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Figure 1. ChIP Chromatin Immunoprecipitation using the H3K27me3S28p antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 μg of H3K27me3S28p and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.
Figure 2. Immunofluorescence Immunofluorescence of H3K27me3S28p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:200 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K27me3S28p is nuclear and chromosomal. Staining: Histone H3K27me3S28p is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Figure 3. Immunofluorescence Immunofluorescence of H3K27me3S28p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:200 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K27me3S28p is nuclear and chromosomal. Staining: Histone H3K27me3S28p is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Figure 4. Western Blot Western Blot of H3K27me3S28p antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo cell lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/ Observed size: ~15 kDa. Other band(s): None.
Figure 5. Dot Blot Dot Blot of H3K27me3S28p antibody. Lane 1: S28p/K27 unmodified. Lane 2: S28p N-Term. Lane 3: S28p C-term. Lane 4: K27Me3. Lane 5: S28p/K27Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody at 1 μg/ml for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
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Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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