Diagenode

H3R2me2K4me2 polyclonal antibody

Catalog Number
Format
Price
C15410294
50 μg
$480.00
  Bulk order



Polyclonal antibody raised in rabbit against Histone H3 (sym-dimethyl Arg2, dimethyl Lys4), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.91 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Figure 1
Immunofluorescence 1:100 Figure 2
Western Blotting 1:500 Figure 3, 4
Immunochemistry 1:50
Dot Blotting 1:1,000 Figure 5
  • Validation Data

    ChIP

    Figure 1. ChIP
    Chromatin Immunoprecipitation of H3R2me2sK4me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R2me2sK4me2 and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.

    Immunofluorescence

    Figure 2. Immunofluorescence
    Immunofluorescence of H3R2me2sK4me2 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3R2me2sK4me2 is nuclear and chromosomal. Staining: Histone H3R2me2sK4me2 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

    Western Blot

    Figure 3. Western Blot
    Western Blot of H3R2me2sK4me2 antibody. 30 μg of C. elegans embryo cell lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Western Blot

    Figure 4. Western Blot
    Western Blot of H3R2me2sK4me2 antibody. 30 μg of NIH-3T3 histone extracts. Primary antibody used at 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    Dot Blot

    Figure 5. Dot Blot
    Dot Blot of H3R2me2sK4me2 antibody. Lane 1: R2me2. Lane 2: R2me2/K4me2. Lane 3: K4me2. Load: 0.1, 1, 0.5, 5, and 10 picomoles of peptide. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  •  実験手法
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    DB
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet H3R2me2sK4me2 C15410294 DATASHEET
    Datasheet description
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  •  出版物

    How to properly cite this product in your work

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