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H4R3me1 Antibody

Catalog Number
Format
Price
C15210022
100 µg
$408.00
  Bulk order



Monoclonal antibody raised in rabbit against the region of histone H4 containing the monomethylated arginine 3 (H4R3me1), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1 µg/µl
Species reactivityHuman: positive. Other species: not tested.
TypeMonoclonal
PurityAffinity purified monoclonal antibody
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 50% glycerol, 1% BSA and 0.09% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Dot Blotting 1:10,000 Fig 1
Western Blotting 1:1,000 Fig 2
Immunofluorescence 1:500 Fig 3
  • Validation Data

    Figure 1. Cross reactivity tests using the Diagenode monoclonal antibody directed against H4R3me1
    To test the cross reactivity of the Diagenode antibody against H3K18ac (cat. No. C15210022), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4R3. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 3 shows a high specificity of the antibody for the modification of interest.

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H4R3me1
    Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H4R3me1 (cat. No. C15210022). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.

    Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H4R3me1
    HeLa cells were stained with the Diagenode antibody against H3K18ac (cat. No. C15210020, red) diluted 1:500. Actin was stained with fluorescein phalladoin (green).

  •  Applications
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    H4R3me1 Antibody Datasheet DATASHEET
    Download
  •  Safety sheets
    H4R3me1 Antibody SDS US en Download
    H4R3me1 Antibody SDS GB en Download
    H4R3me1 Antibody SDS ES es Download
    H4R3me1 Antibody SDS DE de Download
    H4R3me1 Antibody SDS JP ja Download
    H4R3me1 Antibody SDS BE nl Download
    H4R3me1 Antibody SDS BE fr Download
    H4R3me1 Antibody SDS FR fr Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4R3me1 Antibody (Diagenode Cat# C15210022 Lot# 001). Click here to copy to clipboard.

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Events

  • ChIP workshop
    Diagenode
    Sep 21-Sep 22, 2021
  • DNA methylation workshop: Master MeDIP assay
    Diagenode
    Sep 30, 2021
  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
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