Diagenode

エピジェネティクス・DNAメチル化

メチル化DNA結合タンパク質

The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs.

Diagenode’s MethylCap® kit enables high enrichment of double-stranded DNA and a differential fractionation in function of the methylated CpG density. Fractionation reduces the complexity of samples and makes subsequent next generation sequencing easier. Prior to the MethylCap assay, DNA is first extracted and sheared using the Bioruptor® Sonicator.

Overview

メチル化DNA結合タンパク質サービスの推奨・関連製品

  • some alt
    B03000002
    IP-Star® Compact Automated System
  • Methylation kit icon
    C02020010
    MethylCap kit
  • Bioruptor-pico-next-gen-sequencing
    B01060010
    Picoruptor ® Sonicator
  • Methylation kit icon
    C02030032
    Premium Reduced Representation Bisulfite Sequen...
  • Methylation kit icon
    C02030034
    Premium Whole Genome Bisulfite Sequencing (WGBS...
  • Methylation kit icon
    C02020011
    Auto MethylCap kit

イベント

  • Stem Cell Symposium
    Cambridge
    Sep 19-Sep 21, 2018
  • Livestock Genomics IV
    Wellcome Genome Campus, Hinxton, Cambridge, UK
    Sep 21-Sep 22, 2018
 See all events

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics