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H2A.ZK7ac monoclonal antibody

カタログ番号
フォーマット
価格
C15210012
100 μg
$380.00
  Bulk order



Monoclonal antibody raised in rabbit against histone H2A.Z acetylated at Lys7 (H2A.ZK7ac), using a KLHconjugated synthetic peptide.

Lot001
Concentration1 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypeMonoclonal
PurityProtein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
HostRabbit
Storage ConditionsStore at -20°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 1 μg/ChIP Fig 1, 2
Western Blotting 1:2,000 Fig 3
Immunofluorescence 1:500 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.

  • Validation data

    H2A.ZK7ac Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against H2A.ZK7ac
    ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2A.ZK7ac (Cat. No. C15210012) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active p21 and FBXL18 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    H2A.ZK7ac Antibody ChIP-seq Grade

    H2A.ZK7ac Antibody for ChIP-seq

    H2A.ZK7ac Antibody for ChIP-seq assay

    H2A.ZK7ac Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H2A.ZK7ac
    ChIP was performed with 1 μg of the Diagenode antibody against H2A.ZK7ac (Cat. No. C15210012) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1.5 Mb region of the human X chromosome (figure 2A and B) and in two genomic regions surrounding the p21 and FBXL18 positive control genes (figure 2C and D).

    H2A.ZK7ac Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against H2A.ZK7ac
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H2A.ZK7ac (Cat. No. C15210012) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    H2A.ZK7ac Antibody validated in Immunofluorescence

    Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against H2A.ZK7ac
    HeLa cells treated with sodium butyrate were stained with the Diagenode antibody against H2A.ZK7ac (Cat. No. C15210012, red) diluted 1:500. Actin filaments were stained with fluorescein phalladoin (green).

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of H2A.ZK7 is associated with gene activation.

  •  実験手法
    ChIP-seq (ab)
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    ChIP-qPCR (ab)
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    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  •  資料
    H2A.ZK7ac monoclonal antibody DATASHEET
    Monoclonal antibody raised in rabbit against histone H2A.Z acetylated at Lys7 (H2A.ZK7ac), using ...
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  •  出版物

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