H3R8me2(sym) Antibody

Catalog Number
50 μg
  Bulk order

Polyclonal antibody raised in rabbit against H3 (sym-dimethyl Arg8), using a KLH-conjugated synthetic peptide.

Concentration1.1 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Figure 1
Western Blotting 2 μg/mL Figure 2, 3
  • Validation Data

    H3R8me2(sym) Antibody ChIP Grade

    Figure 1. H3R8me2(sym) antibody ChIP results
    Chromatin Immunoprecipitation using the H3R8me2(sym) antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(sym) to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by PCR and normalized to total input.

    H3R8me2(sym) Antibody validated in Western Blot

    Figure 2 H3R8me2(sym) antibody western blot results
    Western Blot of H3R8me2(sym) antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    H3R8me2(sym) Antibody validated in Western blot

    Figure 3. H3R8me2(sym) antibody Western blot results
    Western Blot of H3R8me2(sym) antibody. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 μg per lane. Primary antibody incubated at 2 μg/mL overnight at 4°C. Secondary antibody IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

  • Target Description

    Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, methylation of arginine 8 on histone H3 (H3 R8me2s) is associated with transcriptional repression, and modified by PRMT5, but not CARM1.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3R8me2s C15410287 DATASHEET
    Datasheet description
  •  Publications

    How to properly cite this product in your work

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