H3pan monoclonal antibody can be used as a loading control for nuclear samples to compare the protein expression level between different samples.
Why do you need a loading control?
- To verify that equal sample amount was loaded on the gel
- To confirm that the electrotransfer was done equally across the lines
- To check that the signal detection after antibody incubation is homogenous across the lines
What is the loading control?
The loading control is an antibody against a protein which is highly expressed in certain types of samples. The level of protein stays constant in different conditions and in different organisms. The molecular weights of the protein of interest and loading control are different, allowing differentiation of both proteins as two different bands.