Diagenode

Next Generation Sequencing

FFPE DNA extraction

Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by traditional methods or can be sheared with the Bioruptor® Pico ultrasonicator for downstream NGS library prep using the MicroPlex Library Preparation Kit.

Recommended products for FFPE DNA extraction

  • Bioruptor-pico-next-gen-sequencing
    B01060010
    Bioruptor® Pico sonication device
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    B01020001
    Bioruptor® Plus sonication device
  • Kit icon
    C20000032
    FFPE DNA Extraction Kit V2
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    C30010010-300
    1.5 ml Bioruptor® Plus TPX microtubes
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    C30010010-50
    1.5 ml Bioruptor® Plus TPX microtubes
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    C30010016
    1.5 ml Bioruptor® Pico Microtubes with Caps
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    B01200040
    1.5 ml tube holder for Bioruptor® Pico
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    B01200011
    1.5 ml tube holder for Bioruptor® Standard ...

Related protocols

FFPE DNA extraction protocol using the Bioruptor®
The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, provid...
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Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®
Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene...
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Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing
Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation o...
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Events

  • Genome instability: when RNA meets chromatin
    Roscoff
    Sep 16-Jun 20, 2019
  • Japanese Biochemical Society
    Yokohama
    Sep 18-Sep 20, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    Atlanta, Georgia
    Sep 19, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    RTP, North Carolina
    Sep 20, 2019
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