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DNA Methylation

Bisulfite conversion

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.

Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

How it works

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).

Figure 1: Overview of bisulfite conversion of DNA

针对 Bisulfite conversion 的推荐产品

Cat. No.	Product	Format	Price
B03000002	IP-Star^® Compact Automated System The IP-Star® Compact benchtop automation system is the latest innovation in epigenetics sample preparation automation, replacing the numerous manual, err...	1 unit		Get a quote You are about to request a quote for IP-Star^® Compact Automated System. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
C02030030	Premium Bisulfite kit Make your Bisulfite conversion now in only 90 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r...	50 rxns	$214.00
C02030032	Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...	24 rxns	$1,561.00
C02030033	Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...	96 rxns	$4,177.00
C02030036	Premium RRBS kit V2 (Coming soon) Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, targeted solution to perform genome-scale DNA methylation analysis...	24 rxns		Get a quote You are about to request a quote for Premium RRBS kit V2. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
C02030034	Premium Whole Genome Bisulfite Sequencing (WGBS) kit Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes Compatible with ChIP-Bis-Seq - Com...	8 rxns	$796.00
C02030031	Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...	40 rxns	$214.00
C02030035	Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...	8 rxns	$107.00
C02040019	DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...	48 rxns	$270.00
C02030040	Premium Methyl UMI-UDI Adapters - Set A (Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ...	24 rxns		Get a quote You are about to request a quote for Premium Methyl UMI-UDI Adapters - Set A. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
C02030041	Premium Methyl UMI-UDI Adapters - Set B (Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ...	24 rxns		Get a quote You are about to request a quote for Premium Methyl UMI-UDI Adapters - Set B. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
C05010033	Premium WGBS Indexes - 12 Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...	96 rxns	$1,199.00
C05010034	Premium WGBS Indexes - 24 Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...	192 rxns	$2,157.00
C05010032	Premium WGBS Indexes - 6 Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...	48 rxns	$704.00

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DNA methylation workshop: Become an expert at RRBS
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Radboud UMC, Geert Grooteplein 15, 6525 EZ Nijmegen, The Netherlands, Experience center, route 292 to 307
Nov 12, 2021

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