Diagenode

Picoruptor ® ソニケーター





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Best Workflow Practices for
ChIP-seq Analysis
Temperature-controlled
Easy to use
All-in-one solution
Processing of 6-16 samples
Sample size 5 µl – 2 ml
No soundproof box
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カタログ番号
フォーマット
B01060010
1 unit

Picoruptor は剪断の最新の技術革新であり、最大5μlから最大2mlまでの剪断マイクロボリュームのために最適化されたオールインワン剪断システムとしての新たなブレークスルーを表しています。 冷却システム(ウォータークーラーとシングルサイクルバルブ)により、高精度の温度制御が可能になり、より高品質のサンプルが得られます。

  • オールインワンシャーリングソリューション
  • 6-12個のサンプルの同時超音波処理
  • 小型で、軽く、使いやすい
  • 温度制御された

理想的な

  • 次世代シークエンシングのためのDNAシェアリング(5〜100μl)
  • クロマチン剪断(10μl〜2ml)
  • RNAシアリング
  • 組織及び細胞からのタンパク質抽出
  • FFPE DNA抽出

TESTIMONIAL

High throughput sequencing has dramatically reduced sequencing costs and the development of duel indexing strategies makes it possible to combine 384 samples in a single sequencing run. By developing library preparation methods to enrich for retroviral integration sites and retroviral transcripts we have been able to produce large numbers of low cost libraries to track the evolution of infection and to characterise patterns of expression from our virus of interest. The Bioruptor® Pico has been essential part of our protocols and provides straightforward and consistent shearing of our samples.

Dr Keith Durkin, Unit of Animal Genomics, GIGA-R, Université de Liège , 4000 Liège, Belgium
TESTIMONIAL

My group is mostly focused on epigenetic reprogramming and I have been using Diagenode products for the last 5 years. My experience with both antibodies, ChIP-Kits and the Bioruptor is nothing but positive. Diagenode products are unique for reproducibility, and this has always been a great plus for the success of my experiments.

Dr. Raffaele Teperino - Environmental Epigenetic Group - Institute of Experimental Genetics, Helmholtz Zentrum Muenchen GmbH, Germany
  • User manual
  • Recommended settings for DNA shearing with Bioruptor® Pico

    Follow our guidelines and find the good parameters for your expected DNA size: DNA shearing with the Bioruptor® Pico

  • お客様の声

    Neonatal screening cards provide a valuable bio-bank for retrospective studies. We have used the Bioruptor Pico and IP-Star Compact instrument along with the Auto MeDIP and IPure v2 kit to generate genome-wide DNA methylation profiles using only minute DNA inputs. Using the Diagenode instruments and products has generated reproducible results and saved us hands-on time. We are satisfied with the level of service and technical support and would recommend Diagenode to all others working in the field of epigenetics.

    Read the full abstract for Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots

    Dr. Nicklas Heine Staunstrup, Faculty of Health, Institute of Biomedicine, Aarhus University, Denmark

    I work with Diagenode’s Plant ChIP-seq kit and shear the DNA on the Bioruptor Pico for the last year and I have to say that these two products saved my PhD project! Some time ago, our well-established ChIP protocol suddenly stopped to work and after long time of figuring out the reason, we invested into Bioruptor Pico. I am very satisfied from the way it works, plus it’s super quiet! Combining the sonicator with the Plant ChIP-seq kit we finally got things working. I have also decided to try the Microplex Library Prep kit, which is amazing. I have been working with other kits and I find this one efficient and very easy to use. Recently, I have tested one of the epigenetics antibody (H3K4me3) and it works very well on the plant tissue, together with the ChIP-seq kit and Bioruptor.

    Thanks Diagenode for saving my PhD!

    Kamila Kwasniewska, Plant Developmental Genetics, Smurfit Institute, Trinity College, Dublin

    I have extensively used the antibodies against the histone modifications H3K4me3, H3k27me3, H3K9ac, H4k8ac and H3K18ac provided by Diagenode. The high level of specificity and selectivity of these antibodies in mouse brain samples, confirmed by using several negative and positive controls run in parallel with mouse brain tissue samples, ensured successful and reproducible results. I have been a Diagenode costumer for over one year now and I am extremely satisfied with the efficiency of the Bioruptor Pico for chromatin shearing as well as all of the ChIP materials (i.e., antibodies, blocking peptides, primer pairs for qPCR) provided by this company. Many thanks.

