたします。Diagenodeは、超音波装置、試薬キット、高品質の抗体、ハイスループットの自動化機能を備えており、特定のDNAメチル化解析のすべての要件に対応します。
DNAメチル化は最初に発見されたエピジェネティクスマークであり、エピジェネティクススにおいて最も広く研究されているトピックです。生体内でDNAは複製後にメチル化され、刷り込みされた遺伝子、X染色体不活性化および癌細胞における腫瘍抑制遺伝子サイレンシングの調節を含む多くの生物学的過程に関与します。．メチル化は、多くの場合プロモーター領域の上流にあるDNAのシトシン - グアニンが豊富な領域（CpGアイランド）で発生します。
DNAメチル化を検討する主な三つのアプローチの概要
| Description | Features | |
|---|---|---|
| Bisulfite conversion | Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution. |
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| Methylated DNA enrichment | (Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA. |
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| Restriction enzyme-based digestion | Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites. |
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| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02010031 |
hMeDIP kit x16 (monoclonal mouse antibody) このキットは、5-ヒドロキシメチルシトシン（5-hmC）(20µl, 50µl, 100µl)に対する最初で唯一のモノクローナル抗体を用いたDNA免疫沈降実験専用に設計されています。キットには、免疫沈降の効率を評価するコントロール配列（5-hmC、5-mCお... |
16 rxns | $665.00 | |
| C02010021 |
MagMeDIP qPCR kit Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo... |
48 rxns (IP) | $710.00 | |
| C02020010 |
MethylCap kit MethylCapキットを用いたアッセイは、ヒトMeCP2タンパク質のメチルCpG結合ドメイン（MBD）を使用したメチル化DNAのアフィニティー精製に基づき、メチル化されたCpGを含むDNAフラグメントを特異的にキャプチャーします。手順は手動プロセスまたはIP-Star®コンパクト自動システムの両方で行え... |
48 rxns | $665.00 | |
| C02030030 |
Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate... |
50 rxns | $225.00 | |
| C02030032 |
Premium Reduced Representation Bisulfite Sequencing (RRBS) kit Reduced Representation Bisulfite Sequencing (RRBS)は単一ヌクレオチドレベルで、全ゲノムバイサルファイトシーケンシングに関連するDNAメチル化を効率的また、低コストで分析する非常に有効的な方法です。 制限MspI酵素（CCGG標的部位）およびサイズ選択を用いてゲノム... |
24 rxns | ||
| C02030033 |
Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit Reduced Representation Bisulfite Sequencing (RRBS)は単一ヌクレオチドレベルで、全ゲノムバイサルファイトシーケンシングに関連するDNAメチル化を効率的また、低コストで分析する非常に有効的な方法です。 制限MspI酵素（CCGG標的部位）およびサイズ選択を用いてゲノム... |
96 rxns | ||
| C02030036 |
Premium RRBS kit V2 (Coming soon) Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, targeted solution to perform genome-scale DNA methylation analysis... |
24 rxns | $1,525.00 | |
| C02030037 |
Premium RRBS kit V2 x96 RRBS for low DNA amounts and accurate analysis Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the s... |
96 rxns | $3,550.00 | |
| C02010034 |
Auto hMeDIP kit x16 (monoclonal mouse antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy... |
16 rxns | $665.00 | |
| C02030031 |
Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate... |
40 rxns | $225.00 | |
| C02030035 |
Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S... |
8 rxns | $115.00 | |
| C02040018 |
DNA hydroxymethylation control package The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer pair. The control can be ad... |
48 rxns | $145.00 | |
| C02040012 |
DNA Methylation control package The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your... |
40 rxns | $285.00 | |
| C02040019 |
DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes... |
48 rxns | $260.00 | |
| G02180000 |
Human Methylome Service NEW! Human Methylome enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker... |
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| C02020012 |
MethylCap protein メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）から構成されています。*このキットで溶解およびせん断することがで... |
100 µg | $540.00 | |
| C09010012 |
MethylTaq Plus 2X Master Mix MethylTaq Plus 2X Master Mix is a ready-to-use cocktail containing all components required for the amplification of bisulfiteconvertedNGS libraries, except p... |
135 μL | $115.00 | |
| C02030040 |
Premium Methyl UMI-UDI Adapters - Set A (Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ... |
24 rxns | $240.00 | |
| C02030041 |
Premium Methyl UMI-UDI Adapters - Set B (Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ... |
24 rxns | $240.00 |
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