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Figure 1. H4K20me2 ChIP results ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG (Cat. No. C15200001) or H4K20me2 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
Figure 2. H4K20me2 Western Blot H4K20me2 antibody detects Histone H4 protein dimethylated at Lys20 by western blot analysis. A. 30 μg HeLa whole cell extract B. 30 μg HeLa nuclear extract H4K20me2 antibody dilution: 1:5,000
Figure 3. H4K20me2 IF results H4K20me2 antibody detects Histone H4 dimethylated at Lys20 at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (dimethylated at Lys20) stained by H4K20me2 antibody diluted 1:500.
IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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Quantifying propagation of DNA methylation and hydroxymethylation withiDEMS. Stewart-Morgan K. R. et al. DNA methylation is a critical epigenetic mark in mammalian cells. Many aspects of DNA methylation maintenance have been characterized; however, the exact kinetics of post-replicative methylation maintenance remain a subject of debate. Here we develop isolation of DNA by 5-ethynyl-deoxyuridine labelling for mass spec...
