Diagenode

15 ml Picoruptor Tubes & sonication beads

カタログ番号
フォーマット
価格
C01020031
50 rxns
$185.00

The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.

  •  お客様の声

    Diagenode has provided a Bioruptor® Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially  for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.

    Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford
  •  プロトコル集
    Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml
    Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin Shearing...
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  •  実験手法
    クロマチン断片
    ChIPの成功に最も重要なステップは細胞の固定と溶解、そしてクロマチンの断片化の両方です。 研究者の中には、これらのステップの両方の重要な性質を見過すことがあります。断片化工程の不一致を解消するDiagenodeのPicoruptor®は、最先端の超音波ACT(Adaptive Cavitation Technology)を駆使し、効率的にクロマチンを断片します。ACTは、穏やかかつ非常に有効な断片力を用いて、ChIP実験に必須の高いIP効率および感度を持つ最高のクロマチン品質... Read more
  •  資料
    15 ml Bioruptor® Tubes & sonication beads DATASHEET
    The 15 ml Bioruptor® Tubes & sonication beads for chromatin shearing strongly imp...
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    Which tubes for which Bioruptor®? QUICK GUIDE
    Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 15 ml Picoruptor Tubes & sonication beads (Diagenode Cat# C01020031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The effect of Jun dimerization on neurite outgrowth and motif binding.
    Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP
    Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regenerati...

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