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H3T11p Antibody - ChIP Grade

Catalog Number
Format
Price
C15410309
50 μg
$325.00
  Bulk order
Other format



Polyclonal antibody raised in rabbit against the region of histone H3 containing the phosphorylated Threonine 11 (H3T11p), using a KLH-conjugated synthetic peptide.

LotA2288P
Concentration1.4 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μg/ChIP Figure 1
ELISA 1:1,000 Figure 2
Dot Blotting 1:20,000 Figure 3
Western Blotting 1:1,000 Figure 4
Immunofluorescence 1:200 Figure 5

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 10 µg per IP.

  • Validation Data

    H3T11p Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against H3T11p
    ChIP assays were performed using human HeLa cells, treated with colcemid, the Diagenode antibody against H3T11p (cat. No. C15410309) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    H3T11p Antibody ELISA validation

    Figure 2. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3T11p (cat. No. C15410309) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:25,700.

    H3T11p Antibody valiadted in Dot Blot

    Figure 3. Cross reactivity tests using the Diagenode antibody directed against H3T11p
    To test the cross reactivity of the Diagenode antibody against H3T11p (cat. No. C15410309), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H3T11. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the modification of interest.

    H3T11p Antibody valiadted in Western Blot

    Figure 4. Western blot analysis using the Diagenode antibody directed against H3T11p
    Western blot was performed on whole cell extracts from untreated HeLa cells (25 μg, lane 1), on histone extracts from HeLa cells treated with colcemid (15 μg, lane 2), and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Diagenode antibody against H3T11p (cat. No. C15410309). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

    H3T11p Antibody valiadted in Immunofluorescence

    Figure 5. Immunofluorescence using the Diagenode antibody directed against H3T11p
    HeLa cells were treated with colcemid and stained with the Diagenode antibody against H3T11p (cat. No. C15410309) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3T11p antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2A, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

  •  Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3T11p C15410309 DATASHEET
    Datasheet description
    Download
  •  Publications

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