日本国内のみ販売中
Picoruptorは、微小容量の5μlから最大2mlの大容量のせん断に最適化されたオールインワンせん断システムで、断片化における最新の革新技術です。内蔵の冷却システム（Picoruptor Cooler）により、高精度の温度制御が保証され、より高品質のサンプルが得られます。
適した用途
Picoruptorについてのショートビデオでは、クロマチンシアリング、NGS用のDNAシアリング、FFPEサンプルからの比類のないDNA抽出、RNAシアリング、タンパク質抽出など、あらゆるアプリケーションで完璧なシアリングを実現する方法をご覧いただけます。
TESTIMONIALWe at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.
Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany
TESTIMONIALHigh throughput sequencing has dramatically reduced sequencing costs and the development of duel indexing strategies makes it possible to combine 384 samples in a single sequencing run. By developing library preparation methods to enrich for retroviral integration sites and retroviral transcripts we have been able to produce large numbers of low cost libraries to track the evolution of infection and to characterise patterns of expression from our virus of interest. The Bioruptor® Pico has been essential part of our protocols and provides straightforward and consistent shearing of our samples.
Dr Keith Durkin, Unit of Animal Genomics, GIGA-R, Université de Liège , 4000 Liège, Belgium
TESTIMONIALMy group is mostly focused on epigenetic reprogramming and I have been using Diagenode products for the last 5 years. My experience with both antibodies, ChIP-Kits and the Bioruptor is nothing but positive. Diagenode products are unique for reproducibility, and this has always been a great plus for the success of my experiments.
Dr. Raffaele Teperino - Environmental Epigenetic Group - Institute of Experimental Genetics, Helmholtz Zentrum Muenchen GmbH, Germany
Follow our guidelines and find the good parameters for your expected DNA size: DNA shearing with the Bioruptor® Pico
Neonatal screening cards provide a valuable bio-bank for retrospective studies. We have used the Bioruptor Pico and IP-Star Compact instrument along with the Auto MeDIP and IPure v2 kit to generate genome-wide DNA methylation profiles using only minute DNA inputs. Using the Diagenode instruments and products has generated reproducible results and saved us hands-on time. We are satisfied with the level of service and technical support and would recommend Diagenode to all others working in the field of epigenetics.
Dr. Nicklas Heine Staunstrup, Faculty of Health, Institute of Biomedicine, Aarhus University, Denmark
I work with Diagenode’s Plant ChIP-seq kit and shear the DNA on the Bioruptor Pico for the last year and I have to say that these two products saved my PhD project! Some time ago, our well-established ChIP protocol suddenly stopped to work and after long time of figuring out the reason, we invested into Bioruptor Pico. I am very satisfied from the way it works, plus it’s super quiet! Combining the sonicator with the Plant ChIP-seq kit we finally got things working. I have also decided to try the Microplex Library Prep kit, which is amazing. I have been working with other kits and I find this one efficient and very easy to use. Recently, I have tested one of the epigenetics antibody (H3K4me3) and it works very well on the plant tissue, together with the ChIP-seq kit and Bioruptor.
Thanks Diagenode for saving my PhD!
Kamila Kwasniewska, Plant Developmental Genetics, Smurfit Institute, Trinity College, Dublin
I have extensively used the antibodies against the histone modifications H3K4me3, H3k27me3, H3K9ac, H4k8ac and H3K18ac provided by Diagenode. The high level of specificity and selectivity of these antibodies in mouse brain samples, confirmed by using several negative and positive controls run in parallel with mouse brain tissue samples, ensured successful and reproducible results. I have been a Diagenode costumer for over one year now and I am extremely satisfied with the efficiency of the Bioruptor Pico for chromatin shearing as well as all of the ChIP materials (i.e., antibodies, blocking peptides, primer pairs for qPCR) provided by this company. Many thanks.
Dr. Ermelinda Lomazzo, Institute of Physiological Chemistry, AG Prof. Beat Lutz. University Medical Center of the Johannes Gutenberg University Mainz, Germany
We are particularly satisfied with the Bioruptor® Pico that we have recently purchased. Since we have started using this novel machine, all experiments for our epignetics research have been much more easy to perform. This machine is really a great thing for us. The whole department has now started using this novel device.
Dr. Marco Pontoglio, Head of the Team "Expression Génique, Développement et Maladies" (EGDM) and Director of the “Development, Cancer and Reproduction”, Department Institut Cochin, Université Paris-Descartes
Diagenode has provided a Bioruptor® Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.
Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford
The new Bioruptor® Pico machine has reduced the amount of time spent sonicating Chromatin by a massive amount. Some protocols require quite harsh fixing conditions which meant fragmenting DNA on the old machine was taking many rounds and several times. With the new Bioruptor® Pico machine these sonications were taking just one round of 10 cycles thereby reducing the fragmentation time substantially. Following sonication, I have used the new IDeal ChIP-seq kit. This is a nice straight forward kit that if followed with an appropriate chip validated antibody gave amazing chip-seq results that worked time and again with several different transcription factors. I would recommend both kits for good, consistant chromatin work.
Dr. Karen Dawson, RNA Biology Group, Cancer Research UK Manchester Institute at the University of Manchester
The Bioruptor® NGS* is a robust user-friendly sonication system that is key for both reproducible chromatin shearing and DNA shearing. The Bioruptor® NGS consistently delivered optimal DNA shearing that was critical in generating homogeneous sequencing libraries for our experiments. We also observed excellent chromatin shearing result that were crucial for our epigenetic mapping projects.
Dr. Sascha Tierling, Department of Genetics and Epigenetics, Prof . Dr. Jörn Walter's lab, Universität des Saarlandes, Germany.
| Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c... | Download |
| Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro... | Download |
| Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleu... | Download |
| Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShea... | Download |
| Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder The first critical step of a successful ChIP experiment is the preparation of sheared chromatin w... | Download |
| Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor® Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene... | Download |
| DNA Shearing for Bioruptor® Pico (2013-2019) - Standard 100 μl Volume Standard protocols for programmable DNA size distributions, excellent reproducibility, and high d... | Download |
| DNA Shearing for Bioruptor® Pico (2013-2019) - 50 μl Volume For DNA shearing in 50 μl we highly recommend to use the 0.1 ml tube holder & tube adaptor... | Download |
| DNA shearing for Next-Generation Sequencing with the Bioruptor® Pico (2013-2019), 0.2 ml Bioruptor® microtubes and the corresponding holder Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, amo... | Download |
| DNA Shearing with Bioruptor® Pico (2013-2019) - Small Volume (10 µl) For DNA shearing we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. No. ... | Download |
| FFPE DNA extraction protocol using the Bioruptor® The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, provid... | Download |
| iST Sample Preparation with the Bioruptor® The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e... | Download |
| PCR amplicons shearing using the Bioruptor Pico With the Bioruptor® Pico and the former model Bioruptor® NGS it is possible to shear PCR ... | Download |
| Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico Protein extraction from tissues and cultured cells is the first step for many biochemical and ana... | Download |
| Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico ChIP-seq is a common tool used to study protein-DNA interactions in cells and tissues on a genome... | Download |
| RNA Shearing for Bioruptor® NGS and Pico The Diagenode ACT (Adaptative Cavitation Transfer technology) process is highly reproducible, how... | Download |
| Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation Histology-defined proteomics investigations are a fundamental tool in modern molecular pathology.... | Download |
It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.
| Chromatin Shearing Kit Low SDS (for Histones) | Chromatin Shearing Kit Low SDS (for TF) | Chromatin Shearing Kit High SDS | Chromatin Shearing Kit (for Plant) | |
|
SDS concentration |
< 0.1% |
0.2% |
1% |
0.5% |
|
Nuclei isolation |
Yes |
Yes |
No |
Yes |
|
Allows for shearing of... cells/tissue |
100 million cells |
100 million cells |
100 million cells |
up to 25 g of tissue |
|
Corresponding to shearing buffers from |
| Bioruptor ® Sonicator Brochure BROCHURE Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp... | Download |
| Bioruptor® DNA shearing for Agilent SureSelect library preparation: a perfect combination APPLICATION NOTE The Cologne Center for Genomics (CCG), headed by Prof. Dr. Peter Nürnberg, shows that t... | Download |
| Which tubes for which Bioruptor®? QUICK GUIDE Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu... | Download |
| Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing CASE STUDY Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center,... | Download |
| True MicroChIP and MicroPlex kits APPLICATION NOTE From minuscule amounts to magnificent results: reliable ChIP-seq data from 10,000 cells with the ... | Download |
| ChIP-seq from human tumor tissue APPLICATION NOTE Application of the Bioruptor® Pico for shearing chromatin fragments for ChIP-seq from human t... | Download |
| ChIP kit results with True MicroChIP kit POSTER Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become the g... | Download |
| Bioruptor® NGS System – Designed Exclusively for Fast & Accurate DNA Shearing POSTER The Bioruptor®NGS is the DNA shearing device of choice for sequencing applications, providing... | Download |
| Application of the Bioruptor® Pico for best shearing of yeast genomic DNA for NGS APPLICATION NOTE DNA shearing is important step for next-generation sequencing library preparation. Important is r... | Download |
| DNA shearing for Next-Generation Sequencing with the Bioruptor® pico, 0.2 ml Bioruptor® microtubes and the corresponding holder PROTOCOL Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, amo... | Download |
| Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder PROTOCOL The first critical step of a successful ChIP experiment is the preparation of sheared chromatin w... | Download |
| Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing APPLICATION NOTE Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation o... | Download |
| Efficient and streamlined solution for high-quality NGS library preparation using the Bioruptor® Pico and JetSeqTM Flex DNA Library Preparation Kit APPLICATION NOTE Next Generation Sequencing workflows include a number of critical steps which can have a signific... | Download |
| Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico APPLICATION NOTE The chromatin shearing is very important step for all kind of ChIP analysis followed by next-gene... | Download |
| Ultrasensitive proteomics for microdissected fresh frozen tissue samples APPLICATION NOTE This protocol, specifically designed for ultrasensitive proteomics, enables high throughput quant... | Download |
| Bioruptor® Pico Sonication System Manual MANUAL The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as... | Download |
| Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology APPLICATION NOTE Dr. Kelvin Luk’s lab at the University of Pennsylvania focuses on understanding Parkinson&r... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Picoruptor Sonicator (Diagenode Cat# B01060010). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Distinct silencer states generate epigenetic states of heterochromatin. |
RAD51 protects human cells from transcription-replication conflicts. |
Lasp1 regulates adherens junction dynamics and fibroblast transformationin destructive arthritis |
Corticosteroid receptors adopt distinct cyclical transcriptional signatures |
BCG Vaccination Protects against Experimental Viral Infection in Humans through the Induction of Cytokines Associated with Trained Immunity. |
