H3R8me2(asym) Antibody

Catalog Number
50 μg
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (asym-dimethyl Arg8), using a KLH-conjugated synthetic peptide.

Concentration0.84 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Figure 1
Immunohistochemistry 1:100
Immunofluorescence 1:200 Figure 2
Western Blotting 1:500 Figure 3
  • Validation Data

    H3R8me2(asym) Antibody ChIP Grade

    Figure 1. H3R8me2(asym) antibody ChIP results
    Chromatin Immunoprecipitation of H3R8me2(asym) antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(asym) and 20ul of magnetic beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR, and normalized to the input chromatin.

    H3R8me2(asym) Antibody valiadted in Immunofluorescence

    Figure 2 H3R8me2(asym) antibody Immunofluorescence results
    Immunofluorescence of H3R8me2(asym) antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3R8me2(asym) is nuclear and chromosomal. Staining: H3R8me2(asym) is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

    H3R8me2(asym) Antibody valiadted in Western Blot

    Figure 3. H3R8me2(asym) antibody Western blot results
    Western Blot of H3R8me2(asym) antibody. Lane 1: HeLa Histone extracts. 2. NIH-3T3 extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

  • Target Description

    Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimethylation of H3 Arg8 (H3 R8Me2) is known as a mark of transcriptional repression. The protein arginine methyltransferases PRMT5 and PRMT2 can both methylate Arg8, with PRMT2 specifically methylating in an asymmetric manner. In addition, asymmetric dimethylation of Arg8 inhibits H3K9 methylation by G9a, but symmetric Arg8Me2 does not.

  •  Applications
    Immunohistochemistry Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3R8me2a C15410286 DATASHEET
    Datasheet description
  •  Publications

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