Diagenode

H3K27me3S28p polyclonal antibody - Classic

Catalog Number
Format
Price
C15410293
50 μg
$360.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (trimethyl Lys27, p Ser28), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.66 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP 2-5 μg/million cells Fig 1
Immunofluorescence 1:200 Fig 2, 3
Western Blotting 1:500 Fig 4
Immunochemistry 1:50
Dot Blotting 1:500 - 1:1,000 Fig 5
  • Validation Data

    ChIP

    Figure 1. ChIP
    Chromatin Immunoprecipitation using the H3K27me3S28p antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 μg of H3K27me3S28p and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.

    Immunofluorescence

    Figure 2. Immunofluorescence
    Immunofluorescence of H3K27me3S28p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:200 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K27me3S28p is nuclear and chromosomal. Staining: Histone H3K27me3S28p is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

    Immunofluorescence

    Figure 3. Immunofluorescence
    Immunofluorescence of H3K27me3S28p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:200 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K27me3S28p is nuclear and chromosomal. Staining: Histone H3K27me3S28p is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

    Western Blot

    Figure 4. Western Blot
    Western Blot of H3K27me3S28p antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo cell lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/ Observed size: ~15 kDa. Other band(s): None.

    Dot Blot

    Figure 5. Dot Blot
    Dot Blot of H3K27me3S28p antibody. Lane 1: S28p/K27 unmodified. Lane 2: S28p N-Term. Lane 3: S28p C-term. Lane 4: K27Me3. Lane 5: S28p/K27Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody at 1 μg/ml for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  •  Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H3K27me3S28p C15410293 DATASHEET
    Datasheet description
    Download
  •  Publications

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