Modulation of p53 and met expression by Krüppel-like factor 8 regulates zebrafish cerebellar development. |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => 'Picoruptor, sonication, Next-Generation-Sequencing,DNA shearing,Protein extraction', 'meta_description' => 'オールインワンシャーリングシステムは次世代シークエンシング、クロマチンシアリング、RNAシアリング、組織および細胞からのタンパク質抽出、FFPE DNA抽出のためのDNAシャーリングに最適です。', 'meta_title' => 'Picoruptor 超音波装置は、次世代シーケンシング用RNA、クロマチンおよびDNAを切断| Diagenode', 'product' => array( 'Product' => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Picoruptor Sonicator', 'description' => '<p>日本国内のみ販売中</p> <p></p> <p>Picoruptorは、微小容量の5μlから最大2mlの大容量のせん断に最適化されたオールインワンせん断システムで、断片化における最新の革新技術です。内蔵の冷却システム（Picoruptor Cooler）により、高精度の温度制御が保証され、より高品質のサンプルが得られます。</p> <ul> <li>オールインワン断片化ソリューション：完全検証済のチューブとキットが利用可能</li> <li>6-16サンプルを同時超音波処理</li> <li>小さく、軽く、使いやすい</li> <li>温度制御</li> </ul> <p>適した用途</p> <ul> <li>次世代シーケンシングのためのDNAシェアリング（5〜100 µl）</li> <li>クロマチン断片化（10 µl-2 ml）</li> <li>RNA断片化</li> <li>組織および細胞からのタンパク質抽出</li> <li>FFPE DNA抽出</li> <li>タンパク質凝集研究</li> </ul> <p></p> <script>// <![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> <center><iframe width="560" height="315" src="https://www.youtube.com/embed/ckLc4owudIM" frameborder="0" allowfullscreen="allowfullscreen"></iframe></center><center> <p><small>Picoruptorについてのショートビデオでは、クロマチンシアリング、NGS用のDNAシアリング、FFPEサンプルからの比類のないDNA抽出、RNAシアリング、タンパク質抽出など、あらゆるアプリケーションで完璧なシアリングを実現する方法をご覧いただけます。</small></p> </center><center><a href="https://www.diagenode.com/en/pages/osha"><img src="https://www.diagenode.com/img/banners/banner-osha-580.jpg" width="635" height="243" /></a></center> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => 'ユーザーガイド', 'info1' => '<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/Bioruptor_pico_cooler_manual.pdf">Download</a></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'PicoruptorによるDNA断片化の推奨設定', 'info2' => '<p>Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor® Pico</a></p> <p></p> <p> <script>// <![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> </p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => 'ご利用可能なChromatin Shearing kit', 'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01060010', 'old_catalog_number' => '', 'sf_code' => 'B01060010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '22700', 'price_USD' => '26250', 'price_GBP' => '20150', 'price_JPY' => '3291500', 'price_CNY' => 'Discontinued', 'price_AUD' => '65625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-pico-sonication-device', 'meta_title' => 'Picoruptor 超音波装置は、次世代シーケンシング用RNA、クロマチンおよびDNAを切断| Diagenode', 'meta_keywords' => 'Picoruptor, sonication, Next-Generation-Sequencing,DNA shearing,Protein extraction', 'meta_description' => 'オールインワンシャーリングシステムは次世代シークエンシング、クロマチンシアリング、RNAシアリング、組織および細胞からのタンパク質抽出、FFPE DNA抽出のためのDNAシャーリングに最適です。', 'modified' => '2021-07-14 13:47:33', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ) ), 'Feature' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Image' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Testimonial' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ) ), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = 'Picoruptor, sonication, Next-Generation-Sequencing,DNA shearing,Protein extraction' $meta_description = 'オールインワンシャーリングシステムは次世代シークエンシング、クロマチンシアリング、RNAシアリング、組織および細胞からのタンパク質抽出、FFPE DNA抽出のためのDNAシャーリングに最適です。' $meta_title = 'Picoruptor 超音波装置は、次世代シーケンシング用RNA、クロマチンおよびDNAを切断| Diagenode' $product = array( 'Product' => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Picoruptor Sonicator', 'description' => '<p>日本国内のみ販売中</p> <p></p> <p>Picoruptorは、微小容量の5μlから最大2mlの大容量のせん断に最適化されたオールインワンせん断システムで、断片化における最新の革新技術です。内蔵の冷却システム（Picoruptor Cooler）により、高精度の温度制御が保証され、より高品質のサンプルが得られます。</p> <ul> <li>オールインワン断片化ソリューション：完全検証済のチューブとキットが利用可能</li> <li>6-16サンプルを同時超音波処理</li> <li>小さく、軽く、使いやすい</li> <li>温度制御</li> </ul> <p>適した用途</p> <ul> <li>次世代シーケンシングのためのDNAシェアリング（5〜100 µl）</li> <li>クロマチン断片化（10 µl-2 ml）</li> <li>RNA断片化</li> <li>組織および細胞からのタンパク質抽出</li> <li>FFPE DNA抽出</li> <li>タンパク質凝集研究</li> </ul> <p></p> <script>// <![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> <center><iframe width="560" height="315" src="https://www.youtube.com/embed/ckLc4owudIM" frameborder="0" allowfullscreen="allowfullscreen"></iframe></center><center> <p><small>Picoruptorについてのショートビデオでは、クロマチンシアリング、NGS用のDNAシアリング、FFPEサンプルからの比類のないDNA抽出、RNAシアリング、タンパク質抽出など、あらゆるアプリケーションで完璧なシアリングを実現する方法をご覧いただけます。</small></p> </center><center><a href="https://www.diagenode.com/en/pages/osha"><img src="https://www.diagenode.com/img/banners/banner-osha-580.jpg" width="635" height="243" /></a></center> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => 'ユーザーガイド', 'info1' => '<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/Bioruptor_pico_cooler_manual.pdf">Download</a></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'PicoruptorによるDNA断片化の推奨設定', 'info2' => '<p>Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor® Pico</a></p> <p></p> <p> <script>// <![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> </p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => 'ご利用可能なChromatin Shearing kit', 'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01060010', 'old_catalog_number' => '', 'sf_code' => 'B01060010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '22700', 'price_USD' => '26250', 'price_GBP' => '20150', 'price_JPY' => '3291500', 'price_CNY' => 'Discontinued', 'price_AUD' => '65625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-pico-sonication-device', 'meta_title' => 'Picoruptor 超音波装置は、次世代シーケンシング用RNA、クロマチンおよびDNAを切断| Diagenode', 'meta_keywords' => 'Picoruptor, sonication, Next-Generation-Sequencing,DNA shearing,Protein extraction', 'meta_description' => 'オールインワンシャーリングシステムは次世代シークエンシング、クロマチンシアリング、RNAシアリング、組織および細胞からのタンパク質抽出、FFPE DNA抽出のためのDNAシャーリングに最適です。', 'modified' => '2021-07-14 13:47:33', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '1796', 'antibody_id' => null, 'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '1 pack', 'catalog_number' => 'B01200016', 'old_catalog_number' => 'O-ring-15', 'sf_code' => 'B01200016-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '300', 'price_USD' => '310', 'price_GBP' => '260', 'price_JPY' => '43900', 'price_CNY' => '', 'price_AUD' => '775', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack', 'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'meta_keywords' => '', 'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'modified' => '2022-01-25 04:00:28', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2624', 'antibody_id' => null, 'name' => '0.65 ml Picoruptor Microtubes', 'description' => '<p><span>The 0.65 ml Picoruptor Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Picoruptor (B0106000) or Bioruptor® NGS (UCD-600) and are strongly recommended for DNA and chromatin shearing applications using these instruments. Recommended sample volume: 100 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '500 tubes', 'catalog_number' => 'C30010011', 'old_catalog_number' => 'WA-005-0500', 'sf_code' => 'C30010011-1862', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '145', 'price_USD' => '235', 'price_GBP' => '130', 'price_JPY' => '21200', 'price_CNY' => '', 'price_AUD' => '590', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-65-ml-bioruptor-microtubes-500-tubes', 'meta_title' => '0.65 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.65 ml Bioruptor® Microtubes', 'modified' => '2022-07-19 05:03:16', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2629', 'antibody_id' => null, 'name' => '0.1 ml Bioruptor<sup>®</sup> Pico Microtubes', 'description' => '<p><span>The 0.1 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico (B01060001) and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: </span><span>5 μl - 50 μl.</span></p> <div class="page" title="Page 1"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '120 tubes', 'catalog_number' => 'C30010015', 'old_catalog_number' => '', 'sf_code' => 'C30010015-5702', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '150', 'price_USD' => '215', 'price_GBP' => '130', 'price_JPY' => '22500', 'price_CNY' => '', 'price_AUD' => '540', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '0-1-ml-bioruptor-microtubes-120-tubes', 'meta_title' => '0.1 ml Bioruptor® Microtubes', 'meta_keywords' => '', 'meta_description' => '0.1 ml Bioruptor® Microtubes', 'modified' => '2021-12-20 12:31:40', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2630', 'antibody_id' => null, 'name' => '1.5 ml Picoruptor Microtubes with Caps', 'description' => '<p><span>The 1.5 ml Picoruptor Microtubes with Caps for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '300 tubes', 'catalog_number' => 'C30010016', 'old_catalog_number' => '', 'sf_code' => 'C30010016-5700', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '100', 'price_USD' => '145', 'price_GBP' => '95', 'price_JPY' => '15600', 'price_CNY' => '', 'price_AUD' => '365', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '1-5-ml-bioruptor-microtubes-with-caps-300-tubes', 'meta_title' => '1.5 ml Bioruptor® Microtubes with Caps', 'meta_keywords' => '', 'meta_description' => '1.5 ml Bioruptor® Microtubes with Caps', 'modified' => '2022-07-19 05:04:02', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1875', 'antibody_id' => null, 'name' => '15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads', 'description' => '<p><span>The 15 ml Bioruptor® Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Bioruptor® Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 rxns', 'catalog_number' => 'C01020031', 'old_catalog_number' => '', 'sf_code' => 'C01020031-6000', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '150', 'price_USD' => '215', 'price_GBP' => '140', 'price_JPY' => '22500', 'price_CNY' => '', 'price_AUD' => '540', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns', 'meta_title' => '15 ml Bioruptor® Tubes & sonication beads', 'meta_keywords' => '', 'meta_description' => '15 ml Bioruptor® Tubes & sonication beads', 'modified' => '2021-12-20 12:27:43', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1869', 'antibody_id' => null, 'name' => 'Chromatin EasyShear Kit - High SDS', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/Chromatin_Shearing_kit_HighSDS_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p style="text-align: justify;">Previous name: Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)</p> <p style="text-align: justify;">A high quality chromatin preparation is very complex and requires a lot of optimization. Chromatin EasyShear Kit – High SDS is an optimized solution for efficient chromatin preparation prior to ChIP. The protocol, buffers composition, SDS concentration (1%) is optimized for the preparation of chromatin prior to ChIP on low amount of starting material<b> </b>and it is compatible with Diagenode's <a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP-seq kit</a> and <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation Kit for Histones</a>. The kit has been validated with the Bioruptor ultrasonicator for efficient chromatin shearing, leading to chromatin fragments<span> </span><strong>suitable for ChIP</strong><span> </span>with the preserved<span> </span><strong>epitopes</strong>.</p> <p style="text-align: justify;">Check all <a href="https://www.diagenode.com/en/categories/chromatin-shearing">Chromatin EasyShear Kits</a>.</p> <p style="text-align: justify;">Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – <a href="https://www.diagenode.com/en/pages/chromatin-prep-easyshear-kit-guide">Read more</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 million cells', 'catalog_number' => 'C01020012', 'old_catalog_number' => 'AA-003-0100', 'sf_code' => 'C01020012-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '290', 'price_USD' => '325', 'price_GBP' => '265', 'price_JPY' => '50800', 'price_CNY' => '', 'price_AUD' => '815', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'chromatin-shearing-optimization-kit-high-sds-100-million-cells', 'meta_title' => 'Chromatin shearing optimization kit - High SDS', 'meta_keywords' => '', 'meta_description' => 'Chromatin shearing optimization kit - High SDS', 'modified' => '2023-04-20 16:33:08', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '1836', 'antibody_id' => null, 'name' => 'iDeal ChIP-seq kit for Histones', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/ideal-chipseq-for-histones-complete-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>Don’t risk wasting your precious sequencing samples. Diagenode’s validated <strong>iDeal ChIP-seq kit for Histones</strong> has everything you need for a successful start-to-finish <strong>ChIP of histones prior to Next-Generation Sequencing</strong>. The complete kit contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation and DNA purification. In addition, unlike competing solutions, the kit contains positive and negative control antibodies (H3K4me3 and IgG, respectively) as well as positive and negative control PCR primers pairs (GAPDH TSS and Myoglobin exon 2, respectively) for your convenience and a guarantee of optimal results. The kit has been validated on multiple histone marks.