H3T3pK4me1 polyclonal antibody - Classic

Figure 1. H3T3pK4me1 antibody Immunofluorescence results
Immunofluorescence Microscopy of H3T3pK4me1 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T3pK4me1 is nuclear and chromosomal. Staining: Histone H3T3pK4me1 is expressed in green while the nuclei and aplpha- tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).

Figure 2. H3T3pK4me1 antibody Western blot results
Western Blot of Rabbit H3T3pK4me1 antibody. Lane 1: HeLa histone extracts. Lane 2. NIH 3T3 histone extracts Lane 3. C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at a 1:1,000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

Figure 3. H3T3pK4me1 antibody Dot blot results
Dot Blot of H3T3pK4me1 antibody. Load: 1, 10, and 100 picomoles of the different peptides. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT.