Diagenode

Epigenetics DNA Methylation

Bisulfite conversion

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.
Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

How it works

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).


Figure 1: Overview of bisulfite conversion of DNA

Made for Bisulfite conversion

Cat. No.ProductFormatPrice
G02020000 DNA Methylation Profiling (RRBS Service)
DNA methylation studies with RRBS The latest bisulfite conversion and Illumina sequencing technologies Single nucleotide resolution through CpG met...
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit
C02030030 Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
50 rxns VAR pricing available
C02030032 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...
24 rxns VAR pricing available
C05010033 Premium WGBS Indexes - 12
Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
96 rxns VAR pricing available
C05010034 Premium WGBS Indexes - 24
Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
192 rxns VAR pricing available
C05010032 Premium WGBS Indexes - 6
Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...
48 rxns VAR pricing available
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using ...
8 rxns VAR pricing available
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns VAR pricing available
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns VAR pricing available
C02030021 MethylEasy™ kit Xceed 40rxns
Diagenode’s MethylEasy allows for the detection of every methylated Cytosine of your target sequences.
40 rxns VAR pricing available

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