</p> <p> The iDeal ChIP-seq kit for Histones<strong> </strong>is perfect for <strong>cells</strong> (<strong>100,000 cells</strong> to <strong>1,000,000 cells</strong> per IP) and has been validated for <strong>tissues</strong> (<strong>1.5 mg</strong> to <strong>5 mg</strong> of tissue per IP).</p> <p> The iDeal ChIP-seq kit is the only kit on the market validated for the major sequencing systems. Our expertise in ChIP-seq tools allows reproducible and efficient results every time.</p> <p></p> <p> <strong></strong></p> <p></p>', 'label1' => 'Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li>Highly <strong>optimized</strong> protocol for ChIP-seq from cells and tissues</li> <li><strong>Validated</strong> for ChIP-seq with multiple histones marks</li> <li>Most <strong>complete</strong> kit available (covers all steps, including the control antibodies and primers)</li> <li>Optimized chromatin preparation in combination with the Bioruptor ensuring the best <strong>epitope integrity</strong></li> <li>Magnetic beads make ChIP easy, fast and more <strong>reproducible</strong></li> <li>Combination with Diagenode ChIP-seq antibodies provides high yields with excellent <strong>specificity</strong> and <strong>sensitivity</strong></li> <li>Purified DNA suitable for any downstream application</li> <li>Easy-to-follow protocol</li> </ul> <p>Note: to obtain optimal results, this kit should be used in combination with the DiaMag1.5 - magnetic rack.</p> <h3>ChIP-seq on cells</h3> <p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-1.jpg" alt="Figure 1A" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 1A. The high consistency of the iDeal ChIP-seq kit on the Ion Torrent™ PGM™ (Life Technologies) and GAIIx (Illumina<sup>®</sup>)</strong><br /> ChIP was performed on sheared chromatin from 1 million HelaS3 cells using the iDeal ChIP-seq kit and 1 µg of H3K4me3 positive control antibody. Two different biological samples have been analyzed using two different sequencers - GAIIx (Illumina<sup>®</sup>) and PGM™ (Ion Torrent™). The expected ChIP-seq profile for H3K4me3 on the GAPDH promoter region has been obtained.<br /> Image A shows a several hundred bp along chr12 with high similarity of read distribution despite the radically different sequencers. Image B is a close capture focusing on the GAPDH that shows that even the peak structure is similar.</p> <p class="text-center"><strong>Perfect match between ChIP-seq data obtained with the iDeal ChIP-seq workflow and reference dataset</strong></p> <p><img src="https://www.diagenode.com/img/product/kits/perfect-match-between-chipseq-data.png" alt="Figure 1B" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 1B.</strong> The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p> <p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-2.jpg" alt="Figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 2. Efficient and easy chromatin shearing using the Bioruptor<sup>®</sup> and Shearing buffer iS1 from the iDeal ChIP-seq kit</strong><br /> Chromatin from 1 million of Hela cells was sheared using the Bioruptor<sup>®</sup> combined with the Bioruptor<sup>®</sup> Water cooler (Cat No. BioAcc-cool) during 3 rounds of 10 cycles of 30 seconds “ON” / 30 seconds “OFF” at HIGH power setting (position H). Diagenode 1.5 ml TPX tubes (Cat No. M-50001) were used for chromatin shearing. Samples were gently vortexed before and after performing each sonication round (rounds of 10 cycles), followed by a short centrifugation at 4°C to recover the sample volume at the bottom of the tube. The sheared chromatin was then decross-linked as described in the kit manual and analyzed by agarose gel electrophoresis.</p> <p><img src="https://www.diagenode.com/img/product/kits/iDeal-kit-C01010053-figure-3.jpg" alt="Figure 3" style="display: block; margin-left: auto; margin-right: auto;" width="264" height="320" /></p> <p><strong>Figure 3. Validation of ChIP by qPCR: reliable results using Diagenode’s ChIP-seq grade H3K4me3 antibody, isotype control and sets of validated primers</strong><br /> Specific enrichment on positive loci (GAPDH, EIF4A2, c-fos promoter regions) comparing to no enrichment on negative loci (TSH2B promoter region and Myoglobin exon 2) was detected by qPCR. Samples were prepared using the Diagenode iDeal ChIP-seq kit. Diagenode ChIP-seq grade antibody against H3K4me3 and the corresponding isotype control IgG were used for immunoprecipitation. qPCR amplification was performed with sets of validated primers.</p> <h3>ChIP-seq on tissue</h3> <p><img src="https://www.diagenode.com/img/product/kits/ideal-figure-h3k4me3.jpg" alt="Figure 4A" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 4A.</strong> Chromatin Immunoprecipitation has been performed using chromatin from mouse liver tissue, the iDeal ChIP-seq kit for Histones and the Diagenode ChIP-seq-grade H3K4me3 (Cat. No. C15410003) antibody. The IP'd DNA was subsequently analysed on an Illumina® HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in a region surrounding the GAPDH positive control gene.</p> <p><img src="https://www.diagenode.com/img/product/kits/match-of-the-top40-peaks-2.png" alt="Figure 4B" caption="false" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="280" /></p> <p><strong>Figure 4B.</strong> The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>', 'label2' => 'Species, cell lines, tissues tested', 'info2' => '<p>The iDeal ChIP-seq Kit for Histones is compatible with a broad variety of cell lines, tissues and species - some examples are shown below. Other species / cell lines / tissues can be used with this kit.</p> <p><u>Cell lines:</u></p> <p>Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231</p> <p>Pig: Alveolar macrophages</p> <p>Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2</p> <p>Hamster: CHO</p> <p>Other cell lines / species: compatible, not tested</p> <p><u>Tissues</u></p> <p>Bee – brain</p> <p>Daphnia – whole animal</p> <p>Horse – brain, heart, lamina, liver, lung, skeletal muscles, ovary</p> <p>Human – Erwing sarcoma tumor samples</p> <p>Other tissues: compatible, not tested</p> <p>Did you use the iDeal ChIP-seq for Histones Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>', 'label3' => ' Additional solutions compatible with iDeal ChIP-seq Kit for Histones', 'info3' => '<p><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin EasyShear Kit - Ultra Low SDS </a>optimizes chromatin shearing, a critical step for ChIP.</p> <p> The <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex Library Preparation Kit </a>provides easy and optimal library preparation of ChIPed samples.</p> <p><a href="../categories/chip-seq-grade-antibodies">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p> <p> Plus, for our IP-Star Automation users for automated ChIP, check out our <a href="../p/auto-ideal-chip-seq-kit-for-histones-x24-24-rxns">automated</a> version of this kit.</p>', 'format' => '4 chrom. prep./24 IPs', 'catalog_number' => 'C01010051', 'old_catalog_number' => 'AB-001-0024', 'sf_code' => 'C01010051-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '875', 'price_USD' => '1100', 'price_GBP' => '800', 'price_JPY' => '152400', 'price_CNY' => '', 'price_AUD' => '2750', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ideal-chip-seq-kit-x24-24-rxns', 'meta_title' => 'iDeal ChIP-seq kit x24', 'meta_keywords' => '', 'meta_description' => 'iDeal ChIP-seq kit x24', 'modified' => '2023-04-20 16:00:20', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '1839', 'antibody_id' => null, 'name' => 'iDeal ChIP-seq kit for Transcription Factors', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/ideal-chipseq-transcription-factors-x10-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p><span style="font-weight: 400;">Diagenode’s <strong>iDeal ChIP-seq Kit for Transcription Factors</strong> is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish </span><b>ChIP </b><span style="font-weight: 400;">prior to </span><b>Next-Generation Sequencing</b><span style="font-weight: 400;">. This complete solution contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation, and DNA purification. In addition, unlike competing solutions, the kit contains positive and negative control antibodies (CTCF and IgG, respectively) as well as positive and negative control PCR primers pairs (H19 and Myoglobin exon 2, respectively) for your convenience and a guarantee of optimal results. <br /></span></p> <p><span style="font-weight: 400;">The </span><b> iDeal ChIP-seq kit for Transcription Factors </b><span style="font-weight: 400;">is compatible for cells or tissues:</span></p> <table style="width: 419px; margin-left: auto; margin-right: auto;"> <tbody> <tr> <td style="width: 144px;"></td> <td style="width: 267px; text-align: center;"><span style="font-weight: 400;">Amount per IP</span></td> </tr> <tr> <td style="width: 144px;">Cells</td> <td style="width: 267px; text-align: center;"><strong>4,000,000</strong></td> </tr> <tr> <td style="width: 144px;">Tissues</td> <td style="width: 267px; text-align: center;"><strong>30 mg</strong></td> </tr> </tbody> </table> <p><span style="font-weight: 400;">The iDeal ChIP-seq kit is the only kit on the market validated for major sequencing systems. Our expertise in ChIP-seq tools allows reproducible and efficient results every time. </span></p> <p></p> <p></p>', 'label1' => 'Characteristics', 'info1' => '<ul> <li><span style="font-weight: 400;"><strong>Highly optimized protocol</strong> for ChIP-seq from cells and tissues</span></li> <li><span style="font-weight: 400;"><strong>Validated</strong> for <strong>ChIP-seq</strong> with multiple transcription factors and non-histone targets<br /></span></li> <li><span style="font-weight: 400;"><strong>Most complete kit</strong> available (covers all steps, including the control antibodies and primers)<br /></span></li> <li><span style="font-weight: 400;"><strong>Magnetic beads</strong> make ChIP <strong>easy</strong>, <strong>fast</strong> and more <strong>reproducible</strong></span></li> <li><span style="font-weight: 400;">Combination with Diagenode ChIP-seq antibodies provides <strong>high yields</strong> with excellent <strong>specificity</strong> and <strong>sensitivity</strong><br /></span></li> <li><span style="font-weight: 400;">Purified DNA suitable for any downstream application</span></li> <li><span style="font-weight: 400;">Easy-to-follow protocol</span></li> </ul> <p><span style="font-weight: 400;"></span></p> <p> </p> <h3>ChIP-seq on cells</h3> <p><img src="https://www.diagenode.com/img/product/kits/ideal-ctcf-diagenode.jpg" alt="CTCF Diagenode" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 1.</strong> (A) Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade CTCF antibody. The IP'd DNA was subsequently analysed on an Illumina<sup>®</sup> HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in a region surrounding the GAPDH positive control gene.</p> <p><img src="https://www.diagenode.com/img/product/kits/ideal-figure-b-total-diagendoe-peaks.png" alt="CTCF Diagenode" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 1B.</strong> The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p> <p> </p> <p><img src="https://www.diagenode.com/img/product/kits/ideal-TF-chip-seq-A.png" alt="ChIP-seq figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/kits/ideal-TF-chip-seq-B.png" alt="ChIP-seq figure B" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/kits/ideal-TF-chip-seq-C.png" alt="ChIP-seq figure C" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 2.</strong> Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade HDAC1 (A), LSD1 (B) and p53 antibody (C). The IP'd DNA was subsequently analysed on an Illumina<sup>®</sup> Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in regions of chromosome 3 (A), chromosome 12 (B) and chromosome 6 (C) respectively.