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Figure 1. Immunofluorescence Immunofluorescence of H4K16ac antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4K16ac is nuclear and chromosomal. Staining: Histone H4K16ac is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Figure 2. Dot Blot Dot Blot of H4K16ac antibody. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: used at a 1:1,000 dilution for 45 min at 4°C.
Figure 3. Western Blot Western Blot of H4K16ac antibody. 30 μg of HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Primary antibody used at 0.2 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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