H3R8me2(sym) polyclonal antibody - Classic

Catalog Number
50 μg
  Bulk order

Polyclonal antibody raised in rabbit against H3 (sym-dimethyl Arg8), using a KLH-conjugated synthetic peptide.

Concentration1.1 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Figure 1
Western Blotting 2 μg/mL Figure 2, 3
  • Validation Data

    ChIP results

    Figure 1. H3R8me2(sym) antibody ChIP results
    Chromatin Immunoprecipitation using the H3R8me2(sym) antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(sym) to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by PCR and normalized to total input.

    Western blot results

    Figure 2 H3R8me2(sym) antibody western blot results
    Western Blot of H3R8me2(sym) antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Western blot results

    Figure 3. H3R8me2(sym) antibody Western blot results
    Western Blot of H3R8me2(sym) antibody. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 μg per lane. Primary antibody incubated at 2 μg/mL overnight at 4°C. Secondary antibody IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3R8me2s C15410287 DATASHEET
    Datasheet description
  •  Publications

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