Rat IgG

Catalog Number
25 µg/ 25 µl
  Bulk order

The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in hMeDIP experiments (Cat. No. AF-104-0016) as a negative control in parallel with the specific monoclonal 5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100). Both antibodies should be used at the same concentration.

Concentration1.0 µg/µl
Species reactivitynone
PurityProtein A purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
  • Validation data


    Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody
    hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. The DNA was spiked with 0.025 ng of each of the DNA standards from the 5-hmC, 5-mC & cytosine DNA standard pack (cat. No. C02040011), prior to the IP Rat IgG (cat. No. C15420001) was used as a negative IP control. 2.5 μg of antibody per ChIP experiment was used for both antibodies. Quantitative PCR was performed with the primers specific for the unmethylated, methylated and hydroxymethylated controls present in the kit. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  •  実験手法
    hMeDIPの場合、メチル化DNA IP(MeDIP)は5-メチルシトシン(5-mC)または5-ヒドロキシメチルシトシン(5-hmC)に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。 使い方 メチルDNA IPは以下のようにして実施されます。(要約):培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、... Read more
  •  資料
    Datasheet ratIgG AF-105-0025 DATASHEET
    Datasheet description
  •  Safety sheets
    Rat IgG SDS GB en Download
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  •  出版物

    How to properly cite this product in your work

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    Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
    Komar DN, Mouriz A, Jarillo JA, Piñeiro M
    Intricate gene regulatory networks orchestrate biological processes and developmental transitions in plants. Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expr...

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