Diagenode

Antibodies

Histones

Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A nucleosome consists of one pair of each of the core histones (H2A, H2B, H3 and H4) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone H1, allowing for further condensation of chromatin.

The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome.  They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These post-translational modifications include  methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation.  The most well characterized PTMs are methylation, acetylation and phosphorylation. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).

The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with euchromatin and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in heterochromatin and associated with gene silencing. The combination of different histone modifications is called the "histone code”, analogous to the genetic code.

Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our  antibodies are highly specific and have been validated in many applications, including ChIP and ChIP-seq.

Diagenode’s collection includes antibodies recognizing:

  • Histone H1 variants
  • Histone H2A, H2A variants and histone H2A modifications (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)
  • Histone H2B and H2B modifications (serine phosphorylation, lysine acetylation)
  • Histone H3 and H3 modifications (lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))
  • Histone H4 and H4 modifications (lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )

HDAC's HAT's, HMT's and other enzymes which modify histones can be found in the category Histone modifying enzymes

Diagenode’s highly validated antibodies:

  •         Highly sensitive and specific
  •         Cost-effective (requires less antibody per reaction)
  •         Batch-specific data is available on the website
  •         Expert technical support
  •         Sample sizes available
  •        100% satisfaction guarantee

Products

ProductApplicationPurity
H3R2me2(asym) polyclonal antibody - Classic
(50 μg /50 μl)
ChIP-qPCR WB Affinity purified
H1.0 polyclonal antibody - Pioneer
(100 µl)
ELISA Whole antiserum
H1.1 polyclonal antibody - Pioneer
(100 µl)
ELISA Whole antiserum
H1.3 polyclonal antibody - Pioneer
(100 µl)
ELISA Whole antiserum
H1.4 polyclonal antibody - Pioneer
(100 µl)
ELISA Whole antiserum
H1.5 polyclonal antibody - Pioneer
(100 µl)
ELISA Whole antiserum
H2A.XS139p polyclonal antibody - Classic
(50 μg/ 19 μl)
DB ELISA IF WB Affinity purified
H2A.XS139p polyclonal antibody - Pioneer
(100 µl)
ELISA Whole antiserum
H2A.Z polyclonal antibody - Premium
(50 μg/33 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq ELISA IF WB Affinity purified
H2A.Zac polyclonal antibody - Classic
(50 μg/72 μl)
ChIP-qPCR ChIP-seq DB ELISA IF Affinity purified
H2A.Zac polyclonal antibody - Premium
(50 μg/46 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H2AK119ub polyclonal antibody - Classic
(50 μg/46 μl)
ChIP-qPCR DB ELISA WB Affinity purified
H2AK5ac polyclonal antibody - Classic
(50 μg/ 54 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H2Apan polyclonal antibody - Classic
(50 μg/79 μl)
ChIP-qPCR ELISA IF WB Affinity purified
H2BK12ac polyclonal antibody - Classic
(50 μg/ 61 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H2BK15ac polyclonal antibody - Classic
(50 μg/ 24 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H2Bpan polyclonal antibody - Classic
(50 μg/34 μl)
ChIP-qPCR DB ELISA Affinity purified
H2Bs14p monoclonal antibody - Pioneer
(50 μg/50 μl)
DB ELISA Protein A purified
H3K14ac polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB ELISA WB Affinity purified
H3K18ac polyclonal antibody - Classic
(100 µl)
DB ELISA WB Whole antiserum
