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H2A.X Antibody - ChIP Grade (sample size)

Catalog Number
Format
Price
C15210002-10
10 µg
$95.00
  Bulk order
Other format



Monoclonal antibody raised in rabbit against histone H2A.X using a KLH-conjugated synthetic peptide from the C-terminus of the protein.

Lot001
Concentration1 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityProtein A purified
HostRabbit
PrecautionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Applications Suggested dilution References
ChIP* 0.5 - 1 μg/ChIP Fig 1
Western Blotting 1:1,000 Fig 2
Immunofluorescence 1:1,000 Fig 3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.

  • Validation data

    H2A.X Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H2A.X
    ChIP assays were performed using HeLa cells, the Diagenode antibody against H2A.X (Cat. No. C15210002) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010051), using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the MYT1 and HBB genes and for the Sat2 satellite repeat, used as positive controls, and for the GAPDH promoter, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    H2A.X Antibody validated in Western blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H2A.X
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H2A.X (Cat. No. C15210002) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    H2A.X Antibody validated in Immunofluorescence

    Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H2A.X
    HeLa cells were stained with the Diagenode antibody against H2A.X (Cat. No. C15210002, red), diluted 1:1,000. Actin was stained with fluorescein phalladoin (green).

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. H2A.X is an histone variant which replaces H2A in some nucleosomes.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    H2A.X monoclonal antibody DATASHEET
    Monoclonal antibody raised in rabbit against histone H2A.X using a KLH-conjugated synthetic pepti...
    Download
  •  Publications

    How to properly cite this product in your work

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