H3T45p polyclonal antibody

Catalog Number
50 μg /34 μl
  Bulk order

Polyclonal antibody raised in rabbit against the region of histone H3 containing the phosphorylated threonine 45 (H3T45p), using a KLH-conjugated synthetic peptide.

Concentration1.5 µg/µl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:5,000 - 1:10,000 Fig 1
Dot Blotting 1:20,000 Fig 2
  • Validation data


    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3T45p (cat. No. 15410321) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:350,000.

    Dot blot

    Figure 2. Cross reactivity tests using the Diagenode antibody directed against H3T45p
    To test the cross reactivity of the Diagenode antibody against H3T45p (cat. No. 15410321), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3T45. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

  •  実験手法
    Enzyme-linked immunosorbent assay. Read more
    Dot blotting Read more
  •  資料
    Datasheet H3T45p antibody DATASHEET
    Datasheet of H3T45p antibody
  •  Safety sheets
    H3T45p Antibody SDS GB en Download
    H3T45p Antibody SDS US en Download
    H3T45p Antibody SDS BE nl Download
    H3T45p Antibody SDS FR fr Download
    H3T45p Antibody SDS BE fr Download
    H3T45p Antibody SDS ES es Download
    H3T45p Antibody SDS DE de Download
    H3T45p Antibody SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3T45p polyclonal antibody (Diagenode Cat# C15410321 Lot# A2280P). Click here to copy to clipboard.

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