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Figure 1. Western Blot Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 2. Dot Blot Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Figure 3. Dot Blot Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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