Diagenode

Epigenetics Chromatin Analysis

ChIP-qPCR

Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.



  1. Chromatin preparation: cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.
  2. Chromatin shearing: fragmentation of chromatin by sonication down to desired fragment size (100-500 bp)
  3. Chromatin IP: protein-DNA complexe capture using specific ChIP-grade antibodies against the histone or transcription factor of interest
  4. DNA purification: chromatin reverse cross-linking and elution followed by purification
  5. qPCR and analysis: using previously designed primers to amplify IP'd material at specific loci

Need guidance?

Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.

Made for ChIP-qPCR

Cat. No.ProductFormatPrice
C01010171 Auto iDeal ChIP-seq kit for Histones
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ...
100 rxns $1,995.00
C01010172 Auto iDeal ChIP-seq Kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
100 rxns $1,995.00
C01010140 Auto True MicroChIP kit
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This m...
16 rxns $495.00
C01010153 Auto Universal Plant ChIP-seq kit
The Auto Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, ma...
24 rxns $495.00
C01019027 ChIP Cross-link Gold
Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.However, formaldehyde is usually not effective to cross-li...
600 µl $125.00
C01010051 iDeal ChIP-seq kit for Histones
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns $995.00
C01010055 iDeal ChIP-seq kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
24 rxns $995.00
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit
C01010130 True MicroChIP Kit
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. T...
16 rxns $495.00
C01010152 Universal Plant ChIP-seq kit
The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, maize, ...
24 rxns $495.00
C02020011 Auto MethylCap kit
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCa...
48 rxns $495.00
C01010141 Auto True MicroChIP & MicroPlex Library Preparation™ Package
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation kit allows for performing ChIP-seq on as few as 10,000 cells. Thi...
16 ChIP rxns and 12 library prep rxns $1,550.00
B01020001 Bioruptor® Plus sonication device
The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (wate...
1 unit
C30080031 ChIPettor™ 96 Well Plate
Our ChIPettor™ 96 deep well plates are compatible with both the ChIPettor™ System for Histones and ChIPettor™ System for Transcription Fact...
5 pc $35.00
C01010160 ChIPettor™ System for Histones x10
The ChIPettor™ System for histones has everything you need for high-quality, fast, and easy chromatin immunoprecipitation: a full set of reagents for...
10 rxns $2,200.00
C01010162 ChIPettor™ System for Histones x24
The ChIPettor™ System for histones has everything you need for high-quality, fast, and easy chromatin immunoprecipitation: a full set of reagents for...
24 rxns $2,600.00
C01010161 ChIPettor™ System for Transcription Factors x10
The ChIPettor™ System for Transcription Factors has everything you need for high-quality, fast, and easy chromatin immunoprecipitation: a full set of...
10 rxns $2,200.00
C01010163 ChIPettor™ System for Transcription Factors x24
The ChIPettor™ System for Transcription Factors has everything you need for high-quality, fast, and easy chromatin immunoprecipitation: a full set of...
24 rxns $2,600.00
C30040010 ChIPettor™ Tips Protein A
Our ProA ChIPettor™ tips contain a packed-resin bed that keeps the ProA affinity resin contained within the tip end. The tips simplify the capture, pur...
12 tips/strip $115.00
C03030001 DNA co-precipitant
This IP grade product is optimized to be used as carrier for DNA purification after chromatin immunoprecipitation (ChIP) and after methylated DNA immunopreci...
100 µl $30.00
C03030002 DNA precipitant
This IP grade product is used for the precipitation of DNA after chromatin immunoprecipitation (ChIP) and after methylated DNA immunoprecipitation (Methyl DN...
1 ml $25.00
C03080030 DNA purifying slurry
The DNA purifying slurry can be used for easy and fast DNA purification and provides you with DNA suitable for qPCR analysis. If you need DNA of higher purit...
10 ml $145.00
C01010120 Elution Module
Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.
1 unit $125.00
C06060010 GenDNA RNAse (DNAse-free)
Our RNAse is DNAse-free. No boiling treatment is needed; it can be used directly from the vial. This product is particularly well suited for the use in methy...
