Diagenode

Next Generation Sequencing

FFPE DNA extraction

Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by traditional methods or can be sheared with the Bioruptor® Pico ultrasonicator for downstream NGS library prep using the MicroPlex Library Preparation Kit.

Recommended products for FFPE DNA extraction

  • Bioruptor Pico
    B01080010
    Bioruptor® Pico sonication device
  • Bioruptor Plus Sonication Device
    B01020001
    Bioruptor® Plus sonication device
  • some alt
    C30010010-300
    1.5 ml Bioruptor® Plus TPX microtubes
  • some alt
    C30010010-50
    1.5 ml Bioruptor® Plus TPX microtubes
  • some alt
    C30010016
    1.5 ml Bioruptor® Pico Microtubes with Caps
  • some alt
    B01200011
    1.5 ml tube holder for Bioruptor® Standard ...
  • Bioruptor pico next gen sequencing
    B01060010
    Bioruptor® Pico sonication device

Related protocols

FFPE DNA extraction protocol using the Bioruptor®
The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, provid...
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Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®
Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene...
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Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing
Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation o...
Download

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