Diagenode

>>>   Click for Diagenode’s approach to COVID-19

DNA Methylation

Methylated DNA immunoprecipitation

Sensitive tumour detection and classification using plasma cell-free DNA methylomes
Read the publication

Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA
Read the method

The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

How it works

In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

Overview

Recommended products for Methylated DNA immunoprecipitation

Cat. No.ProductFormatPrice
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
The hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis....
16 rxns $546.00
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact benchtop automation system is the latest innovation in epigenetics sample preparation automation, replacing the numerous manual, err...
1 unit
C02010021 MagMeDIP qPCR Kit
Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo...
48 rxns $658.00
C02010040 MagMeDIP-seq Package
Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methy...
10 rxns $1,392.00
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc $41.00
C15410205-50 5-hydroxymethylcytosine (5-hmC) polyclonal antibody (rabbit)
Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.
50 μg $332.00
C15200006-100 5-methylcytosine (5-mC) Antibody - cl. b
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine.
100 µg $398.00
C15200081-100 5-methylcytosine (5-mC) Antibody - clone 33D3
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine (33D3 clone).
100 µg $505.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation anal...
16 rxns $546.00
C02010014 Auto MagMeDIP qPCR Kit
Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy...
48 rxns $658.00
C02040018 DNA hydroxymethylation control package
The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer set that can be added to the DNA...
48 rxns $203.00
C02040012 DNA methylation control package
The DNA methylation control package includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer se...
40 rxns $270.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets that...
48 rxns $270.00
C11000002 Hydroxymethyl dCTP
Hydroxymethyl dCTP can be used as a substrate for MethylTaq DNA polymerase (Cat. No. AF-103-0250) permitting the amplification of DNA containi...
250 µl
C15400001-15 Mouse IgG (sample size)
The negative Ctrl IgG from mouse has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses ...
15 µg/15 µl $51.00
C15410206 Rabbit IgG
The negative Ctrl IgG from rabbit has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses...
250 µg/250 µl $77.00
C15420001 Rat IgG
The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in ser...
25 µg/ 25 µl $77.00

Events

 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics