Diagenode

DNA Methylation

Bisulfite conversion

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.
Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

How it works

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).


Figure 1: Overview of bisulfite conversion of DNA

Recommended products for Bisulfite conversion

Cat. No.ProductFormatPrice
C02030030 Premium Bisulfite kit
Make your Bisulfite conversion now in only 60 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r...
50 rxns $220.00
C02030032 Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036
The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: Premium RRBS v2 (24 rxns). Please note, that the adapters are not included in the ki...
24 rxns
C02030033 Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037
The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: Premium RRBS v2 (96 rxns). Please note, that the adapters are not included in the ki...
96 rxns
C02030036 Premium RRBS kit V2
RRBS for low DNA amounts and accurate analysis

Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the s...
24 rxns $1,480.00
C02030037 Premium RRBS kit V2 x96
RRBS for low DNA amounts and accurate analysis

Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the s...
96 rxns $3,445.00
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes Compatible with ChIP-Bis-Seq - Com...
8 rxns $810.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $220.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $110.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets that...
48 rxns $250.00
C02030040 Premium Methyl UDI-UMI Adapters Module - Set A
Premium Methyl UDI-UMI Adapters Module - Set A includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mole...
24 rxns $235.00
C02030041 Premium Methyl UDI-UMI Adapters Module - Set B
  Premium Methyl UDI-UMI Adapters Module - Set B includes 24 methylated full-length adapters with unique dual indexes (UDI) and uni...
24 rxns $235.00
C05010033 Premium WGBS Indexes - 12
Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
96 rxns $1,225.00
C05010034 Premium WGBS Indexes - 24
Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
192 rxns $2,200.00
C05010032 Premium WGBS Indexes - 6
Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...
48 rxns $720.00

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