</p> <p> </p> <h3>ChIP-seq on tissue</h3> <p><img src="https://www.diagenode.com/img/product/kits/ideal-figure-3a.jpg" alt="ChIP-seq figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 3A.</strong> Chromatin Immunoprecipitation has been performed using chromatin from mouse liver tissue, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade CTCF antibody. The IP'd DNA was subsequently analysed on an Illumina® HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. This figure shows the peak distribution in a region surrounding the Vwf positive control gene.</p> <p><img src="https://www.diagenode.com/img/product/kits/match-of-the-top40-peaks.png" alt="Match of the Top40 peaks" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure 3B.</strong> The ChIP-seq dataset from this experiment has been compared with a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>', 'label2' => 'Species, cell lines, tissues tested', 'info2' => '<p>The iDeal ChIP-seq Kit for Transcription Factors is compatible with a broad variety of cell lines, tissues and species - some examples are shown below. Other species / cell lines / tissues can be used with this kit.</p> <p><span style="text-decoration: underline;">Cell lines:</span></p> <p>Human: A549, A673, BT-549, CD4 T, HCC1806, HeLa, HepG2, HFF, HK-GFP-MR, ILC, K562, KYSE-180, LapC4, M14, MCF7, MDA-MB-231, MDA-MB-436, RDES, SKNO1, VCaP, U2-OS, ZR-75-1 </p> <p>Mouse: ESC, NPCs, BZ, GT1-7, acinar cells, HSPCs, Th2 cells, keratinocytes</p> <p>Cattle: pbMEC, <span>MAC-T</span></p> <p><span>Other cell lines / species: compatible, not tested</span></p> <p><span style="text-decoration: underline;">Tissues:</span></p> <p>Mouse: kidney, heart, brain, iris, liver, limbs from E10.5 embryos</p> <p><span>Horse: l</span>iver, brain, heart, lung, skeletal muscle, lamina, ovary</p> <p>Other tissues: compatible, not tested</p> <p><span style="text-decoration: underline;">ChIP on yeast</span></p> <p>The iDeal ChIP-seq kit for TF is compatible with yeast samples. Check out our <strong><a href="https://www.diagenode.com/files/products/kits/Application_Note-ChIP_on_Yeast.pdf">Application Note</a></strong> presenting an optimized detailed protocol for ChIP on yeast.</p> <p></p> <p>Did you use the iDeal ChIP-seq for Transcription Factors Kit on other cell line / tissue / species? <a href="mailto:agnieszka.zelisko@diagenode.com?subject=Species, cell lines, tissues tested with the iDeal ChIP-seq Kit for TF&body=Dear Customer,%0D%0A%0D%0APlease, leave below your feedback about the iDeal ChIP-seq for Transcription Factors (cell / tissue type, species, other information...).%0D%0A%0D%0AThank you for sharing with us your experience !%0D%0A%0D%0ABest regards,%0D%0A%0D%0AAgnieszka Zelisko-Schmidt, PhD">Let us know!</a></p>', 'label3' => 'Additional solutions compatible with iDeal ChIP-seq kit for Transcription Factors', 'info3' => '<p><span style="font-weight: 400;">The</span> <a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns"><span style="font-weight: 400;">Chromatin EasyShear Kit – Low SDS </span></a><span style="font-weight: 400;">is the kit compatible with the iDeal ChIP-seq kit for TF, recommended for the optimization of chromatin shearing, a critical step for ChIP.</span></p> <p><a href="https://www.diagenode.com/en/p/chip-cross-link-gold-600-ul"><span style="font-weight: 400;">ChIP Cross-link Gold</span></a> <span style="font-weight: 400;">should be used in combination with formaldehyde when working with higher order and/or dynamic interactions, for efficient protein-protein fixation.</span></p> <p><span style="font-weight: 400;">For library preparation of immunoprecipitated samples we recommend to use the </span><b> </b><a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq"><span style="font-weight: 400;">MicroPlex Library Preparation Kit</span></a><span style="font-weight: 400;"> - validated for library preparation from picogram inputs.</span></p> <p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies"><span style="font-weight: 400;">ChIP-seq grade antibodies</span></a><span style="font-weight: 400;"> provide high yields with excellent specificity and sensitivity.</span></p> <p><span style="font-weight: 400;">Check the list of available </span><a href="https://www.diagenode.com/en/categories/primer-pairs"><span style="font-weight: 400;">Primer pairs</span></a><span style="font-weight: 400;"> designed for high specificity to specific genomic regions.</span></p> <p><span style="font-weight: 400;">Plus, for our <a href="https://www.diagenode.com/en/categories/ip-star">IP-Star Automation</a> users for automated ChIP, check out our <a href="https://www.diagenode.com/en/p/auto-ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">automated version</a> of this kit.</span></p>', 'format' => '4 chrom. prep./24 IPs', 'catalog_number' => 'C01010055', 'old_catalog_number' => '', 'sf_code' => 'C01010055-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '875', 'price_USD' => '1100', 'price_GBP' => '800', 'price_JPY' => '152400', 'price_CNY' => '', 'price_AUD' => '2750', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns', 'meta_title' => 'iDeal ChIP-seq kit for Transcription Factors x24', 'meta_keywords' => '', 'meta_description' => 'iDeal ChIP-seq kit for Transcription Factors x24', 'modified' => '2023-04-20 16:01:54', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 8 => array( 'id' => '1927', 'antibody_id' => null, 'name' => 'MicroPlex Library Preparation Kit v2 (12 indexes)', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/MicroPlex-Libary-Prep-Kit-v2-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p><span><strong>Specifically optimized for ChIP-seq</strong></span><br /><br /><span>The MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the </span><a href="./true-microchip-kit-x16-16-rxns">True MicroChIP kit</a><span>, it allows for performing ChIP-seq on as few as 10,000 cells. Less input, fewer steps, fewer supplies, faster time to results! </span></p> <p>The MicroPlex v2 kit (Cat. No. C05010012) contains all necessary reagents including single indexes for multiplexing up to 12 samples using single barcoding. For higher multiplexing (using dual indexes) check <a href="https://www.diagenode.com/en/p/microplex-lib-prep-kit-v3-48-rxns">MicroPlex Library Preparation Kits v3</a>.</p>', 'label1' => 'Characteristics', 'info1' => '<ul> <li><strong>1 tube, 2 hours, 3 steps</strong> protocol</li> <li><strong>Input: </strong>50 pg – 50 ng</li> <li><strong>Reduce potential bias</strong> - few PCR amplification cycles needed</li> <li><strong>High sensitivity ChIP-seq</strong> - low PCR duplication rate</li> <li><strong>Great multiplexing flexibility</strong> with 12 barcodes (8 nt) included</li> <li><strong>Validated with the <a href="https://www.diagenode.com/p/sx-8g-ip-star-compact-automated-system-1-unit" title="IP-Star Automated System">IP-Star<sup>®</sup> Automated Platform</a></strong></li> </ul> <h3>How it works</h3> <center><img src="https://www.diagenode.com/img/product/kits/microplex-method-overview-v2.png" /></center> <p style="margin-bottom: 0;"><small><strong>Microplex workflow - protocol with single indexes</strong><br />An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina® NGS platforms using a fast and simple 3-step protocol</small></p> <ul class="accordion" data-accordion="" id="readmore" style="margin-left: 0;"> <li class="accordion-navigation"><a href="#first" style="background: #ffffff; padding: 0rem; margin: 0rem; color: #13b2a2;"><small>Read more about MicroPlex workflow</small></a> <div id="first" class="content"> <p><small><strong>Step 1. Template Preparation</strong> provides efficient repair of the fragmented double-stranded DNA input.</small></p> <p><small>In this step, the DNA is repaired and yields molecules with blunt ends.</small></p> <p><small><strong>Step 2. Library Synthesis.</strong> enables ligation of MicroPlex patented stem- loop adapters.</small></p> <p><small>In the next step, stem-loop adaptors with blocked 5’ ends are ligated with high efficiency to the 5’ end of the genomic DNA, leaving a nick at the 3’ end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.</small></p> <p><small><strong>Step 3. Library Amplification</strong> enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.</small></p> <p><small>In the final step, the 3’ ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.</small></p> <p><small>Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.</small></p> </div> </li> </ul> <p></p> <h3>Reliable detection of enrichments in ChIP-seq</h3> <p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-a.png" alt="Reliable detection of enrichments in ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure A.</strong> ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina<sup>®</sup> Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.</p> <p><img src="https://www.diagenode.com/img/product/kits/microplex-library-prep-kit-figure-b.png" alt="Reliable detection of enrichments in ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><strong>Figure B.</strong> We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.</p>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '12 rxns', 'catalog_number' => 'C05010012', 'old_catalog_number' => 'C05010010', 'sf_code' => 'C05010012-', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '890', 'price_USD' => '1155', 'price_GBP' => '795', 'price_JPY' => '155400', 'price_CNY' => '', 'price_AUD' => '2890', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'microplex-library-preparation-kit-v2-x12-12-indices-12-rxns', 'meta_title' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)', 'meta_keywords' => '', 'meta_description' => 'MicroPlex Library Preparation Kit v2 x12 (12 indices)', 'modified' => '2023-04-20 15:01:16', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 9 => array( 'id' => '1856', 'antibody_id' => null, 'name' => 'True MicroChIP-seq Kit', 'description' => '<p><a href="https://www.diagenode.com/files/products/kits/truemicrochipseq-kit-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p> <p>The <b>True </b><b>MicroChIP-seq</b><b> kit </b>provides a robust ChIP protocol suitable for the investigation of histone modifications within chromatin from as few as <b>10 000 cells</b>, including <b>FACS sorted cells</b>. The kit can be used for chromatin preparation for downstream ChIP-qPCR or ChIP-seq analysis. The <b>complete kit</b> contains everything you need for start-to-finish ChIP including all validated buffers and reagents for chromatin shearing, immunoprecipitation and DNA purification for exceptional <strong>ChIP-qPCR</strong> or <strong>ChIP-seq</strong> results. In addition, positive control antibodies and negative control PCR primers are included for your convenience and assurance of result sensitivity and specificity.</p> <p>The True MicroChIP-seq kit offers unique benefits:</p> <ul> <li>An <b>optimized chromatin preparation </b>protocol compatible with low number of cells (<b>10.000</b>) in combination with the Bioruptor™ shearing device</li> <li>Most <b>complete kit </b>available (covers all steps and includes control antibodies and primers)</li> <li><b>Magnetic beads </b>make ChIP easy, fast, and more reproducible</li> <li>MicroChIP DiaPure columns (included in the kit) enable the <b>maximum recovery </b>of immunoprecipitation DNA suitable for any downstream application</li> <li><b>Excellent </b><b>ChIP</b><b>-seq </b>result when combined with <a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq">MicroPlex</a><a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq"> Library Preparation kit </a>adapted for low input</li> </ul> <p>For fast ChIP-seq on low input – check out Diagenode’s <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µ</a><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">ChIPmentation</a><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns"> for histones</a>.</p> <p><sub>The True MicroChIP-seq kit, Cat. No. C01010132 is an upgraded version of the kit True MicroChIP, Cat. No. C01010130, with the new validated protocols (e.g. FACS sorted cells) and MicroChIP DiaPure columns included in the kit.</sub></p>', 'label1' => 'Characteristics', 'info1' => '<ul> <li><b>Revolutionary:</b> Only 10,000 cells needed for complete ChIP-seq procedure</li> <li><b>Validated on</b> studies for histone marks</li> <li><b>Automated protocol </b>for the IP-Star<sup>®</sup> Compact Automated Platform available</li> </ul> <p></p> <p>The True MicroChIP-seq kit protocol has been optimized for the use of 10,000 - 100,000 cells per immunoprecipitation reaction. Regarding chromatin immunoprecipitation, three protocol variants have been optimized:<br />starting with a batch, starting with an individual sample and starting with the FACS-sorted cells.