Diagenode

DNA Methylation

Methylated DNA immunoprecipitation

The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

How it works

In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

Overview

Recommended products for Methylated DNA immunoprecipitation

Cat. No.ProductFormatPrice
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $510.00
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact benchtop automation system is the latest innovation in epigenetics sample preparation automation, replacing the numerous manual, err...
1 unit
C02010021 MagMeDIP qPCR Kit
Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo...
48 rxns $615.00
C02010040 MagMeDIP-seq Package
*Use the MAGPACK02 promo code in your online order or by clicking on the banner. Sensitive tumour detection and classification using plasma cel...
10 rxns $1,299.00
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc $40.00
C15410205-50 5-hydroxymethylcytosine (5-hmC) polyclonal antibody (rabbit) - Classic
Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.
50 μg $315.00
C15200081-100 5-methylcytosine (5-mC) monoclonal antibody 33D3 - Premium
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine (33D3 clone).
100 µg $485.00
C15200006-100 5-methylcytosine (5-mC) monoclonal antibody cl. b - Classic
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine.
100 µg/48 µl $380.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $510.00
C02010014 Auto MagMeDIP qPCR Kit
Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy...
48 rxns $615.00
C02010011 Auto MeDIP kit - New reference C02010013
Please refer to Auto MagMeDIP kit Perform Automated MeDIP (seq) (Methylated DNA Immunoprecipitation sequencing) to estimate DNA methylation status of your s...
16 rxns
C02040012 DNA Methylation control package
The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your...
40 rxns $250.00
C02010030 hMeDIP kit x16 (monoclonal rat antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $510.00
C02010032 hMeDIP kit x16 (polyclonal rabbit antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $510.00
C11000002 Hydroxymethyl dCTP
Hydroxymethyl dCTP can be used as a substrate for MethylTaq DNA polymerase (Cat. No. AF-103-0250) permitting the amplification of DNA containi...
250 µl $510.00
C15400001-15 Mouse IgG
The negative Ctrl IgG from mouse has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses ...
15 µg/15 µl $45.00
C15410206 Rabbit IgG
The negative Ctrl IgG from rabbit has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses...
250 µg/250 µl $65.00
C15420001 Rat IgG
The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in ser...
25 µg/ 25 µl $70.00

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