H3K18ac polyclonal antibody - Classic
(50 μg/62 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K18me1 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB IF WB Affinity purified
H3K18me2 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB Epigenetics DNA Methylation IF WB Affinity purified
H3K18me3 polyclonal antibody - Classic
(50 μg)
DB Epigenetics DNA Methylation IF WB Affinity purified
H3K23ac polyclonal antibody - Classic
(100 µl)
DB ELISA WB Whole antiserum
H3K27ac monoclonal antibody - Classic
(50 μg/50 μl)
ChIP-qPCR ELISA IF WB Protein A purified
H3K27ac polyclonal antibody - Classic
(50 μg/42 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K27ac polyclonal antibody - Premium
(50 μg/18 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K27me1 polyclonal antibody - Classic
(50 µg/82 µl)
ChIP-qPCR DB ELISA IF WB Affinity purified
H3K27me2 polyclonal antibody - Classic
(50 µg/19 µl)
ChIP-qPCR DB ELISA IF WB Affinity purified
H3K27me2/3 monoclonal antibody - Classic
(50 µg/34 µl)
ChIP-qPCR DB IF WB Protein G purified
H3K27me3 monoclonal antibody - Classic
(50 μg/50 μl)
ChIP-qPCR ELISA IF WB Protein A purified
H3K27me3 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA IF WB Whole antiserum
H3K27me3 polyclonal antibody - Classic
(50 μg/34 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K27me3 polyclonal antibody - Premium
(50 μg/27 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
H3K27me3S28p polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA IF IP WB Whole antiserum
H3K27me3S28p polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB Epigenetics DNA Methylation IF WB Affinity purified
H3K27me3S28p polyclonal antibody - Classic
(50 μg/ 295 μl)
ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
H3K36ac polyclonal antibody - Classic
(50 μg)
ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
H3K36me1 polyclonal antibody - Classic
(50 µg/54 µl)
ChIP-qPCR DB ELISA WB Affinity purified
H3K36me2 monoclonal antibody - Classic
(50 μg/50 μl)
ChIP-qPCR DB ELISA WB Protein A purified
H3K36me2 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Whole antiserum
H3K36me3 monoclonal antibody - Classic
(50 μg/50 μl)
ChIP-qPCR DB ELISA IF WB Protein A purified
H3K36me3 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR ChIP-seq DB ELISA WB Whole antiserum
H3K36me3 polyclonal antibody - Classic
(50 µg/56 µl)
ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
H3K36me3 polyclonal antibody - Premium
(50 μg/42 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K37me1 polyclonal antibody - Classic
(50 μg)
DB WB Affinity purified
H3K4ac polyclonal antibody - Classic
(50 μg/34 μl)
ChIP-qPCR DB ELISA IF WB Affinity purified
H3K4ac polyclonal antibody - Classic
(50 μg/46 μl)
ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
H3K4me1 monoclonal antibody - Classic
(50 µg/50 µl)
ChIP-qPCR ELISA IF WB Protein A purified
H3K4me1 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H3K4me1 polyclonal antibody - Classic
(50 µg/18 µl)
ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
H3K4me1 polyclonal antibody - Premium
(50 μg/54 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
H3K4me1T6p polyclonal antibody - Classic
(50 μg)
DB IF IHC WB Affinity purified
H3K4me2 monoclonal antibody - Classic
(50 µg/50 µl)
ChIP-qPCR ELISA IF WB Protein A purified
H3K4me2 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H3K4me2 polyclonal antibody - Classic
(50 μg/42 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K4me3 monoclonal antibody - Classic
(50 µg/50 µl)
ChIP-qPCR ChIP-seq ELISA IF WB Protein A/G purified
H3K4me3 polyclonal antibody - Classic
(100 µl)
DB WB Whole antiserum
H3K4me3 polyclonal antibody - Classic
(50 µg/25 µl)
ChIP-qPCR ChIP-seq DB IF WB Affinity purified
H3K4me3 polyclonal antibody - Classic
(50 µl)
