Diagenode

H4K16ac polyclonal antibody

目录号
格式
价格
C15410300
50 μg
$395.00
  Bulk order



Polyclonal antibody raised in rabbit against Histone H4 (ac Lys16), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1 mg/ml
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Immunofluorescence 1:100 Figure 1
Dot Blotting 1:1,000 Figure 2
Western Blotting 1:1,000 Figure 3
  • Validation Data

    Immunofluorescence

    Figure 1. Immunofluorescence
    Immunofluorescence of H4K16ac antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4K16ac is nuclear and chromosomal. Staining: Histone H4K16ac is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

    Dot Blot

    Figure 2. Dot Blot
    Dot Blot of H4K16ac antibody. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: used at a 1:1,000 dilution for 45 min at 4°C.

    Western Blot

    Figure 3. Western Blot
    Western Blot of H4K16ac antibody. 30 μg of HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Primary antibody used at 0.2 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.

  •  应用
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
  •  文档
    Datasheet H4K16ac C15410300 DATASHEET
    Datasheet description
    Download
  •  出版物

    How to properly cite this product in your work

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