</p> <div><button id="readmorebtn" style="background-color: #b02736; color: white; border-radius: 5px; border: none; padding: 5px;">Show Workflow</button></div> <p><br /> <img src="https://www.diagenode.com/img/product/kits/workflow-microchip.png" id="workflowchip" class="hidden" width="600px" /></p> <p> <script type="text/javascript">// <![CDATA[ const bouton = document.querySelector('#readmorebtn'); const workflow = document.getElementById('workflowchip'); bouton.addEventListener('click', () => workflow.classList.toggle('hidden')) // ]]></script> </p> <div class="extra-spaced" align="center"></div> <div class="row"> <div class="carrousel" style="background-position: center;"> <div class="container"> <div class="row" style="background: rgba(255,255,255,0.1);"> <div class="large-12 columns truemicro-slider" id="truemicro-slider"> <div> <h3>High efficiency ChIP on 10,000 cells</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img src="https://www.diagenode.com/img/product/kits/true-micro-chip-histone-results.png" width="800px" /></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center> <p><small><strong>Figure 1. </strong>ChIP efficiency on 10,000 cells. ChIP was performed on human Hela cells using the Diagenode antibodies <a href="https://www.diagenode.com/en/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">H3K4me3</a> (Cat. No. C15410003), <a href="https://www.diagenode.com/en/p/h3k27ac-polyclonal-antibody-classic-50-mg-42-ml">H3K27ac</a> (C15410174), <a href="https://www.diagenode.com/en/p/h3k9me3-polyclonal-antibody-classic-50-ug">H3K9me3</a> (C15410056) and <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-classic-50-mg-34-ml">H3K27me3</a> (C15410069). Sheared chromatin from 10,000 cells and 0.1 µg (H3K27ac), 0.25 µg (H3K4me3 and H3K27me3) or 0.5 µg (H3K9me3) of the antibody were used per IP. Corresponding amount of IgG was used as control. Quantitative PCR was performed with primers for corresponding positive and negative loci. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </center></div> </div> <div> <h3>True MicroChIP-seq protocol in a combination with MicroPlex library preparation kit results in reliable and accurate sequencing data</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img src="https://www.diagenode.com/img/product/kits/fig2-truemicro.jpg" alt="True MicroChip results" width="800px" /></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center> <p><small><strong>Figure 2.</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50.000 of K562 cells. ChIP has been performed accordingly to True MicroChIP protocol followed by the library preparation using MicroPlex Library Preparation Kit (C05010001). The above figure shows the peaks from ChIP-seq experiments using the following antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</small></p> </center></div> </div> <div> <h3>Successful chromatin profiling from 10.000 of FACS-sorted cells</h3> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img src="https://www.diagenode.com/img/product/kits/fig3ab-truemicro.jpg" alt="small non coding RNA" width="800px" /></div> <div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center> <p><small><strong>Figure 3.</strong> (A) Integrative genomics viewer (IGV) visualization of ChIP-seq experiments and heatmap 3kb upstream and downstream of the TSS (B) for H3K4me3. ChIP has been performed using 10.000 of FACS-sorted cells (K562) and H3K4me3 antibody (C15410003) accordingly to True MicroChIP protocol followed by the library preparation using MicroPlex Library Preparation Kit (C05010001). Data were compared to ENCODE standards.</small></p> </center></div> </div> </div> </div> </div> </div> </div> <p> <script type="text/javascript">// <![CDATA[ $('.truemicro-slider').slick({ arrows: true, dots: true, autoplay:true, autoplaySpeed: 3000 }); // ]]></script> </p>', 'label2' => 'Additional solutions compatible with the True MicroChIP-seq Kit', 'info2' => '<p><span style="font-weight: 400;">The <a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit – High SDS</a></span><span style="font-weight: 400;"> Recommended for the optimizing chromatin shearing.</span></p> <p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies"><span style="font-weight: 400;">ChIP-seq grade antibodies</span></a><span style="font-weight: 400;"> for high yields, specificity, and sensitivity.</span></p> <p><span style="font-weight: 400;">Check the list of available </span><a href="https://www.diagenode.com/en/categories/primer-pairs"><span style="font-weight: 400;">primer pairs</span></a><span style="font-weight: 400;"> designed for high specificity to specific genomic regions.</span></p> <p><span style="font-weight: 400;">For library preparation of immunoprecipitated samples we recommend to use the </span><b> </b><a href="https://www.diagenode.com/en/categories/library-preparation-for-ChIP-seq"><span style="font-weight: 400;">MicroPlex Library Preparation Kit</span></a><span style="font-weight: 400;"> - validated for library preparation from picogram inputs.</span></p> <p><span style="font-weight: 400;">For IP-Star Automation users, check out the </span><a href="https://www.diagenode.com/en/p/auto-true-microchip-kit-16-rxns"><span style="font-weight: 400;">automated version</span></a><span style="font-weight: 400;"> of this kit.</span></p> <p><span style="font-weight: 400;">Application note: </span><a href="https://www.diagenode.com/files/application_notes/Diagenode_AATI_Joint.pdf"><span style="font-weight: 400;">Best Workflow Practices for ChIP-seq Analysis with Small Samples</span></a></p> <p></p>', 'label3' => 'Species, cell lines, tissues tested', 'info3' => '<p>The True MicroChIP-seq kit is compatible with a broad variety of cell lines, tissues and species - some examples are shown below. Other species / cell lines / tissues can be used with this kit.</p> <p><strong>Cell lines:</strong></p> <p>Bovine: blastocysts,<br />Drosophila: embryos, salivary glands<br />Human: EndoC-ẞH1 cells, HeLa cells, PBMC, urothelial cells<br />Mouse: adipocytes, B cells, blastocysts, pre-B cells, BMDM cells, chondrocytes, embryonic stem cells, KH2 cells, LSK cells, macrophages, MEP cells, microglia, NK cells, oocytes, pancreatic cells, P19Cl6 cells, RPE cells,</p> <p>Other cell lines / species: compatible, not tested</p> <p><strong>Tissues:</strong></p> <p>Horse: adipose tissue</p> <p>Mice: intestine tissue</p> <p>Other tissues: not tested</p>', 'format' => '20 rxns', 'catalog_number' => 'C01010132', 'old_catalog_number' => 'C01010130', 'sf_code' => 'C01010132-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '595', 'price_USD' => '645', 'price_GBP' => '545', 'price_JPY' => '103600', 'price_CNY' => '', 'price_AUD' => '1615', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'true-microchip-kit-x16-16-rxns', 'meta_title' => 'True MicroChIP-seq Kit | Diagenode C01010132', 'meta_keywords' => '', 'meta_description' => 'True MicroChIP-seq Kit provides a robust ChIP protocol suitable for the investigation of histone modifications within chromatin from as few as 10 000 cells, including FACS sorted cells. Compatible with ChIP-qPCR as well as ChIP-seq.', 'modified' => '2023-04-20 16:06:10', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 10 => array( 'id' => '2837', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico DNA QC kit', 'description' => '<p><span>We have developed the DNA Quality Control Kit for you to be able to track the efficiency of your <strong>Bioruptor</strong></span><span><strong>® Pico</strong> </span><span>and to figure out the right time for servicing. After the sonication, if your QC does not reach our standards, please, contact Diagenode for recommendations. However, if your QC passes, we wish you every success with your Bioruptor</span><span>®</span><span>.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'C40010002', 'old_catalog_number' => '', 'sf_code' => 'C40010002-', 'type' => 'REF', 'search_order' => '01-Accessory', 'price_EUR' => '120', 'price_USD' => '130', 'price_GBP' => '110', 'price_JPY' => '17700', 'price_CNY' => '', 'price_AUD' => '325', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'Bioruptor-Pico-DNA-QC-kit', 'meta_title' => 'Bioruptor® Pico DNA QC kit', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico DNA QC kit', 'modified' => '2020-11-17 11:26:47', 'created' => '2016-09-21 17:04:22', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 11 => array( 'id' => '2952', 'antibody_id' => null, 'name' => '0.2 ml microtubes for Bioruptor® Pico', 'description' => '<p><span>The 0.2 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico and are strongly recommended for DNA and chromatin shearing applications using this instrument. Recommended sample volume: 20-100 μl.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '1000 pc', 'catalog_number' => 'C30010020', 'old_catalog_number' => '', 'sf_code' => 'C30010020-1863', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '360', 'price_USD' => '450', 'price_GBP' => '370', 'price_JPY' => '52900', 'price_CNY' => '0', 'price_AUD' => '1125', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => '02ml-microtubes-for-bioruptor-pico', 'meta_title' => '0.2 ml microtubes for Bioruptor® Pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-25 03:53:19', 'created' => '2018-02-12 15:41:23', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ) ), 'Application' => array( (int) 0 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'position' => '10', 'parent_id' => '3', 'name' => 'FFPE DNA extraction', 'description' => '<div class="row"> <div class="large-12 columns">Diagenode's high yields FFPE DNA extraction using Bioruptor<sup><span>®</span></sup> is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by traditional methods or can be sheared with the Bioruptor<sup>®</sup> Pico ultrasonicator for downstream NGS library prep using the MicroPlex Library Preparation Kit.</div> <div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/ffpe_workflow.png" /></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'ffpe-dna-extraction', 'meta_keywords' => 'FFPE DNA extraction,Next-Gen Sequencing,Bioruptor® ultrasonicator', 'meta_description' => 'Diagenode's high yields FFPE DNA extraction using Bioruptor is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no tox', 'meta_title' => 'FFPE DNA extraction using Bioruptor® ultrasonicator | Diagenode', 'modified' => '2017-10-16 14:34:57', 'created' => '2014-10-01 01:24:40', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3', 'position' => '10', 'parent_id' => null, 'name' => '次世代シーケンシング', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化：NGSのワンストップショップ</h2> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-12 medium-12 large-12 columns"> <h4>1. 断片化装置を選択してください：150 bp〜75 kbの範囲でDNAを断片化します。</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns">5μlまで断片化：150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td> <td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td> <td class="small-4 medium-4 large-4 columns">20または50μlの断片化が可能です。</td> </tr> </tbody> </table> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-8 medium-8 large-8 columns"> <h4>2. 最適化されたライブラリー調整キットを選択してください。</h4> </th> <th class="small-4 medium-4 large-4 columns"> <h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力：MicroPlex Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上：iDeal Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td> </tr> </tbody> </table> </div> </div> <blockquote> <div class="row"> <div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span> <p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5μlのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p> <ul> <li>信頼されるせん断装置</li> <li>様々なインプットからのライブラリ作成キット</li> <li>独自の自動化デバイス</li> </ul> </div> </div> </blockquote> <div class="row"> <div class="small-12 columns"> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a> <div id="panel1a" class="content"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong>次世代シーケンシング (NGS)</strong> ）は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p> <p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p> <p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p> </div> </div> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <p><strong>NGS アプリケーション</strong></p> <ul> <li>全ゲノム配列決定</li> <li>デノボシーケンシング</li> <li>標的配列</li> <li>Exomeシーケンシング</li> <li>トランスクリプトーム配列決定</li> <li>ゲノム配列決定</li> <li>ミトコンドリア配列決定</li> <li>DNA-タンパク質相互作用（ChIP-seq</li> <li>バリアント検出</li> <li>ゲノム仕上げ</li> </ul> </div> <div class="small-6 medium-6 large-6 columns"> <p><strong>研究分野におけるNGS:</strong></p> <ul> <li>腫瘍学</li> <li>リプロダクティブ・ヘルス</li> <li>法医学ゲノミクス</li> <li>アグリゲノミックス</li> <li>複雑な病気</li> <li>微生物ゲノミクス</li> <li>食品・環境ゲノミクス</li> <li>創薬ゲノミクス - パーソナライズド・メディカル</li> </ul> </div> <div class="small-12 medium-12 large-12 columns"> <p><strong>NGSの用語</strong></p> <dl> <dt>リード（読み取り）</dt> <dd>この装置から得られた連続した単一のストレッチ</dd> <dt>断片リード</dt> <dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd> <dt>断片ペアエンドリード</dt> <dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd> <dt>メイトペアリード</dt> <dd>大きなDNA断片（通常は予め定義されたサイズ範囲）の各末端から2つの読み取り。