Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K23me2
ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3K23me2 (cat. No. C15210007) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 0.5, 1 and 2.5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for a region upstream of the promoters of the ACTB and GAPDH genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).