Diagenode

H3R17me2(asym) polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15410289
50 μg
$370.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (asym-dimethyl Arg17), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.83 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Fig 1
Immunofluorescence 1:50 Fig 2
Western Blotting 1:500 Fig 3, 4
  • Validation Data

    Chromatin Immunoprecipitation

    Figure 1. Chromatin Immunoprecipitation
    Chromatin Immunoprecipitation of H3R17me2a antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of H3R17me2a antibody alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.

    Immunofluorescence

    Figure 2. Immunofluorescence
    Immunofluorescence of H3R17me2a antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3R17me2a is nuclear and chromosomal. Staining: Histone H3R17me2a is expressed in green, nuclei are counterstained with Dapi (blue).

    Western Blot

    Figure 3. Western Blot
    Western Blot of H3R17me2a antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Western Blot

    Figure 4. Western Blot
    Western Blot of H3R17me2a antibody. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

  •  実験手法
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet H3R17me2a C15410289 DATASHEET
    Datasheet description
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  •  出版物

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