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Figure 1. Western blot analysis using the Diagenode antibody directed against H3K9me3 Western blot was performed using histone extracts from HeLa cells (HeLa HE, 20 μg) and the Diagenode antibody against H3K9me3 (Cat. No. pAb-013-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest in indicated with an arrow.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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Histone H3 modifications associated with differentiation and long-term culture of mesenchymal adipose stem cells. Noer A, Lindeman LC, Collas P Long-term culture of mesenchymal stem cells leads to a loss of differentiation capacity, the molecular mechanism of which remains not understood. We show here that expansion of adipose stem cells (ASCs) to late passage (replicative senescence) is associated with promoter-specific and global changes in epigenetic his...
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