Figure 1. ChIP results obtained with the Diagenode antibody directed against H3R17me2(asym)
ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3R17me2(asym) (cat. No. CS-092-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (cat. No. kch-oneDIP-060), using sheared chromatin from 1.6 million cells per ChIP reaction. A titration of the antibody consisting of 2, 5, 10 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1A: QPCR performed with primers for the GAPDH promoter (cat. No. pp-1001-050) and for exon 2 of the myoglobin gene (cat. No. pp-1006-050). Figure 1B: QPCR performed with primers for the promoter of the active ALDOA gene and for the coding region of the inactive MYOD gene..