</dd> <dt>カバレッジ（例）</dt> <dd>30×適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd> </dl> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2>NGSプラットフォーム</h2> <h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3> <p>イルミナは、クローン的に増幅された鋳型DNA（クラスター）上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド（それぞれ異なる蛍光色素で標識された）の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p> <h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3> <p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR（emPCR）を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p> <h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3> <p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム（SMRT）シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA（dsDNA）断片が、Megaruptor（登録商標）のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p> <h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3> <p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔（ナノ細孔）またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド（AまたはG r CまたはT）ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor（登録商標）を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p> <h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3> <p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR（emPCR）を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p> <h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3> <p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅（emPCR）で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p> </div> </div> </div> </li> </ul> </div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'next-generation-sequencing', 'meta_keywords' => 'Next-generation sequencing,NGS,Whole genome sequencing,NGS platforms,DNA/RNA shearing', 'meta_description' => 'Diagenode offers kits and DNA/RNA shearing technology prior to next-generation sequencing, many Next-generation sequencing platforms require preparation of the DNA.', 'meta_title' => 'Next-generation sequencing (NGS) Applications and Methods | Diagenode', 'modified' => '2018-07-26 05:24:29', 'created' => '2015-04-01 22:47:04', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '9', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-seq', 'description' => '<div class="row"> <div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div> <div class="large-12 columns"></div> <h5 class="large-12 columns"><strong></strong></h5> <h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div> <div class="large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by sonication to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with <strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: Reverse cross-links, elute, and purify </li> <li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li> <li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img alt="" src="https://www.diagenode.com/img/banners/banner-customizer.png" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chromatin-immunoprecipitation-sequencing', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers wide range of kits and antibodies for Chromatin Immunoprecipitation Sequencing (ChIP-Seq) and also provides Bioruptor for chromatin shearing', 'meta_title' => 'Chromatin Immunoprecipitation - ChIP-seq Kits - Dna methylation | Diagenode', 'modified' => '2017-11-14 09:57:16', 'created' => '2015-04-12 18:08:46', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'Protein extraction', 'description' => '<div class="row"> <div class="large-12 columns">Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div> </div> <p></p> <div class="row"> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" /> <p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p> </div> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" /> <p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'protein-extraction', 'meta_keywords' => 'Protein extraction,Bioruptor,Sonication,Protein Analysis', 'meta_description' => 'Diagenode provides efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Bioruptor® deliver high quality protein.', 'meta_title' => 'Protein extraction using Bioruptor® Sonication device | Diagenode', 'modified' => '2017-10-16 14:39:42', 'created' => '2014-07-02 04:41:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '6', 'position' => '10', 'parent_id' => '1', 'name' => 'メチル化DNA結合タンパク質', 'description' => '<div class="row"> <div class="large-12 columns">MBD方法は、メチル化DNAに対するH6-GST-MBD融合タンパク質の非常に高い親和性に基づいています。 このタンパク質は、N末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）を含有します。 このH6-GST-MBD融合タンパク質を用いて、メチル化CpGを含むDNAを特異的に単離する事が可能です。<br /><br />DiagenodeのMethylCap®キットは、二本鎖DNAの高濃縮と、メチル化CpG密度の関数における微分分画を可能にします。 分画はサンプルの複雑さを軽減し、次世代のシーケンシングを容易にします。 MethylCapアッセイに先立ち、DNAを最初に抽出し、Picoruptorソニケーターを用いて断片化します。<br /> <h3>概要</h3> <p class="text-center"><br /><img src="https://www.diagenode.com/img/applications/methyl_binding_domain_overview.jpg" /></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'methylbinding-domain-protein', 'meta_keywords' => 'Epigenetic,Methylbinding Domain Protein,MBD,DNA methylation,DNA replication,MethylCap,MethylCap assay,', 'meta_description' => 'Methylbinding Domain Protein(MBD) approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase', 'meta_title' => 'Epigenetic Methylbinding Domain Protein(MBD) - DNA methylation | Diagenode', 'modified' => '2019-03-22 12:32:12', 'created' => '2015-06-02 17:05:42', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2', 'position' => '10', 'parent_id' => null, 'name' => 'エピジェネティクス・クロマチン解析', 'description' => '<div class="row"> <div class="large-12 columns">エピジェネティクス研究は、異なる転写パターン、遺伝子発現およびサイレンシングを引き起こすクロマチンの変化に対処します。<br /><br />クロマチンの主成分はDNA<span>およびヒストン蛋白質です。<span> </span></span>各ヒストンコア蛋白質（H2A<span>、</span>H2B<span>、</span>H3<span>および</span>H4<span>）の</span>2<span>つのコピーを</span>8<span>量体に組み込み、</span>DNA<span>で包んでヌクレオソームコアを形成させます。<span> </span></span>ヌクレオソームは、転写機械のDNA<span>への接近可能性および</span>クロマチン再構成因子を制御します。</div> <div class="large-12 columns"> <p></p> <p>クロマチン免疫沈降（ChIP<span>）は、関心対象の特定の蛋白質に対するゲノム結合部位の位置を解明するために使用される方法であり、遺伝子発現の制御に関する非常に貴重な洞察を提供します。<span> </span></span>ChIPは特定の抗原を含むクロマチン断片の選択的富化に関与します。 特定の蛋白質または蛋白質修飾を認識する抗体を使用して、特定の遺伝子座における抗原の相対存在量を決定します。</p> <p>ChIP-seq<span>および</span>ChIP-qPCR<span>は、蛋白質</span>-DNA<span>結合部位の同定を可能にする技術です。</span></p> <p> </p> </div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'epigenetics-chromatin-study', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenodeは、ChIP-Seqおよびクロマチン免疫沈降（ChIP）専用キットと抗体を定量PCRアプリケーションと組み合わせて幅広く提供しています。', 'meta_title' => 'エピジェネティクス - クロマチン免疫沈降（ChIP）| Diagenode', 'modified' => '2018-01-11 09:58:47', 'created' => '2015-02-20 16:10:25', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '6', 'position' => '1', 'parent_id' => '1', 'name' => 'Bioruptor<sup>®</sup>', 'description' => '<div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</span></p> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/TAB-BR-comparaison.pdf" target="_blank"><img src="https://www.diagenode.com/img/bouton-comparaison.png" /></a></p> </div> <center> <div class="small-12 medium-4 large-4 columns"><a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v" target="_blank"><img src="https://www.diagenode.com/img/epicafe-jointhecommunity.png" width="70%" /></a></div> </center></div> <p><span></span></p> <div class="content_block"> <div class="centered row"> <h2 style="text-align: center;"></h2> <h2 style="text-align: center;">Technology explained</h2> <div class="container-wrapper-genially" style="position: relative; min-height: 400px; max-width: 80%; margin: 0 auto;"><video width="300" height="150" style="position: absolute; top: 45%; left: 50%; transform: translate(-50%, -50%); width: 80px; height: 80px; margin-bottom: 10%;" class="loader-genially" autoplay="autoplay" loop="loop" playsinline="playsInline" muted="muted"><source src="https://static.genial.ly/resources/panel-loader-low.mp4" type="video/mp4" />Your browser does not support the video tag.</video> <div id="601970a2edea170d2af29118" class="genially-embed" style="margin: 0px auto; position: relative; height: auto; width: 100%;"></div> </div> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script>// <![CDATA[ (function (d) { var js, id = "genially-embed-js", ref = d.getElementsByTagName("script")[0]; if (d.getElementById(id)) { return; } js = d.createElement("script"); js.id = id; js.async = true; js.src = "https://view.genial.ly/static/embed/embed.js"; ref.parentNode.insertBefore(js, ref); }(document)); // ]]></script> </div> </div> <p><span> <br /></span></p> <div class="spacer"></div> <div class="content_block"> <div class="centered row"> <h2 style="text-align: center;">Reproductibility is our priority</h2> </div> </div> <div><img src="https://www.diagenode.com/img/shearing/reproductibility.png" alt="reproductibility" /> <p class="bottom_note"></p> </div> <div class="content_block"> <div class="centered row"> <h2 style="text-align: center;">An affordable instrument for wide range of applications</h2> </div> </div> <p style="text-align: center;">Designed for any researchers, the Bioruptor gives the user the right level of flexibility.</p> <table style="width: 972px;"> <tbody> <tr style="height: 56px;"> <th style="width: 380px; height: 56px;"></th> <th class="text-center" style="width: 126px; height: 56px;">Bioruptor</th> <th class="text-center" style="width: 141px; height: 56px;">Cup Horn Sonicators</th> <th class="text-center" style="width: 156px; height: 56px;">Focused <br />ultra-sonicators</th> <th class="text-center" style="width: 155px; height: 56px;">Multi Sample Sonicator</th> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Instrument pricing</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Consumables pricing</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Range of applications</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Scalable and sample volume flexibility</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i></center></td> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Throughput</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> </tr> </tbody> </table> <div class="content_block"> <div class="centered row"> <h2 style="text-align: center;"></h2> <h2 style="text-align: center;">Bioruptor ultrasonication for best results in:</h2> <p><b><span>✓ Chromatin shearing</span><span> </span><span style="font-weight: 400;">- Industry leader in accurate and tight fragment ranges</span></b></p> <p><b><span>✓ DNA shearing</span><span> </span><span style="font-weight: 400;">- Excellent results for optimal fragment lengths in NGS library prep</span></b></p> <p><b><span>✓<span> </span></span>Protein aggregation studies </b><span style="font-weight: 400;">- Standardizing seeding with the robust Bioruptor.<br /></span><i><span style="font-weight: 400;">Read the app note by Dr. Kelvin Luk at the University of Pennsylvania </span></i><a href="https://www.diagenode.com/en/documents/standardizing-seeding-experiments-for-the-understanding-of-parkinson-disease" style="color: #13b29c;"><i><span style="font-weight: 400;">“Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology”</span></i></a></p> <p><b><b><span>✓<span> </span></span></b>3D genome analysis with Hi-C</b><span style="font-weight: 400;"> - Preparing chromatin libraries with high-quality sonication.<br /></span><i><span style="font-weight: 400;">Read the app note, “</span></i><a href="https://www.diagenode.com/en/documents/applicationnote-arima-low-input" style="color: #13b29c;"><i><span style="font-weight: 400;">Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit”</span></i></a></p> <p><b><b><span>✓<span> </span></span></b>Mass spectrometry</b> <b>and increasing protein identification</b><span style="font-weight: 400;">- Sample preparation using Preomics iST and Bioruptor sonication.