Diagenode

DNA Methylation

Bisulfite conversion

Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.
Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

How it works

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).


Figure 1: Overview of bisulfite conversion of DNA

Recommended products for Bisulfite conversion

Cat. No.ProductFormatPrice
G02020000 DNA Methylation Profiling (RRBS Service)
Superior coverage for DNA methylation studies: fast and affordable Superior coverage– 4 million CpGs High throughput - 96 samples processed in ...
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact benchtop automation system is the latest innovation in epigenetics sample preparation automation, replacing the numerous manual, err...
1 unit
C02030030 Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
50 rxns $200.00
C02030032 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...
24 rxns $1,455.00
C05010033 Premium WGBS Indexes - 12
Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
96 rxns $1,120.00
C05010034 Premium WGBS Indexes - 24
Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
192 rxns $2,015.00
C05010032 Premium WGBS Indexes - 6
Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...
48 rxns $655.00
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using ...
8 rxns $745.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $200.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $99.00
C02030021 MethylEasy™ kit Xceed 40rxns
Diagenode’s MethylEasy allows for the detection of every methylated Cytosine of your target sequences.
40 rxns $245.00

Events

  • Stem Cell Symposium
    Cambridge
    Sep 19-Sep 21, 2018
  • 2018 PacBio User Group Meeting (UGM)
    St Louis
    Sep 19-Sep 20, 2018
  • Livestock Genomics IV
    Wellcome Genome Campus, Hinxton, Cambridge, UK
    Sep 21-Sep 22, 2018
 See all events

Applications

 See all applications



The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics