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Figure 1. Immunofluorescence Immunofluorescence of H4K16ac antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4K16ac is nuclear and chromosomal. Staining: Histone H4K16ac is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Figure 2. Dot Blot Dot Blot of H4K16ac antibody. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: used at a 1:1,000 dilution for 45 min at 4°C.
Figure 3. Western Blot Western Blot of H4K16ac antibody. 30 μg of HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Primary antibody used at 0.2 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.
IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
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WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
Learn more about: Load... Read more
Epigenetics DNA Methylation
Complete solutions for DNA methylation studies
Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data betwee... Read more
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