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Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9me2 ChIP was performed with the Diagenode antibody against H3K9me2 (cat. No. C15210019) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the HBB promoter and the MYOD1 gene, used as positive controls, and for the EIF4A2 and GAPDH promoters, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9me2 Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9me2 (cat. No. C15210019). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.
Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9me2 HeLa cells were stained with the Diagenode antibody against H3K9me2 (cat. No. C15210019, red) diluted 1:500. Actin was stained with fluorescein phalladoin (green).
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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