H4K20me2 Antibody

Catalog Number
100 µl
  Bulk order

Monoclonal antibody raised in mouse against Histone H4 (di methyl Lys20) (histone cluster 1, H4a), using a KLH-conjugated synthetic peptide.

Concentration1 μg/μl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Figure 1
ICC/IF 1:100 - 1:1,000 Figure 3
Western Blotting 1:1,000 - 1:10,000 Figure 2
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    H4K20me2 Antibody ChIP Grade

    Figure 1. H4K20me2 ChIP results
    ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG (Cat. No. C15200001) or H4K20me2 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

    H4K20me2 Antibody validated in Western Blot

    Figure 2. H4K20me2 Western Blot
    H4K20me2 antibody detects Histone H4 protein dimethylated at Lys20 by western blot analysis. A. 30 μg HeLa whole cell extract B. 30 μg HeLa nuclear extract H4K20me2 antibody dilution: 1:5,000

    H4K20me2 Antibody validated in Immunofluorescent

    Figure 3. H4K20me2 IF results
    H4K20me2 antibody detects Histone H4 dimethylated at Lys20 at nucleus by immunofluorescent analysis.
    Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
    Green: Histone H4 (dimethylated at Lys20) stained by H4K20me2 antibody diluted 1:500.

  •  Applications
    Immunochemistry Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H4K20me2 C15200205 DATASHEET
    Datasheet description
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
  •  Safety sheets
    H4K20me2 antibody SDS US en Download
    H4K20me2 antibody SDS GB en Download
    H4K20me2 antibody SDS BE fr Download
    H4K20me2 antibody SDS FR fr Download
    H4K20me2 antibody SDS ES es Download
    H4K20me2 antibody SDS DE de Download
    H4K20me2 antibody SDS JP ja Download
    H4K20me2 antibody SDS BE nl Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K20me2 Antibody (Diagenode Cat# C15200205 Lot# 41582). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Quantifying propagation of DNA methylation and hydroxymethylation withiDEMS.
    Stewart-Morgan K. R. et al.
    DNA methylation is a critical epigenetic mark in mammalian cells. Many aspects of DNA methylation maintenance have been characterized; however, the exact kinetics of post-replicative methylation maintenance remain a subject of debate. Here we develop isolation of DNA by 5-ethynyl-deoxyuridine labelling for mass spec...

    Genome-wide and sister chromatid-resolved profiling of protein occupancyin replicated chromatin with ChOR-seq and SCAR-seq
    Petryk Nataliya et al.
    Elucidating the mechanisms underlying chromatin maintenance upon genome replication is critical for the understanding of how gene expression programs and cell identity are preserved across cell divisions. Here, we describe two recently developed techniques, chromatin occupancy after replication (ChOR)-seq and sister...

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