ChIP-qPCR DB ELISA WB Affinity purified
H3K4me3 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H3K4me3 polyclonal antibody - Premium
(50 µg/36 µl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K4me3T6p polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB IF IHC WB Affinity purified
H3K4un monoclonal antibody - Classic
(50 µg/50 µl)
ChIP-qPCR ELISA IF WB Protein A purified
H3K56ac polyclonal antibody - Classic
(50 μg/ 84 μl)
ChIP-qPCR ChIP-seq ELISA WB Affinity purified
H3K56me1 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB IF WB Affinity purified
H3K56me3 polyclonal antibody - Classic
(50 μg)
DB IF WB Affinity purified
H3K64me3 polyclonal antibody - Classic
(50 μg/ 84 μl)
ChIP-qPCR DB ELISA WB Affinity purified
H3K79me1 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H3K79me1 polyclonal antibody - Classic
(50 μg/31 μl)
ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
H3K79me2 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H3K79me2 polyclonal antibody - Classic
(50 μg/46 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K79me3 polyclonal antibody - Classic
(100 µl)
DB ELISA IF WB Whole antiserum
H3K79me3 polyclonal antibody - Classic
(50 µg/42 µl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K9/14ac polyclonal antibody - Classic
(50 μg/36 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K9/14ac polyclonal antibody - Premium
(50 μg/62 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
H3K9ac monoclonal antibody - Classic
(50 μg/50 μl)
ChIP-qPCR ELISA IF WB Protein A purified
H3K9ac polyclonal antibody - Classic
(50 µg/37 µl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K9ac polyclonal antibody - Classic
(50 μg/47 μl)
ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
H3K9acS10p polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA IF WB Whole antiserum
H3K9me1 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA IF WB Whole antiserum
H3K9me1 polyclonal antibody - Classic
(50 µg/43 µl)
ChIP-qPCR DB ELISA WB Affinity purified
H3K9me2 monoclonal antibody - Classic
(50 μg/50 μl)
ChIP-qPCR ELISA IF WB Protein A purified
H3K9me2 polyclonal antibody - Classic
(50 µg/44 µl)
ChIP-qPCR DB ELISA IF WB Affinity purified
H3K9me3 monoclonal antibody - Classic
(50 μg/25 μl)
ChIP-qPCR DB WB Protein A purified
H3K9me3 monoclonal antibody - Classic
(100 µl)
ChIP-qPCR DB WB Concentrated supernatant
H3K9me3 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA IF WB Whole antiserum
H3K9me3 polyclonal antibody - Classic
(50 µg/39 µl)
WB Protein G purified
H3K9me3 polyclonal antibody - Classic
(50 µg/33 µl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3K9me3 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR WB Whole antiserum
H3K9me3 polyclonal antibody - Premium
(50 μg/19 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
H3K9me3 recombinant antibody and negative control - Premium
(50 µg)
ChIP-qPCR ChIP-seq IF affinity purified
H3K9me3S10p polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA IF WB Whole antiserum
H3K9un monoclonal antibody - Classic
(50 μg/25 μl)
ChIP-qPCR IF WB Protein A purified
H3pan monoclonal antibody 1B1B2 - Classic
(50 μg/100 μl)
ChIP-qPCR IF WB Protein A purified
H3pan polyclonal antibody - Classic
(100 µl)
ChIP-qPCR ELISA WB Whole antiserum
H3pan polyclonal antibody - Classic
(50 μg/16 μl)
ChIP-qPCR ELISA WB Affinity purified
H3pan polyclonal antibody - Pioneer
(100 µl)
ELISA IF Whole antiserum
H3pan polyclonal antibody - Pioneer
(50 µg/53 µl)
ELISA WB Affinity purified
H3R17me2 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H3R17me2(asym) polyclonal antibody - Classic
(50 μg)
ChIP-qPCR IF WB Affinity purified
H3R17me2(asym)K18ac polyclonal antibody - Classic
(50 μg/53 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H3R2me1 polyclonal antibody - Classic
