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Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H2A.X ChIP assays were performed using HeLa cells, the Diagenode antibody against H2A.X (Cat. No. C15210002) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010051), using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the MYT1 and HBB genes and for the Sat2 satellite repeat, used as positive controls, and for the GAPDH promoter, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H2A.X Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H2A.X (Cat. No. C15210002) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H2A.X HeLa cells were stained with the Diagenode antibody against H2A.X (Cat. No. C15210002, red), diluted 1:1,000. Actin was stained with fluorescein phalladoin (green).
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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