Rat IgG

25 µg/ 25 µl
  Bulk order

The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in hMeDIP experiments (Cat. No. AF-104-0016) as a negative control in parallel with the specific monoclonal 5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100). Both antibodies should be used at the same concentration.

Concentration1.0 µg/µl
Species reactivitynone
PurityProtein A purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
  • Validation data


    Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody
    hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. The DNA was spiked with 0.025 ng of each of the DNA standards from the 5-hmC, 5-mC & cytosine DNA standard pack (cat. No. C02040011), prior to the IP Rat IgG (cat. No. C15420001) was used as a negative IP control. 2.5 μg of antibody per ChIP experiment was used for both antibodies. Quantitative PCR was performed with the primers specific for the unmethylated, methylated and hydroxymethylated controls present in the kit. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  •  应用
    Methylated DNA immunoprecipitation
    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP i... Read more
  •  文档
    Datasheet ratIgG AF-105-0025 DATASHEET
    Datasheet description
  •  Safety sheets
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  •  出版物

    How to properly cite this product in your work

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    Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
    Komar DN, Mouriz A, Jarillo JA, Piñeiro M
    Intricate gene regulatory networks orchestrate biological processes and developmental transitions in plants. Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expr...

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