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Figure 1. Immunofluorescence Immunofluorescence using the H3K56me3 antibody. Tissue: HeLa cells during telophase. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K56me3 is nuclear and chromosomal. Staining: Histone H3K56me3 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Figure 2. Immunofluorescence Immunofluorescence using the H3K56me3 antibody. Tissue: HeLa cells during prophase. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K56me3 is nuclear and chromosomal. Staining: Histone H3K56me3 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Figure 3. Western Blot Western Blot using the H3K56me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 4. Dot Blot Dot Blot using the H3K56me3 antibody. Lane 1: H3K56ac. Lane 2: H3K56me1. Lane 3: H3K56me2. Lane 4: H3K56me3. Lane 5: H3K56 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:40 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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