Bisulfite sequencing(Bis-Seq) - Bisulfite conversion - DNA Methylation

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.

Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

How it works

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).

Figure 1: Overview of bisulfite conversion of DNA

バイサルファイト処理サービスの推奨・関連製品

Cat. No.	Product	Format	Price
B03000002	SX-8G IP-Star^® Compact Automated System IP-Star®Compactベンチトップオートメーションシステムは、エピジェネティクスサンプル事前調製の自動化革命の中での最新の技術であり、複雑なエピジェネティクスプロトコール及びエラーが発生しやすくなる手動でのライブラリー調製アプリケーションに代わるものです。このシステムは手動とは異なり、作動中の介...	1 unit		Get a quote You are about to request a quote for SX-8G IP-Star^® Compact Automated System. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
C02030030	Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...	50 rxns	$210.00
C02030032	Premium Reduced Representation Bisulfite Sequencing (RRBS) kit Reduced Representation Bisulfite Sequencing (RRBS)は単一ヌクレオチドレベルで、全ゲノムバイサルファイトシーケンシングに関連するDNAメチル化を効率的また、低コストで分析する非常に有効的な方法です。制限MspI酵素（CCGG標的部位）およびサイズ選択を用いてゲノム...	24 rxns	$1,530.00
C02030033	Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...	96 rxns	$4,095.00
C02030034	Premium Whole Genome Bisulfite Sequencing (WGBS) kit Premium Whole Genome Bisulfite Sequencing（WGBS）キットは、イルミナ®プラットフォームを使用したシーケンシングのシングルおよびペアエンドのバイサルファイト変換DNAライブラリー調製専用に設計されています。 ChIP-Bis-Sequencingを実施するために、...	8 rxns	$780.00
C02030031	Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...	40 rxns	$210.00
C02030035	Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...	8 rxns	$105.00
C05010033	Premium WGBS Indexes - 12 Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...	96 rxns	$1,175.00
C05010034	Premium WGBS Indexes - 24 Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...	192 rxns	$2,115.00
C05010032	Premium WGBS Indexes - 6 Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...	48 rxns	$690.00

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エピジェネティクス・DNAメチル化

バイサルファイト処理

How it works

バイサルファイト処理サービスの推奨・関連製品

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