Rat IgG

Catalog Number
25 µg/ 25 µl
  Bulk order

The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in hMeDIP experiments (Cat. No. AF-104-0016) as a negative control in parallel with the specific monoclonal 5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100). Both antibodies should be used at the same concentration.

Concentration1.0 µg/µl
Species reactivitynone
PurityProtein A purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
  • Validation data


    Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody
    hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. The DNA was spiked with 0.025 ng of each of the DNA standards from the 5-hmC, 5-mC & cytosine DNA standard pack (cat. No. C02040011), prior to the IP Rat IgG (cat. No. C15420001) was used as a negative IP control. 2.5 μg of antibody per ChIP experiment was used for both antibodies. Quantitative PCR was performed with the primers specific for the unmethylated, methylated and hydroxymethylated controls present in the kit. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  •  Applications
    Methylated DNA immunoprecipitation
    Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNARead the method The Methylated DNA Immunoprec... Read more
  •  Documents
    Datasheet ratIgG AF-105-0025 DATASHEET
    Datasheet description
  •  Safety sheets
    Rat IgG SDS GB en Download
    Rat IgG SDS US en Download
    Rat IgG SDS BE nl Download
    Rat IgG SDS BE fr Download
    Rat IgG SDS FR fr Download
    Rat IgG SDS ES es Download
    Rat IgG SDS DE de Download
    Rat IgG SDS JP ja Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Rat IgG (Diagenode Cat# C15420001 Lot# D001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
    Komar DN, Mouriz A, Jarillo JA, Piñeiro M
    Intricate gene regulatory networks orchestrate biological processes and developmental transitions in plants. Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expr...

  •  Related products


  • London Calling 2024
    London, UK
    May 21-May 24, 2024
  • Symposium of the Young Scientist Association
    Vienna, Austria
    May 28-May 29, 2024
  • ESHG 2024
    Berlin, Germany
    Jun 1-Jun 4, 2024
  • CLEPIC 2024
    Warsaw, Poland
    Jun 5-Jun 7, 2024
  • EACR 2024
    Rotterdam, Netherlands
    Jun 10-Jun 13, 2024
  • Chromatin meets South 2024
    Marseille, France
    Jun 13-Jun 14, 2024
 See all events


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics