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DNA Methylation

Bisulfite conversion

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.
Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

How it works

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).


Figure 1: Overview of bisulfite conversion of DNA

针对 Bisulfite conversion 的推荐产品

Cat. No.ProductFormatPrice
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact benchtop automation system is the latest innovation in epigenetics sample preparation automation, replacing the numerous manual, err...
1 unit
C02030030 Premium Bisulfite kit
Make your Bisulfite conversion now in only 90 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r...
50 rxns $214.00
C02030032 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...
24 rxns $1,561.00
C02030033 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...
96 rxns $4,177.00
C02030036 Premium RRBS kit V2
(Coming soon) Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, targeted solution to perform genome-scale DNA methylation analysis...
24 rxns
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes Compatible with ChIP-Bis-Seq - Com...
8 rxns $796.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $214.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $107.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...
48 rxns $270.00
C02030040 Premium Methyl UMI-UDI Adapters - Set A
(Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ...
24 rxns
C02030041 Premium Methyl UMI-UDI Adapters - Set B
(Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ...
24 rxns
C05010033 Premium WGBS Indexes - 12
Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
96 rxns $1,199.00
C05010034 Premium WGBS Indexes - 24
Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
192 rxns $2,157.00
C05010032 Premium WGBS Indexes - 6
Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...
48 rxns $704.00

活动

  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
  • Dutch Chromatin Meeting
    Radboud UMC, Geert Grooteplein 15, 6525 EZ Nijmegen, The Netherlands, Experience center, route 292 to 307
    Nov 12, 2021
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