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H3K4me3T6p Antibody - ChIP Grade

Catalog Number
Format
Price
C15410281
50 μg
$388.00
  Bulk order



Polyclonal antibody raised in rabbit against H3 (trimethyl Lys4, p Thr6), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.55 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Fig 1
Immunohistochemistry 1:50
Immunofluorescence 1:50 Fig 2
Western Blotting 1:500 Fig 3, 4
Dot Blotting 1:1,000 Fig 5
  • Validation Data

    H3K4me3T6p Antibody ChIP Grade

    Figure 1. H3K4me3T6p antibody ChIP results
    Chromatin Immunoprecipitation of H3K4me3T6p antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3K4me3T6p antibody alongside a no antibody (No Ab) control, DNA was measured by qPCR and normalized to total input.

    H3K4me3T6p Antibody validated in Immunofluorescence

    Figure 2. H3K4me3T6p antibody Immunofluorescence results
    Immunofluorescence of H3K4me3T6p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K4me3T6p is nuclear and chromosomal. Staining: H3K4me3T6p is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

    H3K4me3T6p Antibody validated in Western Blot

    Figure 3. H3K4me3T6p antibody Western blot results
    Western Blot of Rabbit H3K4me3T6p antibody. 30 μg C. elegans embryo lysate. Primary antibody at 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    H3K4me3T6p Antibody validated in Western Blot

    Figure 4. H3K4me3T6p antibody Western blot results
    Western Blot of H3K4me3T6p antibody. 30 μg HeLa histone extracts. Primary antibody at 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    H3K4me3T6p Antibody validated in Dot Blot

    Figure 5. H3K4me3T6p antibody Dot blot results
    Dot Blot of Rabbit H3K4me3T6p antibody. Lane 1: Unmodified. Lane 2: T6p. Lane 3: K4Me1T6p. Lane 4: K4Me2T6p. Lane 5: K4Me3T6p. Load: 3, 1, and 0.3 picomoles of peptide. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  • Target Description

    Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, trimethylation of lysine 4 on H3 (H3K4Me3) is a well known mark of gene activation. However, the role of phosphorylation at threonine 6 on H3 (H3T6p) is more obscure. Yet recently, the two modifications have been shown to interact with each other. When H3T6 is phosphorylated by protein kinase C beta 1 (PRKCbeta), the histone demethylase LSD1 is prevented from removing methyl groups from H3K4. This same study also correlated high levels of T6p and PRKCbeta as a possible marker for prostate cancer, as well as tumor progression in xenografts.

  •  Applications
    IHC
    Immunohistochemistry Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3K4me3T6p C15410281 DATASHEET
    Datasheet description
    Download
  •  Publications

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