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Figure 1. Immunofluorescence Immunofluorescence of H4R3me1 antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4R3me1 is nuclear and chromosomal. Staining: Histone H4R3me1 is expressed in green, nuclei are counterstained with DAPI (blue).
Figure 2. Western Blot Western Blot of H4R3me1 antibody. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:1,000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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