Diagenode

H3K9me3 Antibody

Catalog Number
Format
Price
C15310013
(CS-013-100)
100 µl
$380.00
  Bulk order



Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 9 (H3K9me3).

Lot001
Concentrationnot determined
Species reactivityHuman, mouse, drosophila
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:750 Fig 1
ChIP 1-5 μl/ChIP Ref 1
  • Validation Data

    H3K9me3 Antibody validated in Western Blot

    Figure 1. Western blot analysis using the Diagenode antibody directed against H3K9me3
    Histone (acid) extracts from HeLa cells (HeLa HE, 20 μg) were analysed by Western blot using the Diagenode antibody against H3K9me3 (cat# CS-013-100) diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K9 is associated with inactive regions.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3K9me3 CS-013-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  Safety sheets
    H3K9me3 antibody SDS GB en Download
    H3K9me3 antibody SDS US en Download
    H3K9me3 antibody SDS DE de Download
    H3K9me3 antibody SDS JP ja Download
    H3K9me3 antibody SDS BE nl Download
    H3K9me3 antibody SDS BE fr Download
    H3K9me3 antibody SDS FR fr Download
    H3K9me3 antibody SDS ES es Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K9me3 Antibody (Diagenode Cat# C15310013 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Alu retrotransposons modulate Nanog expression through dynamic changes in regional chromatin conformation via aryl hydrocarbon receptor.
    González-Rico FJ, Vicente-García C, Fernández A, Muñoz-Santos D, Montoliu L, Morales-Hernández A, Merino JM, Román AC, Fernández-Salguero PM
    Transcriptional repression of Nanog is an important hallmark of stem cell differentiation. Chromatin modifications have been linked to the epigenetic profile of the Nanog gene, but whether chromatin organization actually plays a causal role in Nanog regulation is still unclear. Here, we report that the formation of ...

    Spatiotemporal control of estrogen-responsive transcription in ERα-positive breast cancer cells.
    P-Y Hsu, H-K Hsu, T-H Hsiao, Z Ye, E Wang, A L Profit, I Jatoi, Y Chen, N B Kirma, V X Jin, Z D Sharp and T H-M Huang
    Recruitment of transcription machinery to target promoters for aberrant gene expression has been well studied, but underlying control directed by distant-acting enhancers remains unclear in cancer development. Our previous study demonstrated that distant estrogen response elements (DEREs) located on chromosome 20q13...

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