Figure 1 ChIP results obtained with the Diagenode antibody directed against H3K36me1
ChIP assays were performed using human U2OS cells, the Diagenode antibody against H3K36me1 (cat. No. pAb-089-050) and optimized PCR primer pairs for qPCR. ChIP was performed with the “OneDay ChIP” kit (cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells and stringent washing conditions. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the constitutively expressed GAPDH gene and for the inactive gene MYOD. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).