(50 μg)
WB Affinity purified
H3R2me2K4me2 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB Epigenetics DNA Methylation IF WB Affinity purified
H3R2me2sT3p polyclonal antibody - Classic
(50 μg)
DB IF WB Affinity purified
H3R8me2(asym) polyclonal antibody - Classic
(50 μg)
ChIP-qPCR IF IHC WB Affinity purified
H3R8me2(sym) polyclonal antibody - Classic
(50 μg)
ChIP-qPCR WB Affinity purified
H3S10p polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA IF IP WB Whole antiserum
H3S10p polyclonal antibody - Classic
(50 μg/48 μl)
ChIP-qPCR DB ELISA IF WB Affinity purified
H3S10pT11p polyclonal antibody - Classic
(50 μg)
IF IHC WB Affinity purified
H3T11p polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB ELISA IF WB Affinity purified
H3T3pK4ac polyclonal antibody - Classic
(50 μg)
DB IF IHC WB Affinity purified
H3T3pK4me1 polyclonal antibody - Classic
(50 μg)
DB IF IHC WB Affinity purified
H3T3pK4me2 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB IF IHC WB Affinity purified
H3T45p polyclonal antibody - Pioneer
(50 μg /34 μl)
DB ELISA Affinity purified
H3T6p polyclonal antibody - Classic
(50 μg)
DB IF IHC WB Affinity purified
H3T6pK9me1 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB IF IHC WB Affinity purified
H3T6pK9me2 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR IF IHC WB Affinity purified
H3T6pK9me3 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB IF IHC WB Affinity purified
H4K12ac monoclonal antibody - Classic
(50 µg)
ChIP-qPCR DB WB Protein A purified
H4K12ac polyclonal antibody - Classic
(50 μg)
DB IF IHC WB Affinity purified
H4K16ac monoclonal antibody - Classic
(50 µg)
ChIP-qPCR DB WB Protein A purified
H4K16ac polyclonal antibody - Classic
(50 μg)
DB Epigenetics DNA Methylation IF WB Affinity purified
H4K20me1 monoclonal antibody - Classic
(50 μg/23 μl)
ChIP-qPCR ChIP-seq DB IF WB Protein A purified
H4K20me1 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H4K20me1 polyclonal antibody - Classic
(50 μg/25 μl)
ChIP-qPCR DB ELISA IF WB Affinity purified
H4K20me1 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB WB Concentrated supernatant
H4K20me2 monoclonal antibody - Classic
(100 µg)
ChIP-qPCR IC IF WB Protein G purified
H4K20me2 monoclonal antibody - Classic
(50 µg)
ChIP-qPCR WB Protein A purified
H4K20me2 polyclonal antibody - Classic
(50 μg)
ChIP-qPCR IF WB Affinity purified
H4K20me3 monoclonal antibody - Classic
(100 µl)
ChIP-qPCR WB Concentrated supernatant
H4K20me3 polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H4K20me3 polyclonal antibody - Classic
(50 μg/47 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H4K20me3 polyclonal antibody - Premium
(50 μg/53 μl
(50 ChIP reactions))
ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
H4K5,8,12,16ac polyclonal antibody - Classic
(50 μg/66 μl)
ChIP-qPCR ChIP-seq DB IF Affinity purified
H4K5,8,12ac polyclonal antibody - Classic
(50 μg/66 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H4K5ac polyclonal antibody - Classic
(50 μg/28 μl)
ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
H4K8ac monoclonal antibody - Classic
(50 µg)
ChIP-qPCR DB WB Protein A purified
H4K8ac polyclonal antibody - Classic
(100 μg)
ChIP-qPCR DB IC IF IHC IP WB Affinity purified
H4K8ac polyclonal antibody - Classic
(100 µl)
ChIP-qPCR DB ELISA WB Whole antiserum
H4K8ac polyclonal antibody - Classic
(50 μg/41 μl)
ChIP-qPCR DB ELISA IF WB Affinity purified
H4pan polyclonal antibody - Classic
(50 μg/39 μl)
ChIP-qPCR ELISA WB Affinity purified
H4R3me1 polyclonal antibody - Classic
(50 μg)
DB IF WB Affinity purified
H4R3me2(sym) polyclonal antibody - Classic
(50 μg)
DB ELISA IF WB Affinity purified
H4S1p polyclonal antibody - Classic
(50 μg)
ChIP-qPCR DB IF IHC WB Affinity purified

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