Diagenode

15 ml Picoruptor Tubes & sonication beads

カタログ番号
フォーマット
価格
C01020031
50 rxns
$205.00

The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.

  •  お客様の声

    Diagenode has provided a Bioruptor® Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially  for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.

    Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford
  •  プロトコル集
    Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml
    Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShea...
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  •  資料
    15 ml Bioruptor® Tubes & sonication beads DATASHEET
    The 15 ml Bioruptor® Tubes & sonication beads for chromatin shearing strongly imp...
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    Which tubes for which Bioruptor®? QUICK GUIDE
    Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 15 ml Picoruptor Tubes & sonication beads (Diagenode Cat# C01020031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Transgenic mice for in vivo epigenome editing with CRISPR-based systems
    Gemberling, M. et al.
    The discovery, characterization, and adaptation of the RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR)-Cas9 system has greatly increased the ease with which genome and epigenome editing can be performed. Fusion of chromatin-modifying domains to the nuclease-deactivated form of Cas9 (dCa...

    REPROGRAMMING CBX8-PRC1 FUNCTION WITH A POSITIVE ALLOSTERICMODULATOR
    Suh, J. L. et al.
    Canonical targeting of Polycomb Repressive Complex 1 (PRC1) to repress developmental genes is mediated by cell type-specific, paralogous chromobox (CBX) proteins (CBX2, 4, 6, 7 and 8). Based on their central role in silencing and their misregulation associated with human disease including cancer, CBX proteins are at...

    Estrogen induces dynamic ERα and RING1B recruitment to control gene and enhancer activities in luminal breast cancer
    Zhang Yusheng, Chan Ho Lam, Garcia-Martinez Liliana, Karl Daniel L., Weich Natalia, Slingerland Joyce M., Verdun Ramiro E., Morey Lluis
    RING1B, a core Polycomb repressive complex 1 subunit, is a histone H2A ubiquitin ligase essential for development. RING1B is overexpressed in patients with luminal breast cancer (BC) and recruited to actively transcribed genes and enhancers co-occupied by the estrogen receptor  (ER). Whether ER-induced transcript...

    The effect of Jun dimerization on neurite outgrowth and motif binding.
    Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP
    Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regenerati...

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