10 µl $35.00
C01010063 HighCell# ChIP kit protein G
Perform ChIP with up to 10 million cells per IP. Recover more DNA and limit bias downstream applications.
48 rxns $1,235.00
C01010062 HighCell# ChIP kit protein A
Perform ChIP with up to 10 million cells per IP. Recover more DNA and limit bias downstream applications.
48 rxns $1,235.00
C01010100 Histone ChIP kit
Perform robust ChIP (Chromatin Immunoprecipitation) on 100.000 cells per IP. *Number of cells that can be lysed and sheared with this kit.
18 rxns $270.00
C01010053 iDeal ChIP-seq for Histones & Library Preparation Package (incl. Index Primer Set 1)
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns $1,885.00
C01010056 iDeal ChIP-seq for Transcription Factors & MicroPlex Library Preparation™ Package
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. Unlike comp...
10 ChIP and 12 library prep rxns $1,550.00
C01010173 Immunoprecipitation Buffers (iDeal ChIP-seq kit for Histones)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns $240.00
C01010174 Immunoprecipitation Buffers (iDeal ChIP-seq kit for TFs)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns $240.00
C01010072 LowCell# ChIP kit protein A
The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 100...
48 rxns $960.00
C01010073 LowCell# ChIP kit protein G
The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 100...
48 rxns $960.00
C17040004 Maize B73 inner stem ZmB1-UTR primer pair
This primer pair specifically amplifies a genomic region in the 5’-UTR of the B1 gene from Maize (Zea mays clone B73). The primers are thoroughly teste...
50 µl $30.00
C17040005 Maize B73 inner stem ZmCopia primer pair
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction ...
50 µl $30.00
C09010010 MethylTaq DNA polymerase
MethylTaq DNA polymerase is a high-performance Hot Start thermostable recombinant DNA polymerase. MethylTaq is an extremely robust modified Taq DNA polymeras...
250 units $215.00
C03040001 MicroChIP DiaPure columns
Diagenode's MicroChIP DiaPure columns are ideal in combination with the True MicroChIP kit allowing for the purification and elution of ChIP'd DNA in volumes...
50 rxns $95.00
C01010081 OneDay ChIP kit
Perform ChIP (Chromatin Immunoprecipitation) on large cell numbers. Check out our ChIP-Seq grade antibodies
180 rxns $720.00
C15410048 p53K372me1 polyclonal antibody - Classic
Alternative names: TP53, P53, TRP53, LSF1 Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), methylated at K372, using a KLH-conjug...
50 µg/25 µl $295.00
C01010150 Plant ChIP-seq kit
Diagenode's Plant ChIP-seq Kit is the only validated and complete solution for obtaining high quality ChIP-seq results from plant material. Specifically deve...
24 rxns $595.00
C17040011 Poplar xylem PtrCopia-orth primer pair
This primer pair specifically amplifies a genomic region from a sequence orthologous to the Maize Copia gene from poplar (Populus trichocarpa clone Nisqually...
50 µl $30.00
C17040010 Poplar xylem PtrMYBTF1 primer pair
This primer pair specifically amplifies a genomic region from a gene coding a MYB transcription factor in poplar (Populus trichocarpa clone Nisqually). The p...
50 µl $30.00
C17040008 Rice seedlings OsChr4-reg9 primer pair
This primer pair specifically amplifies a genomic region from chromosome 4 from Rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly t...
50 µl $30.00
C17040009 Rice seedlings OsMADS6 primer pair
This primer pair specifically amplifies a genomic region from the MADS6 gene from rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly...
50 µl $30.00
C17040006 Tomato leaves SlChr2-reg8 primer pair
This primer pair specifically amplifies a genomic region from chromosome 2 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl $30.00
C17040007 Tomato leaves SlChr4-NC1 primer pair
This primer pair specifically amplifies a genomic region from chromosome 4 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl $30.00
C01010090 Transcription ChIP Kit
form robust ChIP (Chromatin Immunoprecipitation) on large cell numbers. *Number of cells that can be lysed and sheared with this kit. >> Check out...
18 rxns $325.00
C01010131 True MicroChIP & MicroPlex Library Preparation™ Package
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microC...
16 ChIP rxns and 12 library prep rxns $1,550.00
C03030020 XL GenDNA Extraction Module
This module has been designed for the extraction of 60 µg of DNA from culture cells.
60 rxns $110.00

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