    Dr. Ermelinda Lomazzo, Institute of Physiological Chemistry, AG Prof. Beat Lutz. University Medical Center of the Johannes Gutenberg University Mainz, Germany

    We are particularly satisfied with the Bioruptor® Pico that we have recently purchased. Since we have started using this novel machine, all experiments for our epignetics research have been much more easy to perform. This machine is really a great thing for us. The whole department has now started using this novel device.

    Dr. Marco Pontoglio, Head of the Team "Expression Génique, Développement et Maladies" (EGDM) and Director of the “Development, Cancer and Reproduction”, Department Institut Cochin, Université Paris-Descartes

    Diagenode has provided a Bioruptor® Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially  for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.

    Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford

    The new Bioruptor® Pico machine has reduced the amount of time spent sonicating Chromatin by a massive amount. Some protocols require quite harsh fixing conditions which meant fragmenting DNA on the old machine was taking many rounds and several times. With the new Bioruptor® Pico machine these sonications were taking just one round of 10 cycles thereby reducing the fragmentation time substantially. Following sonication, I have used the new IDeal ChIP-seq kit. This is a nice straight forward kit that if followed with an appropriate chip validated antibody gave amazing chip-seq results that worked time and again with several different transcription factors. I would recommend both kits for good, consistant chromatin work.

    Dr. Karen Dawson, RNA Biology Group, Cancer Research UK Manchester Institute at the University of Manchester

    The Bioruptor® NGS* is a robust user-friendly sonication system that is key for both reproducible chromatin shearing and DNA shearing. The Bioruptor® NGS consistently delivered optimal DNA shearing that was critical in generating homogeneous sequencing libraries for our experiments. We also observed excellent chromatin shearing result that were crucial for our epigenetic mapping projects.

