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H3K9me2 Antibody - ChIP Grade

Catalog Number
Format
Price
C15210019
100 µg/ 100 µl
$408.00
  Bulk order



Monoclonal antibody raised in rabbit against the region of histone H3 containing the dimethylated lysine 9 (H3K9me2), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1 µg/µl
Species reactivityHuman: positive
Other species: not tested
TypeMonoclonal
ChIP-grade
PurityAffinity purified monoclonal antibody
HostRabbit
Storage ConditionsStore at -20°C for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 50% glycerol, 1% BSA and 0.09% azide
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 0.5 µg per IP Fig 1
Western Blotting 1:1,000 Fig 2
Immunofluorescence 1:500 Fig 3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.

  • Validation data

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9me2
    ChIP was performed with the Diagenode antibody against H3K9me2 (cat. No. C15210019) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the HBB promoter and the MYOD1 gene, used as positive controls, and for the EIF4A2 and GAPDH promoters, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9me2
    Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9me2 (cat. No. C15210019). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.

    Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9me2
    HeLa cells were stained with the Diagenode antibody against H3K9me2 (cat. No. C15210019, red) diluted 1:500. Actin was stained with fluorescein phalladoin (green).

  •  Applications
    ChIP-seq (ab)
    Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    C15210019 h3k9me2 Datasheet DATASHEET
    Datasheet of h3k9me2 monoclonal antibody
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K9me2 Antibody - ChIP Grade (Diagenode Cat# C15210019 Lot# 001). Click here to copy to clipboard.

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