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H3R2me2sT3p polyclonal antibody

50 μg
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (p Thr3, Sym-dimethyl Arg2), using a KLH-conjugated synthetic peptide.

Concentration0.21 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Immunofluorescence 1:50-1:100 Figure 1
Western Blotting 1 μg/mL Figure 2
Dot Blotting 1:1,000 Figure 3
  • Validation Data


    Figure 1. H3R2me2sT3p Immunofluorescence results
    Immunofluorescence of H3R2me2sT3p. The H3R2me2sT3p antibody was tested at a 1:50 dilution in Hela cells with FITC (green). Nuclei were counterstained with DAPI (blue).

    Western blot

    Figure 2. H3R2me2sT3p Western blot results
    Western Blot of H3R2me2sT3p antibody. Western Blot analysis against untreated cell extracts. Lane 1: HeLa cell lysates. Lane 2: NIH/3T3 cell lysates. Lane 3: Cos 7 cell lysates. Load: 35 μg per lane. Primary antibody used at 1 μg/mL overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: 15 kDa. Other band(s): non-specific.

    Dot blot

    Figure 3. H3R2me2sT3p Dot blot results
    Dot Blot of H3R2me2sT3p antibody. Lane 1: Unmodified. Lane 2: R2me2s. Lane 3: T3p. Lane 4: R2me1. Lane 5: R2me2a. Lane 6: R2me2s. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1,000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40,000 for 30 min at RT.

  •  実験手法
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Dot blotting Read more
  •  資料
    Datasheet H3R2me2sT3p C15410303 DATASHEET
    Datasheet description
  •  出版物

    How to properly cite this product in your work

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