| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
H3R8me2(sym) polyclonal antibody
Catalog Number
Format
Price
Polyclonal antibody raised in rabbit against H3 (sym-dimethyl Arg8), using a KLH-conjugated synthetic peptide.
| Lot | 003 |
|---|---|
| Concentration | 0.84 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | Figure 1 |
| Western Blotting | 2 μg/mL | Figure 2, 3 |
- Validation Data
Figure 1. H3R8me2(sym) antibody ChIP results
Chromatin immunoprecipitation using the H3R8me2(sym) antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 µg of H3R8me2(sym) antibody to immunoprecipitate DNA from fixed HeLa cells, alongside a no antibody (No Ab) control. DNA was measured by PCR and normalized to total input.
Figure 2. H3R8me2(sym) antibody Western blot results
Western blot of the H3R8me2(sym) antibody. 30 µg of C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 minutes at room temperature (RT). Predicted/observed size: ~15 kDa. Other bands: None.
Figure 3. H3R8me2(sym) antibody Western blot results
Western blot of the H3R8me2(sym) antibody. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 µg per lane. Primary antibody incubated at 2 µg/mL overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 minutes at room temperature (RT). Predicted/observed size: ~15 kDa. Other bands: None. - 出版物
How to properly cite our product/service in your work
We strongly recommend using this: H3R8me2(sym) polyclonal antibody (Hologic Diagenode Cat# C15410287 Lot# 003). Click here to copy to clipboard.
Using our products or services in your publication? Let us know!