hMeDIPの場合、メチル化DNA IP(MeDIP)は5-メチルシトシン(5-mC)または5-ヒドロキシメチルシトシン(5-hmC)に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。


メチルDNA IPは以下のようにして実施されます。(要約):培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、IP化されたメチル化DNAは、qPCR、増幅、マイクロアレイ上のハイブリダイゼーションまたは次世代シーケンシングなど、その次の分析に対応準備が完了します。



Cat. No.ProductFormatPrice
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
このキットは、5-ヒドロキシメチルシトシン(5-hmC)(20µl, 50µl, 100µl)に対する最初で唯一のモノクローナル抗体を用いたDNA免疫沈降実験専用に設計されています。キットには、免疫沈降の効率を評価するコントロール配列(5-hmC、5-mCお...
16 rxns $665.00
C02010021 MagMeDIP qPCR kit
Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo...
48 rxns (IP) $710.00
C02010041 MagMeDIP kit
Perform MeDIP-seq experiments, i.e. methylated DNA immunoprecipitation followed by next generation sequencing, to obtain region-resolutio...
10 rxns
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc $45.00
C15410205-50 5-hydroxymethylcytosine (5-hmC) polyclonal antibody (rabbit)
Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.
50 μg $360.00
C15200006-100 5-methylcytosine (5-mC) monoclonal antibody cl. b
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine.
100 µg $430.00
C15200081-100 5-methylcytosine (5-mC) monoclonal antibody 33D3
DNAメチル化分析に使われるこの5-メチルシトシン抗体(クローン33D3)は、最も使用されている抗体です。 またメチル化DNA免疫沈降(MeDIP-seq、MeDIP-on-chip)、免疫染色又は ドットブロットにおいて検証されています。 Diagenodeは、世界中で唯一本物の33D3クローンの独占販売元です!
100 µg $545.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $665.00
C02010014 Auto MagMeDIP kit
Perform Automated MeDIP (seq) (Methylated DNA Immunoprecipitation sequencing) with Diagenode´s Auto MagMeDIP kit to estimate DNA methylation status of ...
48 rxns
C02040018 DNA hydroxymethylation control package
The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer pair. The control can be ad...
48 rxns $145.00
C02040012 DNA Methylation control package
The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your...
40 rxns $285.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...
48 rxns $260.00
C15400001-15 Mouse IgG
The negative Ctrl IgG from mouse has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses ...
15 µg/15 µl $55.00
C15410206 Rabbit IgG
The negative Ctrl IgG from rabbit has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses...
250 µg/250 µl $85.00
C15420001 Rat IgG
The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in ser...
25 µg/ 25 µl $85.00


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    Dec 9-Dec 12, 2023



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