Diagenode

エピジェネティクス・DNAメチル化

バイサルファイト処理

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.
Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

How it works

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).


Figure 1: Overview of bisulfite conversion of DNA

Recommended products for バイサルファイト処理

Cat. No.ProductFormatPrice
G02020000 DNA メチル化プロファイリング (RRBS 受託サービス)
RRBSとDNAメチル化研究 最新のバイサルファイト処理とIlluminaシーケンシング技術 CpGメチル化解析による単一ヌクレオチド分解能 出版品質のデータ 人間、動物、植物のゲノム RRBS(Reduced representation bisulfite s...
C02030030 Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
50 rxns $200.00
C02030032 Premium Reduced Representation Bisulfite Sequencing (RRBS) kit
Reduced Representation Bisulfite Sequencing (RRBS)は単一ヌクレオチドレベルで、全ゲノムバイサルファイトシーケンシングに関連するDNAメチル化を効率的また、低コストで分析する非常に有効的な方法です。 制限MspI酵素(CCGG標的部位)およびサイズ選択を用いてゲノム...
24 rxns $1,455.00
C05010033 Premium WGBS Indexes - 12
Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
96 rxns $1,120.00
C05010034 Premium WGBS Indexes - 24
Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
192 rxns $2,015.00
C05010032 Premium WGBS Indexes - 6
Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...
48 rxns $655.00
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
Premium Whole Genome Bisulfite Sequencing(WGBS)キットは、イルミナ®プラットフォームを使用したシーケンシングのシングルおよびペアエンドのバイサルファイト変換DNAライブラリー調製専用に設計されています。 ChIP-Bis-Sequencingを実施するために、...
8 rxns $745.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $200.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $99.00
C02030021 MethylEasy™ kit Xceed 40rxns
Diagenode’s MethylEasy allows for the detection of every methylated Cytosine of your target sequences.
40 rxns $245.00

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