Diagenode

DNA Methylation

Methylated DNA immunoprecipitation

Sensitive tumour detection and classification using plasma cell-free DNA methylomes
Read the publication

Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA
Read the method

The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

How it works

In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

Applications

Advantages

  • Unaffected DNA
  • High enrichment yield
  • Robust & reproducible techniques
  • NGS compatible

Recommended products for Methylated DNA immunoprecipitation

Cat. No.ProductFormatPrice
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
The hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysi...
16 rxns $690.00
C02010021 MagMeDIP Kit
  Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries f...
48 rxns (IP) $750.00
C02010041 MagMeDIP kit
Perform MeDIP-seq experiments, i.e. methylated DNA immunoprecipitation followed by next generation sequencing, to obtain region-resolutio...
10 rxns
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc $45.00
C15410205-50 5-hydroxymethylcytosine (5-hmC) Antibody (rabbit)
Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.
50 μg $380.00
C15200006-100 5-methylcytosine (5-mC) Antibody - cl. b
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine.
100 µg $450.00
C15200081-100 5-methylcytosine (5-mC) Antibody - clone 33D3
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine (33D3 clone).
100 µg $575.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation anal...
16 rxns $690.00
C02010014 Auto MagMeDIP qPCR Kit - ordering reference: C02010021
The reference C02010014 has been replaced by C02010021.   Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-S...
48 rxns
C02040018 DNA hydroxymethylation control package
The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer set that can be added to the DNA...
48 rxns $150.00
C02040012 DNA methylation control package
The DNA methylation control package includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer ...
40 rxns $300.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets th...
48 rxns $275.00
C15400001-15 Mouse IgG (sample size)
The negative Ctrl IgG from mouse has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses ...
15 µg/15 µl $75.00
C15410206 Rabbit IgG
The negative Ctrl IgG from rabbit has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses...
250 µg/250 µl $85.00
C15420001 Rat IgG
The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in ser...
25 µg/ 25 µl $90.00

Events

  • EpiPlant 2024
    Clermont-Ferrand, France
    Jul 10-Jul 12, 2024
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