    Dr. Sascha Tierling, Department of Genetics and Epigenetics, Prof . Dr. Jörn Walter's lab, Universität des Saarlandes, Germany.
  • プロトコル集
    Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico
    Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c...
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    Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico
    This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro...
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    Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl
    HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleu...
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    Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml
    Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin Shearing...
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    Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder
    The first critical step of a successful ChIP experiment is the preparation of sheared chromatin w...
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    Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®
    Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene...
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    DNA Shearing for Bioruptor® Pico - Standard 100 μl Volume
    Standard protocols for programmable DNA size distributions, excellent reproducibility, and high d...
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    DNA Shearing for Bioruptor® Pico - 50 μl Volume
    For DNA shearing in 50 μl we highly recommend to use the 0.1 ml tube holder & tube adaptor...
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    DNA shearing for Next-Generation Sequencing with the Bioruptor® pico, 0.2 ml Bioruptor® microtubes and the corresponding holder
    Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, amo...
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    DNA Shearing with Bioruptor® Pico - Small Volume (10 µl)
    For DNA shearing we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. No. ...
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    FFPE DNA extraction protocol using the Bioruptor®
    The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, provid...
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    iST Sample Preparation with the Bioruptor®
    The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e...
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    PCR amplicons shearing using the Bioruptor Pico
    With the Bioruptor® Pico and the former model Bioruptor® NGS it is possible to shear PCR ...
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    Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
    Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...
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    Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico
    ChIP-seq is a common tool used to study protein-DNA interactions in cells and tissues on a genome...
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    RNA Shearing for Bioruptor® NGS and Pico
    The Diagenode ACT (Adaptative Cavitation Transfer technology) process is highly reproducible, how...
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    Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation
    Histology-defined proteomics investigations are a fundamental tool in modern molecular pathology....
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  • アプリケーション
    DNA/RNA断片
    近年の次世代シーケンシング(NGS)の進歩はゲノミクスや生物学に革命をもたらしました。 この成長により、最適なサンプル調製、ゲノムライブラリー構築には欠かせない高度技術の要求、必要性がさらに高まっています。 最適なライブラリー調製に最も重要な要因の一つは、シーケンシングされるDNAの品質です。 まずは、DNAを効果的かつ一貫してシーケンシングプラットフォームに応じた適切な断片サイズに切断し、具体的で信頼できるNGS結果を実現する必要があります。 Picoruptor and Megar... Read more
    クロマチン断片
    ChIPの成功に最も重要なステップは細胞の固定と溶解、そしてクロマチンの断片化の両方です。 研究者の中には、これらのステップの両方の重要な性質を見過すことがあります。断片化工程の不一致を解消するDiagenodeのPicoruptor®は、最先端の超音波ACT(Adaptive Cavitation Technology)を駆使し、効率的にクロマチンを断片します。ACTは、穏やかかつ非常に有効な断片力を用いて、ChIP実験に必須の高いIP効率および感度を持つ最高のクロマチン品質... Read more
    ホルマリン固定 DNA抽出
    Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraf... Read more
    次世代シーケンシング
    DNAせん断、ライブラリー作成、自動化:NGSのワンストップショップ 1. せん断装置を選択してください:150 bp〜75 kbの範囲でDNAをせん断します。 5μlまでせん断:150 bp〜2 kbNGS DNAライブラリー調製およびFFPE核酸抽出に最適で、 2 kb〜75 kbの範囲をせんできます。メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイス... Read more
    ChIP-seq
    検出方法(ChIP-seq)としてハイスループットシーケンシングと結合したクロマチン免疫沈降(ChIP)は、今やエピゲノム研究、すなわちゲノム規模でのタンパク質-DNA相互作用の研究において主要な方法の1つされています。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。 ChIP-seqワークフロー クロマチン調製 : DNAへのヒストンまたは転... Read more
    タンパク質抽出
    Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such... Read more
    メチル化DNA結合タンパク質
    The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containin... Read more
    エピジェネティクス・クロマチン解析
    エピジェネティクス研究は、異なる転写パターン、遺伝子発現およびサイレンシングを引き起こすクロマチンの変化に対処します。クロマチンの主成分はDNAおよびヒストン蛋白質です。 各ヒストンコア蛋白質(H2A、H2B、H3およびH4)の2つのコピーを8量体に組み込み、DNAで包んでヌクレオソームコアを形成させます。 ヌクレオソームは、転写機械のDNAへの接近可能性およびクロマチン再構成因子を制御します。 クロマチン免疫沈降(ChIP)は、関心対象の特定の蛋白質に対... Read more
  • 資料
    Bioruptor® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
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    Bioruptor® DNA shearing for Agilent SureSelect library preparation: a perfect combination APPLICATION NOTE
    The Cologne Center for Genomics (CCG), headed by Prof. Dr. Peter Nürnberg, shows that t...
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    Which tubes for which Bioruptor®? QUICK GUIDE
    Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu...
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    Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing CASE STUDY
    Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center,...
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    True MicroChIP and MicroPlex kits APPLICATION NOTE
    From minuscule amounts to magnificent results: reliable ChIP-seq data from 10,000 cells with the ...
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    ChIP-seq from human tumor tissue APPLICATION NOTE
    Application of the Bioruptor® Pico for shearing chromatin fragments for ChIP-seq from human t...
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    ChIP kit results with True MicroChIP kit POSTER
    Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become the g...
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    Bioruptor® NGS System – Designed Exclusively for Fast & Accurate DNA Shearing POSTER
    The Bioruptor®NGS is the DNA shearing device of choice for sequencing applications, providing...
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    Application of the Bioruptor® Pico for best shearing of yeast genomic DNA for NGS APPLICATION NOTE
    DNA shearing is important step for next-generation sequencing library preparation. Important is r...
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    DNA shearing for Next-Generation Sequencing with the Bioruptor® pico, 0.2 ml Bioruptor® microtubes and the corresponding holder PROTOCOL
    Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, amo...
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    Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder PROTOCOL
    The first critical step of a successful ChIP experiment is the preparation of sheared chromatin w...
    Download
    Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing APPLICATION NOTE
    Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation o...
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    Efficient and streamlined solution for high-quality NGS library preparation using the Bioruptor® Pico and JetSeqTM Flex DNA Library Preparation Kit APPLICATION NOTE
    Next Generation Sequencing workflows include a number of critical steps which can have a signific...
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    Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico APPLICATION NOTE
    The chromatin shearing is very important step for all kind of ChIP analysis followed by next-gene...
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    Ultrasensitive proteomics for microdissected fresh frozen tissue samples APPLICATION NOTE
    This protocol, specifically designed for ultrasensitive proteomics, enables high throughput quant...
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    Bioruptor® Pico Sonication System Manual MANUAL
    The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as...
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  • 出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Picoruptor ® ソニケーター (Diagenode Cat# B01060010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Modulation of p53 and met expression by Krüppel-like factor 8 regulates zebrafish cerebellar development.
    Tsai MY, Lu YF, Liu YH, Lien HW, Huang CJ, Wu JL, Hwang SL
    Krüppel-like factor 8 (Klf8) is a zinc-finger transcription factor implicated in cell proliferation, and cancer cell survival and invasion; however, little is known about its role in normal embryonic development. Here, we show that Klf8 is required for normal cerebellar development in zebrafish embryos. Morphol...

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