<br /></span><i><span style="font-weight: 400;">Read the app note “</span></i><a href="https://www.diagenode.com/en/documents/wp-ist-adaptators" style="color: #13b29c;"><i><span style="font-weight: 400;">Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder”</span></i></a></p> <p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>Cell lysis, liposome prep, protein extraction, RNA extraction and more</b></span></p> <p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>CUT&RUN –Sonication of input DNA (for enrichment comparison) for NGS</b></span></p> </div> </div> <p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p> <p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-shearing-device', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor,ultrasonicator devices,probe sonicator,Next-Generation Sequencing', 'meta_description' => 'Bioruptor Sonication is ideal for Chromatin Shearing for Chromatin Immunoprecipitation (ChIP), Genomic DNA Shearing for next Generation Sequencing, RNA Shearing, Cell and Tissue Disruption', 'meta_title' => 'Bioruptor Sonication for Chromatin, DNA / RNA Shearing, Cell and Tissue Disruption | Diagenode', 'modified' => '2023-04-26 16:00:39', 'created' => '2014-12-18 22:08:39', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '35', 'name' => 'Bioruptor ® Sonicator Brochure', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup>®</sup> <span>uses state-of-the-art shearing device to disrupt, disperse, or shear a variety of sample types for biological, chemical, pharmaceutical, and industrial applications. It is<span> t</span></span><span>he standard for DNA and RNA shearing, chromatin shearing, cell and tissue disruption, FFPE DNA extraction, DNA methylation studies and tissue RNA extraction. </span></p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Bioruptor_Sonicator_Brochure.pdf', 'slug' => 'bioruptor-sonicator-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-01-29 09:09:15', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '18', 'name' => 'Bioruptor® DNA shearing for Agilent SureSelect library preparation: a perfect combination', 'description' => '<p><span>The Cologne Center for Genomics (CCG), headed by Prof. Dr. Peter Nürnberg,</span><span> shows that the Bioruptor</span><span>® </span><span>NGS is an ideal device for mid- to high-throughput NGS labs as it produces reliable and reproducible results in all library preparation protocols that need DNA fragmentation. </span></p>', 'image_id' => null, 'type' => 'Application note', 'url' => 'files/application_notes/Bioruptor_DNA_shearing_for_Agilent_SureSelectlibrary_prep.pdf', 'slug' => 'bioruptor-dna-shearing-for-agilent-sureselectlibrary-prep', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-05-18 15:40:23', 'created' => '2015-07-03 16:05:20', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '42', 'name' => 'Bioruptor<sup>®</sup> NGS: Unbiased DNA shearing for Next-Generation Sequencing', 'description' => '<p><span>Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center, University of Liège (Belgium). The center routinely performs a variety of Next-Generation Sequencing (NGS) applications including de novo sequencing, whole genome and amplicon sequencing, and targeted resequencing on an Illumina GAII</span><span>x </span><span>analyzer. Coppieters recognizes that optimal sequencing results rely heavily on the quality and preparation of DNA libraries. Library preparation can be complex with multiple steps that require optimization. As Coppieters notes, “A prerequisite to obtaining high quality Next-Generation Sequencing data is to have libraries that have the desired insert size (November 2011)”. </span></p>', 'image_id' => null, 'type' => 'Case study', 'url' => 'files/case_studies/CaseStudy-Bioruptor.pdf', 'slug' => 'casestudy-bioruptor', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-29 15:45:29', 'created' => '2015-07-03 16:05:36', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '19', 'name' => 'True MicroChIP and MicroPlex kits', 'description' => '<p><span>From minuscule amounts to magnificent results: reliable ChIP-seq data from 10,000 cells with the True MicroChIP</span>™ <span>and the MicroPlex Library Preparation</span>™ <span>kits. </span></p>', 'image_id' => null, 'type' => 'Application note', 'url' => 'files/application_notes/True_MicroChIP_and_MicroPlex_kits_Application_Note.pdf', 'slug' => 'true-microchip-and-microplex-kits-application-note', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-05-18 11:36:11', 'created' => '2015-07-03 16:05:20', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '24', 'name' => 'ChIP-seq from human tumor tissue', 'description' => '<p style="text-align: justify;"><span>Application of the Bioruptor</span><sup>®</sup> <span>Pico for shearing chromatin fragments for ChIP-seq from human tumor tissue.</span></p> <p style="text-align: justify;"><span>Over the last several years, chromatin immunoprecipitation followed by massive parallel sequencing (ChIP-seq) has helped to identify transcription factor function and epigenetic modifications on a genome-wide scale. Most of these studies have classically been performed using cell line material. In this application note, we illustrate that the Bioruptor</span><sup>®</sup> <span>Pico (Diagenode) can process various human tumor tissue for ChIP-seq analyses of transcription factors as well as epigenetic marks. </span></p>', 'image_id' => null, 'type' => 'Application note', 'url' => 'files/application_notes/ChIPseq_from_human_tumor_tissue.pdf', 'slug' => 'chipseq-from-human-tumor-tissue', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:06:42', 'created' => '2015-07-03 16:05:20', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '16', 'name' => 'ChIP kit results with True MicroChIP kit', 'description' => '<p style="text-align: justify;"><span>Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become the gold standard for whole-genome mapping of protein-DNA interactions. However, conventional ChIP protocols require abundant amounts of starting material (at least hundreds of thousands of cells per immunoprecipitation) limiting the application for the ChIP technology to few cell samples. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/ChIP_kit_results_with_True_MicroChIP_kit_Poster.pdf', 'slug' => 'chip-kit-results-with-true-microchip-kit-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:09:25', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '7', 'name' => 'Bioruptor<sup>®</sup> NGS System – Designed Exclusively for Fast & Accurate DNA Shearing', 'description' => '<p><span>The Bioruptor</span><sup>®</sup><span>NGS is the DNA shearing device of choice for sequencing applications, providing optimal yields, lengths, and consistency. Different fragment size ranges are frequently required for downstream applications (e.g. bridge amplification) for sequencing. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Fast_Accurate_DNA_Shearing_with_Bioruptor_NGS _Poster.pdf', 'slug' => 'fast-accurate-dna-shearing-with-bioruptor-ngs-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-07 15:08:37', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 8 => array( 'id' => '897', 'name' => 'Application of the Bioruptor® Pico for best shearing of yeast genomic DNA for NGS', 'description' => '<p>DNA shearing is important step for next-generation sequencing library preparation. Important is reproducibility and ef ciency of shearing. <i>S. cerevisiae</i> is yeast and have smaller amount of DNA per cells. They have a cell wall so the conditions which we use for the genomic DNA from yeast are different than for mammalian cells.</p>', 'image_id' => null, 'type' => 'Application note', 'url' => 'files/application_notes/application_note-pico-shearingdna-03_16.pdf', 'slug' => 'application-note-pico-shearing-dna-for-ngs', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-04-10 10:15:18', 'created' => '2016-04-06 14:20:14', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 9 => array( 'id' => '961', 'name' => 'DNA shearing for Next-Generation Sequencing with the Bioruptor® pico, 0.2 ml Bioruptor® microtubes and the corresponding holder', 'description' => '<p>Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, among others the precise DNA fragmentation. The Diagenode Bioruptor® Pico ensures that the DNA is efficiently sheared to an appropriate and consistent fragment size. This first step is critical to generate high quality unbiaised libraries.</p>', 'image_id' => '246', 'type' => 'Protocol', 'url' => 'files/protocols/protocol-dna-shearing-tubes-02ml.pdf', 'slug' => 'protocol-dna-shearing-tubes-02ml', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-06-07 10:38:09', 'created' => '2017-06-02 11:11:06', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 10 => array( 'id' => '960', 'name' => 'Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder', 'description' => '<p>The first critical step of a successful ChIP experiment is the preparation of sheared chromatin with a suitable fragment range which best represents the biological scenario being investigated. The Bioruptor<sup>®</sup> Pico ensures that the chromatin is efficiently sheared to an appropriate and consistent fragment size. Bioruptor® shearing occurs in a temperature-controlled environment providing a high quality chromatin with preserved epitopes suitable for ChIP-qPCR and ChIP-seq experiments.</p>', 'image_id' => '246', 'type' => 'Protocol', 'url' => 'files/protocols/chIP-shearing-tubes02ml-protocol.pdf', 'slug' => 'chromatin-shearing-with-the-bioruptor-pico-02-ml-bioruptor-microtubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-06-07 10:38:44', 'created' => '2017-06-01 17:54:08', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 11 => array( 'id' => '963', 'name' => 'Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing', 'description' => '<p>Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation of most archived pathological specimens. Archival FFPE samples provide an invaluable repository of information for genetic analysis. These samples can be analyzed by next-generation sequencing for applications including biomarker discovery, drug development, and cancer research. However, DNA from archival tissues is challenging to use in NGS studies due to insufficient quality, including degradation, chemical modifications and the presence of single-stranded molecules.</p>', 'image_id' => '247', 'type' => 'Application note', 'url' => 'files/application_notes/AN-FFPE-07_2017.pdf', 'slug' => 'workflow-fo-ffpe-derived-dna-note', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-07-12 17:10:51', 'created' => '2017-07-12 17:10:51', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 12 => array( 'id' => '973', 'name' => 'Efficient and streamlined solution for high-quality NGS library preparation using the Bioruptor® Pico and JetSeqTM Flex DNA Library Preparation Kit', 'description' => '<p>Next Generation Sequencing workflows include a number of critical steps which can have a significant impact both on the quality of the results and on the overall cost of the project. In particular, DNA fragmentation and library preparation, as the first two steps of the workflow, are crucial, since at these early stages significant bias can be introduced to the sample, compromising the quality of the datasets obtained later. In this application note we describe how a fully functional, high-quality library for Illumina platforms has been prepared, using the Diagenode Bioruptor® Pico and the Bioline JetSeq™ Flex DNA Library Preparation Kit.</p>', 'image_id' => '247', 'type' => 'Application note', 'url' => 'files/application_notes/AN-BIOLINE-08_2017.pdf', 'slug' => 'bioline-application-note', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-11-02 16:25:19', 'created' => '2017-11-02 16:22:58', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 13 => array( 'id' => '975', 'name' => 'Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico', 'description' => '<p>The chromatin shearing is very important step for all kind of ChIP analysis followed by next-generation sequencing. The efficiency, reproducibility and straightforwardness are the most important characteristics for shearing. The using of S. cerevisiae as a model organism is even more complicated because they have a cell wall which require special lysis and can effect shearing. In this protocol we compared the yeast chromatin shearing by Covaris™ S2 and Bioruptor® Pico, and the chromatin crosslinking by 16% methanol-free and 37% methanol-containing formaldehyde.