Diagenode

H4S1p Antibody

Catalog Number
Format
Price
C15410298
50 μg
$480.00
  Bulk order



Polyclonal antibody raised in rabbit against Histone H4 (p Ser1), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.89 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Figure 1
Immunofluorescence 1:50 Figure 2, 3
Western Blotting 1:1,000 Figure 4
Immunohistochemistry 1:50
Dot Blotting 1:10,000 Figure 5
  • Validation Data

    H4S1p Antibody ChIP Grade

    Figure 1. ChIP
    Chromatin Immunoprecipitation with the H4S1p antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of H4S1p alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.

    H4S1p Antibody validated in Immunofluorescence

    Figure 2. Immunofluorescence
    Immunofluorescence with the H4S1p antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4S1p is nuclear. Staining: Histone H4S1p is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

    H4S1p Antibody validated in Immunofluorescence

    Figure 3. Immunofluorescence
    Immunofluorescence with the H4S1p antibody. Tissue: Nonmitotic and telophase HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4S1p is nuclear. Staining: Histone H4S1p is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

    H4S1p Antibody validated in Western Blot

    Figure 4. Western Blot
    Western Blot with the H4S1p antibody. 30 μg HeLa histone extracts. Primary antibody used at 1:1000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at a 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.

    H4S1p Antibody validated in Dot Blot

    Figure 5. Dot Blot
    Dot Blot with the H4S1p antibody. Lane 1: H4S1 unmodfied. Lane 2: H4S1p. Lane 3: H4S1 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibodyused at 0,1 μg/ml for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  • Target Description

    Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of H4 Ser1 (H4S1p) has been linked to mitosis and DNA repair. This modification is enriched in sites proximal to double stranded brakes, but not those associated with UV damage. Casein kinase II (CK2) phosphorylates H4 Ser1, and it also implicated in regulating the DNA damage response. Furthermore, recruitment of CK2 requires the SIN3/RPD3 histone deacetylase complex.

  •  Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IHC
    Immunohistochemistry Read more
    DB
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H4S1p C15410298 DATASHEET
    Datasheet description
    Download
  •  Publications

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