H2Bs14p monoclonal antibody - Pioneer

Catalog Number
50 μg/50 μl
  Bulk order

Monoclonal antibody raised in mouse against histone H2B containing the phosphorylated Serine 14 (H2BS14p), using a KLH-conjugated synthetic peptide.

Concentration1.0 µg/µl
Species reactivityHuman
PurityProtein A purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 Fig 1
Dot Blotting 1:2,000 Fig 2
Western Blotting 1:200 Fig 3
  • Validation Data

    Dot blot

    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H2BS14p (Cat. No. MAb-186-050).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.


    Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody against H2BS14p
    To test the cross reactivity of the Diagenode antibody against H2BS14p (Cat. No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    Western blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p
    HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. No. MAb-186-050) diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Dot blotting Read more
  • Documents
    Datasheet H2BS14p MAb-186-050 DATASHEET
    Datasheet description
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H2Bs14p monoclonal antibody - Pioneer (Diagenode Cat# C15200186 Lot# 001). Click here to copy to clipboard.

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