</p>', 'image_id' => '247', 'type' => 'Application note', 'url' => 'files/application_notes/ComparisonCovarisBioruptor.pdf', 'slug' => 'comparison-covaris-bioruptor', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-12-14 16:50:51', 'created' => '2017-12-14 16:50:51', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 14 => array( 'id' => '989', 'name' => 'Ultrasensitive proteomics for microdissected fresh frozen tissue samples', 'description' => '<p>This protocol, specifically designed for ultrasensitive proteomics, enables high throughput quantitative proteomics study on microdissected tissue samples. The Bioruptor<sup>®</sup> Pico provided high efficiency protein extraction, essential for the analysis of proteins from micro-scale tissue samples.</p>', 'image_id' => '247', 'type' => 'Application note', 'url' => 'files/application_notes/AN-PICO-MASSSPEC-10_2017.pdf', 'slug' => 'ultrasensitive-proteomics-for-microdissected-fresh-frozen-tissue-samples', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-24 11:01:17', 'created' => '2018-01-24 11:01:17', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 15 => array( 'id' => '992', 'name' => 'Bioruptor® Pico Sonication System Manual', 'description' => '<p>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system optimized for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. The built-in cooling system (Bioruptor® Cooler) ensures high precision temperature control resulting in higher quality samples.</p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_pico_cooler_manual.pdf', 'slug' => 'bioruptor_pico_cooler_manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-02-23 17:53:11', 'created' => '2018-02-14 16:52:18', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 16 => array( 'id' => '1057', 'name' => 'Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology', 'description' => '<p>Dr. Kelvin Luk’s lab at the University of Pennsylvania focuses on understanding Parkinson’s disease (PD), a progressive neurodegenerative condition for which there is currently no cure. Their current efforts focus on the biology of midbrain dopamine neurons, PD drug discovery, and the role of protein misfolding in PD. We have demonstrated that the Bioruptor® provides a number of benefits in standardizing and creating representative seeding material that can serve to nucleate inclusions in neurons to better understand protein aggregation and misfolding models in α-synuclein.</p>', 'image_id' => '247', 'type' => 'Application note', 'url' => 'files/application_notes/AN-Standardizing-seeding-experiments-for-the-understanding-of-Parkinson-disease-Kelvin-Luk-07_2019.pdf', 'slug' => 'standardizing-seeding-experiments-for-the-understanding-of-parkinson-disease', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-07-18 15:57:11', 'created' => '2019-07-18 15:53:18', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array( (int) 0 => array( 'id' => '4', 'name' => 'Temperature-controlled', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 11:49:49', 'created' => '2014-12-22 03:33:31', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '5', 'name' => 'Easy to use', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 11:50:14', 'created' => '2014-11-11 14:16:51', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '6', 'name' => 'All-in-one solution', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 11:50:41', 'created' => '2014-06-21 12:07:09', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '7', 'name' => 'Processing of 6-16 samples', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-03-13 11:10:21', 'created' => '2014-11-09 09:21:21', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '9', 'name' => 'Sample size 5 µl – 2 ml', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 11:57:08', 'created' => '2014-07-13 15:46:55', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '13', 'name' => 'No soundproof box', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 11:59:59', 'created' => '2014-07-23 08:44:32', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '41', 'name' => 'Five year warranty', 'slug' => '5-year-warranty', 'meta_keywords' => 'Five year warranty', 'meta_description' => 'Five year warranty', 'modified' => '2020-01-21 15:48:38', 'created' => '2020-01-21 15:48:38', 'ProductsFeature' => array( [maximum depth reached] ) ) ), 'Image' => array( (int) 0 => array( 'id' => '133', 'name' => 'product/shearing_technologies/bioruptor_pico.jpg', 'alt' => 'Bioruptor pico next gen sequencing ', 'modified' => '2020-11-27 10:00:30', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1772', 'name' => 'product/shearing_technologies/B010600010.jpg', 'alt' => 'B010600010', 'modified' => '2018-02-14 15:41:46', 'created' => '2018-02-14 15:41:46', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1773', 'name' => 'product/shearing_technologies/B010600010-2.jpg', 'alt' => 'B010600010', 'modified' => '2018-02-14 16:18:03', 'created' => '2018-02-14 15:42:00', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1774', 'name' => 'product/shearing_technologies/B01060010-bioruptor-pico-cooler.jpg', 'alt' => 'Pico + cooler', 'modified' => '2018-03-06 11:01:33', 'created' => '2018-03-06 11:01:33', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '37', 'name' => 'Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in cells and tissues. The assay can be cumbersome and the success is highly dependent on the quality of chromatin. The first critical step of a successful ChIP experiment is the preparation of sheared chromatin which is representative of the biological scenario of interest. </span></p>', 'image_id' => '224', 'type' => 'Protocol', 'url' => 'files/protocols/bioruptor-pico-chromatin-preparation-guide.pdf', 'slug' => 'bioruptor-pico-chromatin-preparation-guide', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-06-02 11:15:33', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1', 'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico', 'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>', 'image_id' => '222', 'type' => 'Protocol', 'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf', 'slug' => 'chromatin-shearing-from-tissue-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-29 22:10:32', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl', 'description' => '<p><span>HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleus isolation is performed using buffers and reagents of Diagenode’s Chromatin Shearing Optimization kit - Low SDS (Cat. No. AA-001-0100) 1x10e6 cells are then resuspended in 100 μl Shearing Buffer prior to chromatin shearing. </span></p>', 'image_id' => '208', 'type' => 'Protocol', 'url' => 'files/protocols/Consistent_and_highly_reproducible_chromatin_shearing_with_the_Bioruptor_Pico_10ul_300ul.pdf', 'slug' => 'consistent-and-highly-reproducible-chromatin-shearing-with-the-bioruptor-pico-10ul-300ul', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:42:59', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '4', 'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml', 'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>', 'image_id' => '212', 'type' => 'Protocol', 'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf', 'slug' => '15ml-sonication-tube-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-19 13:51:19', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '65', 'name' => 'Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder', 'description' => '<p>The first critical step of a successful ChIP experiment is the preparation of sheared chromatin with a suitable fragment range which best represents the biological scenario being investigated. The Bioruptor<sup>®</sup> Pico ensures that the chromatin is efficiently sheared to an appropriate and consistent fragment size. Bioruptor® shearing occurs in a temperature-controlled environment providing a high quality chromatin with preserved epitopes suitable for ChIP-qPCR and ChIP-seq experiments.</p>', 'image_id' => '246', 'type' => 'Protocol', 'url' => 'files/protocols/chIP-shearing-tubes02ml-protocol.pdf', 'slug' => 'chromatin-shearing-with-the-bioruptor-pico-02-ml-bioruptor-microtubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-06-07 10:41:07', 'created' => '2017-06-01 17:54:08', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '60', 'name' => 'Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor® ', 'description' => '<p><span>Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene, or a mineral oil-based method which can be time consuming and messy. Claire Josse and colleagues from the Human Genetic Laboratory at the GIGA - University of Liège have developed a new protocol combining the Bioruptor</span><span>® </span><span>Pico with the AllPrep FFPE DNA/RNA kit from Qiagen</span><span>® </span><span>to ef ciently prepare FFPE samples for Next-Generation Sequencing. The Bioruptor</span><span>® </span><span>Pico removes the parafin and rehydrates the tissue in just one solvent-free step followed by a mild crosslink reversal to preserve DNA and RNA integrity. The nucleic acids are then extracted with the AllPrep FFPE DNA/RNA kit and sheared using the Bioruptor</span><span>® </span><span>Pico to the desired length for sequencing library preparation. </span></p>', 'image_id' => '246', 'type' => 'Protocol', 'url' => 'files/protocols/bioruptor-deparaffinization.pdf', 'slug' => 'bioruptor-deparaffinization-protocol', 'meta_keywords' => 'bioruptor-deparaffinization-protocol', 'meta_description' => 'bioruptor-deparaffinization-protocol', 'modified' => '2016-04-29 17:49:23', 'created' => '2015-11-18 16:29:42', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '7', 'name' => 'DNA Shearing for Bioruptor<sup>®</sup> Pico (2013-2019) - Standard 100 μl Volume', 'description' => '<p><span>Standard protocols for programmable DNA size distributions, excellent reproducibility, and high dsDNA yields with Bioruptor</span><span>® </span><span>Pico.</span></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'image_id' => '223', 'type' => 'Protocol', 'url' => 'files/protocols/Standard_protocols_for_DNAShearing.pdf', 'slug' => 'standard-protocols-for-dnashearing', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-07-31 12:15:46', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '25', 'name' => 'DNA Shearing for Bioruptor® Pico (2013-2019) - 50 μl Volume ', 'description' => '<p>For DNA shearing in 50 μl we highly recommend to use the <a href="../../p/0-1-ml-tube-holder-tube-adaptors-for-bioruptor-pico-1-pack">0.1 ml tube holder & tube adaptors (Cat. No. B01200041)</a> and the corresponding <a href="../../p/0-1-ml-bioruptor-microtubes-120-tubes">0.1 ml Bioruptor<span>® </span><span>Microtubes (Cat. No. C30010015)</span></a>.</p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'image_id' => '203', 'type' => 'Protocol', 'url' => 'files/protocols/DNA_Shearing_in_50ul_volume_for_Pico_protocol.pdf', 'slug' => 'dna-shearing-in-50ul-volume-for-pico-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-08-03 10:54:15', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 8 => array( 'id' => '66', 'name' => 'DNA shearing for Next-Generation Sequencing with the Bioruptor® Pico (2013-2019), 0.2 ml Bioruptor® microtubes and the corresponding holder', 'description' => '<p>Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, among others the precise DNA fragmentation. The Diagenode Bioruptor® Pico ensures that the DNA is efficiently sheared to an appropriate and consistent fragment size. This first step is critical to generate high quality unbiaised libraries.</p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'image_id' => '246', 'type' => 'Protocol', 'url' => 'files/protocols/protocol-dna-shearing-tubes-02ml.pdf', 'slug' => 'protocol-dna-shearing-tubes-02ml', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-08-03 10:54:51', 'created' => '2017-06-02 11:11:06', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 9 => array( 'id' => '16', 'name' => 'DNA Shearing with Bioruptor® Pico (2013-2019) - Small Volume (10 µl) ', 'description' => '<p><span>For DNA shearing we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. </span><span>No. B01200041) and the corresponding 0.1 ml Bioruptor</span><span>® </span><span>Microtubes (Cat. No. C30010015). </span></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionI
